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Research on the Regulation of Virulence by Cell-Cell Signaling in Pathogenic Bacteria

Research Project

Project/Area Number 15390140
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionKanazawa University

Principal Investigator

SHIMIZU Tohru  Kanazawa University, Graduate School of Medical Science, Bacteriology, Professor, 医学系研究科, 教授 (80235655)

Co-Investigator(Kenkyū-buntansha) OZAKI Eijirou  Kanazawa University, Graduate School of Medical Science, Bacteriology, Assistant, 医学系研究科, 助手 (40334750)
OHTANI Kaori  Kanazawa University, Graduate School of Medical Science, Bacteriology, Assistant, 医学系研究科, 助手 (30377410)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥14,400,000 (Direct Cost: ¥14,400,000)
Fiscal Year 2004: ¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 2003: ¥7,400,000 (Direct Cost: ¥7,400,000)
KeywordsClostridium perfringens / DNA microarray / cell-cell signaling / virulence / gene regulation
Research Abstract

We investigated the amino acid sequence of the vapABC genes encoding a cell-cell signaling function of Clostridium perfringens, and tried to identify the gene directly involved in the production of signal substance. We found that the amino acid sequence in the signal peptide region of vapB was quite similar to those of the peptide pheromones of other bacteria. Although peptides of 4 to 20 amino acids were synthesized based on the sequence of the VapB signal region and the synthetic peptides were added to the cultures of C.perfringens, any significant activities were not detected, suggesting that the signaling peptide might be modified. However, since the vapABC mutant of C.peifringens lacked the ability to produce the signaling molecule, we assume that the genes are involved in the synthesis of the signal molecule and are analyzing further.
We also investigated the global regulation of all genomic genes by a cell-cell communication with other bacteria by using DNA microarrays. Escherichia coli was co-cultured with C.perfringens and the effect of the cell-cell interaction on the expression of the genes in C.perfringens was analyzed on DNA microarays. However, the experiments were turned out to fail because the background signal was too high to analyze further. Some inhibitory substances might be present in the mixed culture, which made the background high in the experiments. Now we are trying to improve the experimental condition and are going to analyze the cell-cell interactions.
We tried to investigate host factors that influence the expression of C.perfringens genes by using human and horse serum. Many genes that influenced by the addition of human serum but not by horse serum were identified by DNA microarray analysis. Among these genes, virulence genes such as sialidase were included, suggesting some factors in human serum might regulate the expression of virulence in C.perfringens.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (13 results)

All 2005 2004 Other

All Journal Article (7 results) Book (1 results) Publications (5 results)

  • [Journal Article] Identification and characterization of a cell-wall anchored DNase gene in Clostridium perfringens.2005

    • Author(s)
      K.Okumura et al.
    • Journal Title

      FEMS Microbiol.Lett. 242

      Pages: 281-285

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Identification and characterization of a cell-wall anchored DNase gene in Clostridium perfringens2005

    • Author(s)
      K.Okumura, H.I.Kawsar, T.Shimizu, T.Ohta, H.Hayashi, T.Shimizu
    • Journal Title

      FEMS Microbiol.Lett. 242

      Pages: 281-285

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Organization and transcriptional regulation of myo-Inositol operon in Clostridium perfringens2004

    • Author(s)
      H.I.Kawsar et al.
    • Journal Title

      FEMS Microbiol.Lett. 235

      Pages: 289-295

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] ウェルシュ菌のゲノム構造の解析と病原遺伝子発現調節機構の解明2004

    • Author(s)
      清水 徹
    • Journal Title

      日本細菌学会雑誌 59

      Pages: 377-385

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] ウェルシュ菌のシグナル伝達機構2004

    • Author(s)
      清水 徹, 大谷 郁, 中村信一
    • Journal Title

      臨床と微生物 31

      Pages: 229-237

    • NAID

      80016700496

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] 5.クロストリジウム2004

    • Author(s)
      清水 徹
    • Journal Title

      化学と生物 42

      Pages: 674-679

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Organization and transcriptional regulation of myo-Inositol operon in Closiridium perfringens2004

    • Author(s)
      H.I.Kawsar, K.Ohtani, K.Okurnura, H.Hayashi, T.Shimizu
    • Journal Title

      FEMS Microbiol.Lett. 235

      Pages: 289-295

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Clostridium、「ゲノミクス・プロテオミクスの新展開〜生物情報の解析と応用〜」2004

    • Author(s)
      林 英生, 清水 徹
    • Total Pages
      1158
    • Publisher
      エヌ・ティー・エス
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Publications] K.Ohtani et al.: "The VirR/VirS regulatory cascade affects transcription of plasmid-encoded putative virulence genes in Clostridium perfringens strain 13"FEMS Microbiol.Lett. 222. 137-141 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 清水 徹: "クロストリジウム属(ウェルシュ菌など)"日本臨床. 61(増刊3). 671-676 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 清水 徹: "グラム陽性菌の病原遺伝子探索:ウェルシュ菌を中心に"細胞工学. 22. 1168-1172 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 清水 徹: "ウェルシュ菌ゲノム構造の解析と病原遺伝子発現調節機構の解明"日本細菌学会誌. (印刷中).

    • Related Report
      2003 Annual Research Report
  • [Publications] 清水 徹: "ウェルシュ菌のシグナル伝達"臨床と微生物. (印刷中).

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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