| Project/Area Number |
15390337
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
SAWAMURA Daisuke Hokkaido Univ., Grad.School of Med., Asso.Prof., 大学院・医学研究科, 助教授 (60196334)
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| Co-Investigator(Kenkyū-buntansha) |
TABATA Yasuhiko Kyoto University, Frontier Medical Sciences, Prof., 再生医科学研究所, 教授 (50211371)
TAKAGAKI Keiichi Hirosaki Univ., School of Med., Prof., 医学部, 教授 (70163160)
SHIMIZU Hiroshi Hokkaido Univ., Grad.School of Med., Prof., 大学院・医学研究科, 教授 (00146672)
AKIYAMA Masashi Hokkaido University Hospital, Lec., 病院・講師 (60222551)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥14,700,000 (Direct Cost: ¥14,700,000)
Fiscal Year 2004: ¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 2003: ¥8,000,000 (Direct Cost: ¥8,000,000)
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| Keywords | epidermolysis bullosa / dystrophic / severe / type VII collagen / laminin 5 / protein therapy / gene transfer / dermis |
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| Research Abstract |
Epidermolysis bullosa is caused by mutations in the genes encoding structural proteins of basement membrane zone. Especially, the subtypes resulting from type VII collagen and laminin 5 genes showed severe phenotype and low quality of life. These structural proteins are thought to be produced by epidermal keratinocytes. Many dermatological investigators intend to introduce the causative genes to keratinocytes and to supply those gene products to basement membrane. The aim of this study was to establish a system in which type VII collagen and laminin 5 or their genes were continuously delivered from the dermal side to the basement membrane zone. We succeeded in purification of a large amount of recombinant type VII collagen last year. The injection of the protein induced deposition of type VII collagen in the basement membrane zone. Also injection of gene-transferred fibroblasts enabled type VII collagen to go into the basement membrane. Furthermore, implantation of these fibroblasts in scaffold increased the amount of type VII collagen in the basement membrane zone. Thus, we think that we can achieve establishment of the system in which type VII collagen and laminin 5 or their genes are continuously delivered from the dermal side to the basement membrane zone.
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