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Study on the practical usefulness of myoglobin from the aspects of forensic diagnosis

Research Project

Project/Area Number 15390214
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Legal medicine
Research InstitutionOkayama University

Principal Investigator

MIYAISHI Satoru  Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Associate Professor, 大学院医歯薬学総合研究科, 助教授 (90239343)

Co-Investigator(Kenkyū-buntansha) ISHIZU Hideo  Kawasaki University of Medical Welfare, Faculty of Health and Welfare, Professor, 医療福祉学部, 教授 (70033157)
YAMAMOTO Yuji  Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Lecturer, 大学院医歯薬学総合研究科, 講師 (30136379)
YOSHITOME Kei  Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Assistant, 大学院医歯薬学総合研究科, 助手 (40304307)
KITAO Takashi  Ehime Prefectural University of Health Sciences, Faculty of Health Sciences, Lecturer, 健康科学部, 講師 (60218047)
TAKATA Tomoyo  Ehime Prefectural University of Health Sciences, Faculty of Health Sciences, Lecturer, 健康科学部, 講師 (70310894)
稲垣 幸代  岡山大学, 大学院・医歯薬学総合研究科, 助手 (00325086)
Project Period (FY) 2003 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥10,700,000 (Direct Cost: ¥10,700,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2005: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2004: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2003: ¥3,100,000 (Direct Cost: ¥3,100,000)
KeywordsMyogloin / Forensic diagnosis / Postmortem interval
Research Abstract

Postmortem change of blood myoglobin concentration was experimentally investigated using rabbits. We encountered peculiar specimens two times during the term of the project, which we could not determined the assayed value. Although we could neither find the reason nor establish the concrete criterion for the estimation of time since death from the myoglobin level in postmortem blood, it was concluded that this method could be useful for the determination the time within about 12 hours postmortem. In the experiment of determining postmortem interval by the quantitative analysis of DNA degradation in myoglobin gene using mouse, it became clear that the amount of amplified products had depended on the postmortem interval, the length of the fragment amplified and the temperature in which the dead body had been left. This analysis was thought to be useful for estimating the postmortem interval of some weeks after death. Such principle like this seemed to be applicable to forensic autopsy cases. Postmortem exudation of myoglobin from the striated muscle and its diffusion were also experimentally investigated using mouse. Deletion of myoglobin was observed in the striated muscle by immunostaining as very early stage of postmortem change and this substance became visible again somewhat later. Such exudation of myoglobin could occur during the routine formalin-fixation and it was suggested that the same postmortem change could be observed about some other proteins in striated muscle. This means that the criteria of microscopic diagnosis of some causes of death using formalin-fixed myocardium/skeletal muscle must be revised.

Report

(5 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (8 results)

All 2005

All Journal Article (8 results)

  • [Journal Article] ミオグロビン遺伝子の断片化を指標とした死後経過時間推定の試み2005

    • Author(s)
      稲垣幸代, 宮石智他
    • Journal Title

      日本法医学雑誌 59巻1号

      Pages: 41-41

    • NAID

      10015685535

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Quantitative analysis of DMA degradation in myoglobin gene for estimating postmortem interval2005

    • Author(s)
      Inagaki S, Miyaishi S, et al.
    • Journal Title

      Rechtsmedizin 15巻4号

      Pages: 316-316

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] ミオグロビン遺伝子の断片化を指標とした死後経過時間推定の試み2005

    • Author(s)
      稲垣幸代, 宮石智他
    • Journal Title

      DNA多型 13巻

      Pages: 185-188

    • NAID

      10015685535

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Estimation of time since death by the analysis of DNA degradation in myoglobin gene2005

    • Author(s)
      Inagaki S, Miyaishi S, et al.
    • Journal Title

      Japanese Journal of Legal Medicine (Japanese) 59(1)

      Pages: 41-41

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Quantitative analysis of DMA degradation in myoglobin gene for estimating postmortem interval2005

    • Author(s)
      Inagaki S, Miyaishi S, et al.
    • Journal Title

      Rechtsmedizin 15(4)

      Pages: 316-316

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Estimation of time since death by the analysis of DNA degradation in myoglobin gene2005

    • Author(s)
      Inagaki S, Miyaishi S, et al.
    • Journal Title

      DNA Polymorphism (Japanese) 13

      Pages: 185-188

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Quantitative analysis of DMA degradation in myoglobin gene for estimating postmortem interval (Abstract of 6 th International Symposium)2005

    • Author(s)
      Inagaki S, Miyaishi S, et al.
    • Journal Title

      Rechtsmedizin 15巻4号

      Pages: 316-316

    • Related Report
      2005 Annual Research Report
  • [Journal Article] ミオグロビン遺伝子の断片化を指標とした死後経過時間推定の試み2005

    • Author(s)
      稲垣幸代, 宮石智他
    • Journal Title

      DNA多型 13巻(印刷中)

    • NAID

      10015685535

    • Related Report
      2004 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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