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Development of Regenerative Medicine Using Reversibly Proliferating Human Bone Marrow Mesenchymal Stem Cells

Research Project

Project/Area Number 15390378
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

TANAKA Noriaki  Okayama University, Graduate School of Medicine and Dentistry, Professor, 大学院・医歯学総合研究科, 教授 (10127566)

Co-Investigator(Kenkyū-buntansha) KURABAYASHI Yuzuru  Okayama University, Advanced Science Research Center, Associate Professor, 自然生命科学研究支援センター, 助教授 (90033373)
KOBAYASHI Naoya  Okayama University, Hospital of Medicine, Assistant, 医学部・歯学部附属病院, 助手 (10325102)
MOCHITATE Katsumi  National Institute for Environmental Studies, Environmental Health Sciences Division, Head, 環境健康研究領域, 主任研究員 (20132863)
NAKAJI Shuhei  Kuraray Medical Inc., Business Development Division, Senior Staff, 新規事業開発部, 担当部長
KOHARA Michinori  The Tokyo Metropolitan Institute of Medical Science, Head, 東京都臨床医学総合研究所, 副参事研究員 (10250218)
山辻 知樹  岡山大学, 医学部・歯学部附属病院, 医員
中西 徹  岡山大学, 大学院・医歯学総合研究科, 助教授 (30243463)
児玉 亮  産業技術総合研究所, ティッシュエンジニアリングセンター, チームリーダー
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥14,700,000 (Direct Cost: ¥14,700,000)
Fiscal Year 2004: ¥7,800,000 (Direct Cost: ¥7,800,000)
Fiscal Year 2003: ¥6,900,000 (Direct Cost: ¥6,900,000)
Keywordshuman bone marrow mesenchymal stem cells / osteocyte / adipocyte / chondrocyte / lentiviral vector / mouse embryonic stem cells / 骨髄間葉系幹細胞 / HGF
Research Abstract

Researchers have paid close attention to human bone marrow mesenchymal stem cells(HBMSCs), because HBMSCs have potentials to differentiate to various types of tissue functional cells. Using a retroviral vector SSR#197, encoding LoxP-flanked human telomerase reverse transcriptase and green fluorescence protein cDNAs, we have successfully established an immortalized HMBMSC line YKNT-12. YKNT-12 cells were able to proliferate unlimitedly, on the other hand, normal HBMSCs stopped their proliferation at population doubling 30, indicating a limited life. YKNT-12 cells efficiently differentiated to osteocytes, adipocytes, and chondrocytes by adding specific growth factors in the culture medium, respectively. To differentiate YKNK-12 cells to other-typed cells, genetic manipulation is an important. Thus, we have investigated the usefulness of a lentiviral vector for such gene manipulation in mouse embryonic stem cells(ECs). After lentiviral transduction, mouse ECs still obtained multi-differentiation potentials. Therefore, a lentiviral vector will be one of the useful tools in gene manipulation in YKNK-12 cells and human ECs.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (10 results)

All 2004 2003 Other

All Journal Article (8 results) Publications (2 results)

  • [Journal Article] Lentivirus-based gene delivery in mouse embryonic stem cells.2004

    • Author(s)
      Yoshikazu Kosaka
    • Journal Title

      Artificial Organs 28(3)

      Pages: 271-277

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Partial hepatectomy and subsequent radiation facilitates engraftment of mouse embryonic stem cells in the liver.2004

    • Author(s)
      Naoya Kobayashi
    • Journal Title

      Transplantation Proceedings 36(8)

      Pages: 2352-2354

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 間葉系幹細胞による骨や脂肪に再生2004

    • Author(s)
      小林直哉
    • Journal Title

      化学工業 55(7)

      Pages: 522-526

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Regeneration of bone and fat using mesenchymal stem cells.2004

    • Author(s)
      Naoya Kobayashi
    • Journal Title

      Chemical Industry 55(7)

      Pages: 522-526

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Partial Hepatectomy and Subsequent Radiation Facilitates Engraftment of Mouse Embryonic Stem Cells in the Liver2004

    • Author(s)
      Naoya Kobayashi
    • Journal Title

      Transplantation Proceedings 36

      Pages: 2352-2354

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Lentivirus-based Gene Delivery in Mouse Embryonic Stem Cells2004

    • Author(s)
      Yoshikazu Kosaka
    • Journal Title

      Artificial Organs 28(3)

      Pages: 271-277

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Cre/loxPシステム2003

    • Author(s)
      小林直哉
    • Journal Title

      Surgery Frontier 10(3)

      Pages: 62-65

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cre/loxP system.2003

    • Author(s)
      Naoya Kobayashi
    • Journal Title

      Surgery Frontier 10(3)

      Pages: 62-65

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Yoshikazu Kosaka, *Naoya Kobayashi, *Takuya Fukazawa et al.: "Lentivirus-based Gene Delivery in Mouse Embryonic Stem Cells"Artificial Organs. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 小林直哉 他: "Cre/loxPシステム"Surgery Frontier. 10(3). 62-65 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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