• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of a new treatment strategy for wound healing using recombinant type VII collagen

Research Project

Project/Area Number 15390336
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

SHIMIZU Hiroshi  Hokkaido Univ., Grad.School of Med., Prof., 大学院・医学研究科, 教授 (00146672)

Co-Investigator(Kenkyū-buntansha) SAWAMURA Daisuke  Hokkaido Univ., Grad.School of Med., Asso.Prof., 大学院・医学研究科, 助教授 (60196334)
AKIYAMA Masashi  Hokkaido University Hospital, Lec., 病院・講師 (60222551)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥14,800,000 (Direct Cost: ¥14,800,000)
Fiscal Year 2004: ¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 2003: ¥8,400,000 (Direct Cost: ¥8,400,000)
Keywordswound healing / epidermolvsis bullosa / dystrophic / severe / type VII collagen / protein therapy / gene transfer / skin ulcer
Research Abstract

Type VII collagen, a non-fibrillar collagen, is a major component of anchoring fibril structures beneath the epidermal basement membrane, and is of critical importance for integrity of the cutaneous basement membrane zone. Mutations within the type VII collagen gene are associated with the dystrophic forms of epidermolysis bullosa (DEB), a group of mechanobullous diseases characterized by cutaneous fragility and tendency to form sub-basal lamina densa blisters. The patients with DEB showed delayd wound healing. The aim of this study was to clarify therapeutic effect of recombinant type VII collagen on wound healing. First, we purified a large amount of recombinant type VII collagen. We introduced the type VII collagen gene into HaCaT cells, NIH3T3 cells and 293 cells and performed selection of stable transformants by G418.293 cells expressed type VII collagen mostly among those cell lines. We could show low mobility of keratinocytes and fibroblasts from type VII collagen-gene knockout mouse and DEB patients, but addtion of recombinant type VII collagen rescued impaired mobility. Furthermore, application of recombinant type VII collagen to skin ulcer improved wound healing and we proposed type VII collagen as an anti-ulcer drug. Thus, we think that we can achieve establishment of a new strategy for treatment of wound using recombinant type VII collagen.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (49 results)

All 2005 2004 Other

All Journal Article (29 results) Publications (20 results)

  • [Journal Article] Keratotic lesions in epidermolysis bullosa simplex with mottled pigmentation.2005

    • Author(s)
      Yasukawa K, et al.
    • Journal Title

      J Am Acad Dermatol 52

      Pages: 172-173

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Detection of 1733insC mutations inan Asian family with Birt-Hogg-Dube syndrome.2005

    • Author(s)
      Kawasaki H, et al.
    • Journal Title

      Br J Dermatol i152

      Pages: 142-145

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Oculocutaneous albinism type 4 is one of the most common types of albinism in Japan.2004

    • Author(s)
      Inagaki K, et al.
    • Journal Title

      Am J Hum Genet 74

      Pages: 466-471

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] The G2028R glycine substitution mutation in COL7A1 leads to marked inter-familiar clinical heterogeneity in dominant dystrophic epidermolysis bullosa.2004

    • Author(s)
      Nakamura H, et al.
    • Journal Title

      J Dermatol Sci 34

      Pages: 195-200

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] An elderly patient with mycosis fungoides successfully treated with chronic low-dose oral etoposide therapy.2004

    • Author(s)
      Onozuka T, et al.
    • Journal Title

      Clin Exp Dermatol 29

      Pages: 91-92

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Characterization of Kdap, a protein secreted by keratinocytes.2004

    • Author(s)
      Tsuchida S, et al.
    • Journal Title

      Invest Dermatol 122

      Pages: 1225-1234

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Ultraviolet A-induced production of matrix metalloproteinase-1 is mediated by macrophage migration inhibitory factor (MIF) in human dermalfibroblasts.2004

    • Author(s)
      Watanabe H, et al.
    • Journal Title

      J Biol Chem 279

      Pages: 1676-1683

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Characterization of Kdap, a protein secreted by keratinocytes.2004

    • Author(s)
      Tsuchida S, et al.
    • Journal Title

      J Invest Dermatol 122

      Pages: 1225-1234

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Ultraviolet A-induced production of matrix metalloproteinase-1 is mediated by macrophage migration inhibitory factor(MIF) in human dermal fibroblasts.2004

    • Author(s)
      Watanabe H, et al.
    • Journal Title

      J Biol Chem 279

      Pages: 1676-1683

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Mutations in the Plectin Gene (PLEC1) Cause a Novel Clinical Subtype, Epidermolysis Bullosa Simplex Associated with Pyloric Atresia.

    • Author(s)
      Nakamura H, et al.
    • Journal Title

      J Mol Diagn (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Novel ALDH3A2 heterozygous mutations are associated with defective lamellar granule formation in a Japanese family of Sjogren-Larsson syndrome.

    • Author(s)
      Shibaki A, et al.
    • Journal Title

      J Invest Dermatol (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] DNA-based prenatal exclusion of bullous congenital ichthyosiform erythroderma at the early stage, 10-11 weeks' of pregnancy in two consequent siblings.

    • Author(s)
      Tsuji-Abe Y, et al.
    • Journal Title

      J Am Acad Dermatol (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Direct injection of plasmid DNA into the skin induces dermatitis by activation of monocytes through toll-like receptor 9

    • Author(s)
      Sawamura D, et al.
    • Journal Title

      J.Gene Med (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Three Japanese families of ichthyosis bullosa of Siemens with keratin 2e mutations - Ichthyosis bullosa of Siemens is not anextremely rare disorder compared with bullous congenital ichthyosiform erythroderma.

    • Author(s)
      Akiyama M, et al.
    • Journal Title

      Br J Dermatol (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Beta defensin-3 engineered epidermis shows highly protective effect for bacterial infection.

    • Author(s)
      Sawamura D, et al.
    • Journal Title

      Gene Therapy (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Clinical heterogeneity of 1649delG mutation in the tail domain of keratin 5 : a Japanese family with epidermolysis bullosa simplex with mottledpigmentation.

    • Author(s)
      Horiguchi Y, et al.
    • Journal Title

      J Invest Dermato (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Mutations in ABCA12, a keratinocyte lipid transporter, in harlequin ichthyosis and functional recovery by corrective gene transfer

    • Author(s)
      Akiyama M, et al.
    • Journal Title

      J Clin Invest (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Mutations in the Plectin Gene (PLEC1) Cause a Novel Clincal Subtype, Epidermolysis Bullosa Simplex Associated with Pyloric Atresia.

    • Author(s)
      Nakamura H, et al.
    • Journal Title

      J Mol Diagn (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Novel ALDH3A2 heterozygous mutations are associated with defective lamellar granule formation in a Japanese family of Sjogren-Larsson syndrome.

    • Author(s)
      Shibaki A, et al.
    • Journal Title

      J Invest Dermatol (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] DNA-based prenatal exclusion of bullous congenital ichthyosiform erythroderma at the early stage, 10-11 weeks' of pregnancy in two consequent siblings.

    • Author(s)
      Tsuji-Abe Y, et al.
    • Journal Title

      J Am Acad Dermatol (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Direct injection of plasmid DNA into the skin induces dermatitis by activation of monocytes through toll-like receptor 9.

    • Author(s)
      Sawamura D, et al.
    • Journal Title

      J.Gene Med (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Three Japanese families of ichthyosis bullosa of Siemens with keratin 2e mutations-lchthyosis bullosa of Siemens is not an extremely rare disorder compared with bullous congenital ichthyosiform erythroderma.

    • Author(s)
      Akiyama M, et al.
    • Journal Title

      Br J Dermatol (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Beta defensin-3 engineered epidermis shows highly protective effect for bacterial infection.

    • Author(s)
      Sawamura D, et al.
    • Journal Title

      Gene Therapy (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Clinical heterogeneity of 1649delG mutation in the tail domain of keratin 5 : a Japanese family with epidermolysis bullosa simplex with mottled pigmentation.

    • Author(s)
      Horiguchi Y, et al.
    • Journal Title

      J Invest Dermatol (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Mutations in ABCA12, a keratinocyte lipid transporter, in harlequin ichthyosis and functional recovery by corrective gene transfer.

    • Author(s)
      Akiyama M, et al.
    • Journal Title

      J Clinlnvest (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Epidermolysis bullosa simplex in Oriental ; genetic studies in 19 patients.

    • Author(s)
      Yasukawa K, et al.
    • Journal Title

      Br J Dermatol. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Mutations in the Plectin Gene(PLEC1) Cause a Novel Clincal Subtype, Epidermolysis Bullosa Simplex Associated with Pyloric Atresia.

    • Author(s)
      Nakamura H, et al.
    • Journal Title

      J Mol Diagn (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Novel ALDH3A2 heterozygous mutations are associated with defective lamellar granule formation in a Japanese family of Sjogren-Larsson syndrome.

    • Author(s)
      Shibaki A, et al.
    • Journal Title

      J Invest Dermatol (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] DNA-based prenatal exclusion of bullous congenital ichthyosiform erythroderma at the early stage. 10-11 weeks' of pregnancy in two consequent siblings.

    • Author(s)
      Tsuji-Abe Y, et al.
    • Journal Title

      J Am Acad Dermatol (in press)

    • Related Report
      2004 Annual Research Report
  • [Publications] Arita K, et al.: "Squamous cell carcinoma in a patien with non-bullous congenital ichthyosiform erythroderma"Br J Dermatol. 148. 363-384 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shimizu T, et al.: "Cetirizine, an H1-receptor antagonist, suppresses the expression of macrophage migration inhibitory factor (MIF) : Its potential anti-inflammatory action"Clin Exp Allergy. 34. 103-109 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Matsuzaki Y, et al.: "Keratinocyte responsive element 3 : anaylsis of a keratinocyte-specific regulatory sequence in the 230 kDa bullous pemhigoid antigen promoter"J Invest Dermatol. 120. 308-312 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sawamura D, et al.: "Identification of COL7A1 alternative splicing inserting 9 amino acid residues into the fibronectin type III linker domain"J Invest Dermatol. 120. 942-948 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sawamura D, et al.: "Epidermolysis bullosa : directions for future research and new challenges for treatment"Arch Dermatol Res. 295 Suppl 1. S34-S42 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kawasaki H, et al.: "Squamous cell carcinoma developing in a 12-year-old boy with nonHallopeau-Siemens recessive dystrophic epidermolysis bullosa"Br J Dermatol. 148. 1047-1050 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Liu Y, et al.: "Immunofluorescence studies using skin sections of recessive dystrophic epidermolysis bullosa patients indicated that the antigen of ante-p200 pemphigoid is not a fragment of type VII collagen"J Dermatol Sci. 32. 125-129 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] McMillan JR, et al.: "Epidermal basement membrane zone components : ultrastructural distribution and molecular interactions"J Dermatol Sci. 31. 169-177 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] McMillan JR, et al.: "Ultarastructural orientation of laminin 5 in the epidermal basement membrane : an updated model for basement membrane organization"J Histochem Cytochem. 51. 1299-1306 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] McMillan JR, et al.: "Alterations in desmosome size and number coincide with the loss of keratinocyte cohesion in skin with homozygous and heterozygous defects in the desmosomal protein plakophilin 1"J Invest Dermatol. 121. 96-103 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] McMillan JR, et al.: "Immunomapping of EBA sera to multiple epitopes on collagen VII : further evidence that anchoring fibrils originate and terminate in the lamina densa"Exp Dermatol. 12. 261-267 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Siegel DH, et al.: "Loss of kindlin-1, a human homolog of the Caenorhabditis elegans actin-extracellular-matrix linker protein UNC-112, causes Kindler syndrome"Am J Hum Genet. 73. 174-187 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tomita Y, et al.: "Simultaneous occurrence of three squamaous cell carcinomas in a recessive dystrophic epidermolysis bullosa patient"Acta Derm Venereol. 83. 225-226 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tsuji Y, et al.: "Changing pattern of deiminated proteins in developing human epidermis"J Invest Dermatol. 120. 817-822 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Matsuyama N, et al.: "The dinucleotide microsatellite polymorphism of the IFNAR1 gene promoter correlates with responsiveness of hepatitis C patients to interferon"Hepatol Res. 25. 221-225 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Satoh M, et al.: "Innate apoptosis of human B lymphoblasts transformed by Epstein-Barr virus : modulation by cellular immortalization and senescence"Cell Struct Funct. 28. 61-70 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Akiyama M, et al.: "Truncation of CGI-58 Protein Causes Malformation of Lamellar Granules Resulting in Ichthyosis in Dorfman-Chanarin Syndrome"J Invest Dermatol. 121. 1029-1034 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Arita K, et al.: "Severely hyperkeratotic erythroderma associated with Hogkin's diseas-Dose high serum G-CSFcontribute to the formation of skin lesions?"J Am Acad Dermatol. 49. 772-773 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sasaki H, et al.: "A novel Sp1-family-related cis-acting element for transcription of type VII collagen gene(COL7A1)"J Dermaol Sci. 32. 239-242 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sato-Matsumura KC, et al.: "A novel insertion mutation in COL7A1 identified in Hallopeau-Siemens recessive dystrophic epidermolysis bullosa"Acta Dermato-Venereol. 83. 137-138 (2003)

    • Related Report
      2003 Annual Research Report

URL: 

Published: 2003-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi