| Project/Area Number |
15390356
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Graduate School of Comprehensive Human Sciences, University of Tsukuba |
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Principal Investigator |
TAKEDA Tohoru University of Tsukuba, Graduate School of Comprehensive Human Sciences, Assistant Professor, 大学院・人間総合科学研究科, 講師 (10197311)
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| Co-Investigator(Kenkyū-buntansha) |
YASHIRO Toru University of Tsukuba, Graduate School of Comprehensive Human Sciences, Assistant Professor, 大学院・人間総合科学研究科, 講師 (20157978)
AKATSUKA Takao Yamagata University, Faculty of Engineering, Professor, 工学部, 教授 (80091875)
YUASA Tetsuya Yamagata University, Faculty of Engineering, Associate Professor, 工学部, 助教授 (30240146)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥14,800,000 (Direct Cost: ¥14,800,000)
Fiscal Year 2004: ¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 2003: ¥8,200,000 (Direct Cost: ¥8,200,000)
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| Keywords | Fluorescent X-ray / Computed Tomography / Specific element / In vivo imaging / Functional imaging / Absorption-contrast X-ray CT / Morphological imaging / Fusion imaging |
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| Research Abstract |
In recent biomedical research, the innovation of the new imaging techniques with high-contrast and high-spatial resolution is required for molecular imaging. Fluorescent x-ray technique used in the planar mode is one of the most sensitive methods for detecting trace elements of large atomic numbers in the order of picogram. However, the specimen must be cut into thin slices and be scanned with a beam perpendicular to its surface. Fluorescent x-ray CT (FXCT) method described here combines the sensitivity of fluorescence x-ray technique and the cross-sectional description of CT. Here, we describe the in-vivo FXCT imaging and related experimental results.
FXCT system consists of a silicon (111) double crystal monochromator, an x-ray slit system, a scanning table, two high purity germanium (HPGe) detectors, and pin-diode detectors. The experiment was carried out at the bending-magnet beam line of the Tristan accumulation ring in Tsukuba, Japan. Monochromatic x-ray energy was set 37keV, The incident monochromatic x-ray was collimated into a pencil beam of 1 x 0.1mm^2 for the live objects, and less than 0.5 x 0.2 mm^2 for the pathological specimens, respectively. The data acquisition time was set 5 sec for each scanning step.
I-127 labeled IMP inside the brain of a live mouse was clearly imaged by FXCT at a 1 mm spatial resolution and a 0.1 mm slice thickness. Transmission x-ray CT that demonstrate geometrical structure of skull, can be simultaneously obtained with FXCT, so the anatomical position corresponding functional changes can be understood easily. Thus, in-vivo FXCT imaging of cerebral perfusion has succeeded for the first time in the world. FXCT could reveal the distribution of 1-127 IMP within ex-vivo brain and BMIPP within myocardium of hamster. Thus, the success of FXCT imaging at in-vivo and ex-vivo, allows starting new approach to bio-imaging research.
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