• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

INTERACTION BETWEEN MEASLES VIRUS AND INNATE IMMUNITY

Research Project

Project/Area Number 15390151
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

YANAGI Yusuke  Kyushu University, Faculty of Medicine, Professor, 大学院・医学研究院, 教授 (40182365)

Co-Investigator(Kenkyū-buntansha) MINAGAWA Hiroko  Kyushu University, Faculty of Medicine, Associate Professor, 大学院・医学研究院, 助教授 (70209823)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥14,200,000 (Direct Cost: ¥14,200,000)
Fiscal Year 2004: ¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 2003: ¥7,500,000 (Direct Cost: ¥7,500,000)
Keywordsmeasles virus / interferon
Research Abstract

To replicate efficiently in the hosts, viruses have evolved various countermeasures to the Interferon (IFN) response. The V protein of measles virus (MV) has been shown to block IFN-alpha/beta signaling. Here, the wild-type IC-B strain of MV was shown to grow comparably in the presence and absence of IFN-alpha, whereas replication of the Edmonston tag strain recovered from cloned DNA was strongly suppressed in its presence. The V protein of the IC-B strain, but not the Edmonston tag strain, blocked IFN-alpha signaling. The V protein of the Edmonston strain from the ATCC also inhibited IFN-alpha signaling. There were three amino acid differences between the V proteins of the Edmonston ATCC and tag strains, and substitutions of both residues at positions 110 and 272 were required for the Edmonston ATCC V protein to lose IFN-antagonist activity. The P protein of the IC-B strain, which shares the N-terminal 231 as residues with the V protein, also inhibited IFN-alpha signaling. Indeed, fragments comprising only those 231 residues of the IC-B and Edmonston ATCC V proteins, but not the Edmonston tag V protein, were able to block IFN-alpha signaling. However, the N-terminal region of the Edmonston tag V protein, when attached to the C-terminal region of the Edmonston ATCC V protein, inhibited IFN-alpha signaling. Taken together, our results indicate that both the N- and C-terminal regions contribute to the IFN-antagonist activity of the MV V protein.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (19 results)

All 2004 2003 Other

All Journal Article (15 results) Publications (4 results)

  • [Journal Article] Dissection of measles virus V protein in relation to its ability to block alpha/beta interferon signal transduction2004

    • Author(s)
      Ohno, S. et al.
    • Journal Title

      Journal of General Virology 85(10)

      Pages: 2991-2999

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Genetic evidence linking SAP, the X-linked lymphoproliferative gene product, to Src-related kinase FynT in TH2 cytokine regulation2004

    • Author(s)
      Davidson, D. et al.
    • Journal Title

      Immunity 21(5)

      Pages: 707-717

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Cell tropism of wild-type measles virus is affected by amino acid substitutions in the P, V and M proteins, or by a truncation in the C protein2004

    • Author(s)
      Miyajima, N. et al.
    • Journal Title

      Journal of General Virology 85(10)

      Pages: 3001-3006

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Dissection of measles virus V protein in relation to its ability to block alpha/beta interferon signal transduction.2004

    • Author(s)
      Ohno S, Ono N, Takeda M, Takeuchi K, Yanagi Y.
    • Journal Title

      J.Gen.Virol. 85

      Pages: 2991-2999

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Genetic evidence linking SAP, the X-linked lymphoproliferative gene product, to Src-related kinase FynT in TH2 cytokine regulation.2004

    • Author(s)
      Davidson D, Shi X, Zhang S, Wang H, Nemer M, Ono N, Ohno S, Yanagi Y, Veillette A.
    • Journal Title

      Immunity 21

      Pages: 707-717

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cell tropism of wild-type measles virus is affected by amino acid substitutions in the P, V and M proteins, or by a truncation in the C protein.2004

    • Author(s)
      Miyajima N, Takeda M, Tashiro M, Hashimoto K, Yanagi Y, Nagata K, Takeuchi K.
    • Journal Title

      J.Gen.Virol. 85

      Pages: 3001-3006

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Dissection of measles virus V protein in relation to its ability to block alpha/beta interferon signal transduction2004

    • Author(s)
      Ohno, s. et al.
    • Journal Title

      Journal of General Virology 85(10)

      Pages: 2991-2999

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Cell tropism of wild-type measles virus is affected by amino acid substitutions in the P.V and M proteins, or by a truncation in the C protein2004

    • Author(s)
      Miyajima, N. et al.
    • Journal Title

      Journal of General Virology 85(10)

      Pages: 3001-3006

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Histidine at position 61 and its adjacent amino acid residues are critical for the ability of SLAM (CD150) to act as a cellular receptor for measles virus2003

    • Author(s)
      Ohno, S. et al.
    • Journal Title

      Journal of General Virology 84(9)

      Pages: 2381-2388

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Efficient isolation of wild strains of canine distemper virus in Vero cells expressing canine SLAM and their adaptability to marmoset B95a cells2003

    • Author(s)
      Seki, F. et al.
    • Journal Title

      Journal of Virology 77(18)

      Pages: 9943-9950

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Receptor use by vesicular stomatitis virus pseudotypes with glycoproteins of defective variants of measles virus isolated from brains of patients with SSPE2003

    • Author(s)
      Shingai, M. et al.
    • Journal Title

      Journal of General Virology 84(8)

      Pages: 2133-2143

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Histidine at position 61 and its adjacent amino acid residues are critical for the ability of SLAM (CD 150) to act as a cellular receptor for measles virus.2003

    • Author(s)
      Ohno S, Seki F, Ono N, Yanagi Y.
    • Journal Title

      J.Gen.Virol. 84

      Pages: 2381-2388

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Efficient isolation of wild strains of canine distemper virus in Vero cells expressing canine SLAM (CD 150) and their adaptability to marmoset B95a cells.2003

    • Author(s)
      Seki F, Ono N, Yamaguchi R, Yanagi Y.
    • Journal Title

      J.Virol. 77

      Pages: 9943-9950

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Receptor use by vesicular stomatitis virus pseudotypes with glycoproteins of defective variants of measles virus isolated from brains of patients with subacute sclerosing panencephalitis.2003

    • Author(s)
      Shingai M, Ayata M, Ishida H, Matsunaga I, Katayama Y, Seya T, Tatsuo H, Yanagi Y, Ogura H.
    • Journal Title

      J.Gen.Virol. 84

      Pages: 2133-2143

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Hisitidine at position 61 and its adjacent amino acid residues are critical for the ability of SLAM (CD150) to act as a cellular receptor for measles virus2003

    • Author(s)
      Ohno, S. et al.
    • Journal Title

      Journal of General Virology 84(9)

      Pages: 2381-2388

    • Related Report
      2004 Annual Research Report
  • [Publications] Bouche, F.B.et al.: "Neutralising immunogenicity of a polyepitope antigen expressed in a transgenic food plant : a novel antigen to protect against measles"Vaccine. 21. 2074-2081 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shingai, M.et al.: "Receptor use by vesicular stomatitis virus pseudotypes with glycoproteins of defective variants of measles virus isolated from brains of patients with SSPE"Journal of General Virology. 84. 2133-2143 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Seki, F.et al.: "Efficient isolation of wild strains of canine distemper virus in Vero cells expressing canine SLAM (CD150) and their adaptability to marmoset B95a cells"Journal of Virology. 77. 9943-9950 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ohno, S.et al.: "Histidine at position 61 and its adjacent amino acid residues are critical for the ability of SLAM (CD150) to act as a cellular receptor for measles virus"Journal of General Virology. 84. 2381-2388 (2003)

    • Related Report
      2003 Annual Research Report

URL: 

Published: 2003-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi