| Project/Area Number |
15390283
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Gunma University |
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Principal Investigator |
KOJIMA Itaru Gunma University, Institute for Molecular and Cellular Regulation, Professor, 生体調節研究所, 教授 (60143492)
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| Co-Investigator(Kenkyū-buntansha) |
NAGASAWA Masahiro Gunma University, Institute for Molecular and Cellular Regulation, Assistant professor, 生体調節研究所, 助手 (50343083)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 2004: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 2003: ¥10,000,000 (Direct Cost: ¥10,000,000)
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| Keywords | insulin / diabetes / regenerative medicine / stem cell / cell transplantation / cell therapy / 膵β細胞 / 再生医学 / 分化誘導因子 |
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| Research Abstract |
1)We isolated pancreatic ductal cells obtained from neonatal rats and cultured in vitro. We added two differentiation factors, activin A and betacellulin, and converted them to insulin-producing cells. Using differentiated cells, we prepared pseudoislets. The insulin content and insulin secretion were much improved after the formation of pseudoislets and, in particular, insulin secretory response to glucose was markedly improved. We then transplanted these pseudoislets into the portal vein of the streptozotocin-treated diabetic nude mice. The plasma glucose concentration was improved by transplantation of the pseudoislets.
2)For regenerative therapy, stem cells should be expanded easily. We are interested in hepatic stem-like cells, which can be expanded very easily in vitro. These cells express α-fetoprotein and CK19 and convert to both hepatocytes and duct epithelial cells. We converted these cells to cells expressing insulin and PGP9.5.
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