| Project/Area Number |
15390147
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Hokkaido Univerisity |
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Principal Investigator |
TAKADA Kenzo Hokkaido Univ., Institute for Genetic Medicine, Prof., 遺伝子病制御研究所, 教授 (30133721)
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| Co-Investigator(Kenkyū-buntansha) |
MARUO Seiji Hokkaido Univ., Institute for Genetic Medicine, Asso.Prof., 遺伝子病制御研究所, 助教授 (70292018)
IWAKIRI Dai Hokkaido Univ., Institute for Genetic Medicine, Inst., 遺伝子病制御研究所, 助手 (10307853)
NANBO Asuka Hokkaido Univ., Institute for genetic Medicine, Inst., 遺伝子病制御研究所, 助手 (60359487)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥15,400,000 (Direct Cost: ¥15,400,000)
Fiscal Year 2004: ¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 2003: ¥9,100,000 (Direct Cost: ¥9,100,000)
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| Keywords | Epstein-Barr virus / EBER / recombinant virus / Cre / loxP system / transformation / IGF-1 / IL-10 / プロモーター / 転写活性化 / 小RNA / Bリンパ球 / 組み換えウイルス |
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| Research Abstract |
It was demonstrated that Epstein-Barr virus (EBV)-encoded small RNAs (EBERs) were nonessential for B-lymphocyte growth transformation. We revisited this issue by producing a large quantity of EBER-deleted EBV by using an Akata cell system. Although the EBER-deleted virus efficiently infected B lymphocytes, its 50% transforming dose was approximately 100-fold less than that of the EBER-positive EBV. We then engineered the genome of EBER-deleted virus and generated a recombinant virus with the EBER genes reconstituted at their native locus. The resultant EBER-reconstituted EBV exhibited restored transforming ability. In addition, lymphoblastoid cell lines established with the EBER-deleted EBV grew significantly more slowly than those established with wild-type or EBER-reconstituted EBV, and the difference between the growth rates was especially highlighted when the cells were plated at low cell densities. These results clearly demonstrate that EBERs significantly contribute to the efficient growth transformation of B lymphocytes by enhancing the growth potential of transformed lymphocytes.
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