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Development of a tumor cell bank for next generation immunotherapy

Research Project

Project/Area Number 15390380
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

KATANO Mitsuo  Kyushu University, Faculty of Medical Sciences, Professor, 大学院・医学研究院, 教授 (10145203)

Co-Investigator(Kenkyū-buntansha) TANAKA Masao  Kyushu University, Faculty of Medical Sciences, Professor, 大学院・医学研究院, 教授 (30163570)
SUEISHI Katsuo  Kyushu University, Faculty of Medical Sciences, Professor, 大学院・医学研究院, 教授 (70108710)
HAYASHI Jun  Kyushu University, Faculty of Medical Sciences, Professor, 大学院・医学研究院, 教授 (20150443)
NOSE Yoshiaki  Kyushu University, Faculty of Medical Sciences, Professor, 大学院・医学研究院, 教授 (20038920)
稲葉 頌一  九州大学, 大学病院, 講師 (90091305)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥14,600,000 (Direct Cost: ¥14,600,000)
Fiscal Year 2004: ¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 2003: ¥9,100,000 (Direct Cost: ¥9,100,000)
KeywordsTumor cells for treatment / Tumor cell bank / Immunotherapy / Vaccine therapy / Solid tumors / Allo-tumor cell vaccine / Exosomes / 難治性固形腫瘍
Research Abstract

Purpose of this study is to develop a highly successful procedure for establishing tumor cell lines which conform to safety criteria of blood transfusion and to build a system to use these tumor cells for treatment of other humans (a tumor cell bank for treatments).
Data obtained during this study period are summarized as follows :
1.Establishment of tumor cell lines for treatment :
1)We prepared a 96-well culture microplate in which each wells contain different concentrations of autologous sera or effusions. By using this microplate, 6 tumor cell lines were successfully established from 32 fresh carcinoma tissues.
2)We succeeded in long-term culture of tumor cells existing in tumor tissue by using micro-gravity culture system.
2.Registration of tumor cells for treatment (tumor cell bank) :
Among the 6 tumor cell lines, we got registration permission from five patients as a tumor cell bank.
Although we got registration permission from other 4 patients, we did not succeed in the establishment of a tumor cell line. These tumor tissues were frozen and stored as associate tumor cells for treatment.
3.Clinical application of tumor cell bank :
In 2 of 3 patients who are undergoing self tumor-pulsed dendritic cell-based vaccine therapy, it was judged to be able to use non-self tumor cells registered as tumor cell bank instead of self tumor cells.
In one patient who used up self tumor cells, the vaccine therapy was continued by using non-self tumor cells registered to tumor cell bank. Through a treatment period, an antitumor immune response was maintained well, and no particular side effect was recognized.
4.Publicity of tumor cell bank :
We showed clinical data of our dendritic cell-based vaccine therapy and introduced tumor cell bank in a homepage (http://www.tumor.med.kyushu-u.ac.jp/).
5. Possibility of cell-free vaccine therapy using GMP-grade exosomes secreted from tumor cells registered to tumor cell bank was indicated.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (33 results)

All 2004 2003 Other

All Journal Article (28 results) Publications (5 results)

  • [Journal Article] A novel histoculture drug response assay with a simulated microgravity culture system2004

    • Author(s)
      Nakamura K. et al.
    • Journal Title

      Preclinica 2

      Pages: 1-8

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Three-dimensional two-layer collagen matrix gel culture model for evaluating complex biological functions of monocyte-derived dendritic cells2004

    • Author(s)
      Tasaki A. et al.
    • Journal Title

      Journal of Immunological Methods 287

      Pages: 79-90

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Interleukin-1b enhances invasive ability of gastric carcinoma through nuclear factor-kB activation2004

    • Author(s)
      Yamanaka N. et al.
    • Journal Title

      Clinical Cancer Research 10

      Pages: 1853-1859

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Hedgehog signaling pathway is a new therapeutic target for patients with breast cancer2004

    • Author(s)
      Kubo M.et al.
    • Journal Title

      Cancer Research 64

      Pages: 6071-6074

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Dendritic-cell therapy after non-myeloablative stem-cell transplantation for renal-cell carcinoma2004

    • Author(s)
      Tsugami K. et al.
    • Journal Title

      Lancet Oncology 5

      Pages: 750-752

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Increased nuclear factor-kB activation in human colorectal carcinoma and its correlation with tumor progression2004

    • Author(s)
      Kojima M. et al.
    • Journal Title

      Anticancer Research 24

      Pages: 675-681

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 免疫学を基盤とした腫瘍制御法の開発2004

    • Author(s)
      片野光男
    • Journal Title

      Biotherapy 18

      Pages: 107-114

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Monocyte-derived dendritic cells that capture dead tumor cells secrete IL-12 and TNF-a through IL-12/TNF-a/NF-kB autocrine loop2004

    • Author(s)
      Onishi H. et al.
    • Journal Title

      Cancer Immunology Immunotherapy 53

      Pages: 1093-1100

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A novel histoculture drug response assay with a simulated Microgravity culture system2004

    • Author(s)
      Nakamura K
    • Journal Title

      Preclinica 2

      Pages: 1-8

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Three-dimensional two-layer collagen matrix gel culture model for Methods Evaluating complex biological functions of monocyte-derived dendritic cells2004

    • Author(s)
      Tasaki A
    • Journal Title

      Journal of Immunological 287

      Pages: 79-90

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Interleukin-1b enhances invasive ability of gastric carcinoma through nuclear factor-kB activation2004

    • Author(s)
      Yamanaka N
    • Journal Title

      Clinical Cancer Research 10

      Pages: 1853-1859

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Hedgehog signaling pathway is a new therapeutic target for breast cancer2004

    • Author(s)
      Kubo M
    • Journal Title

      Cancer Research 64

      Pages: 6071-6074

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Dendritic-cell therapy after non-myeloablative stem-cell transplantation for renal-cell carcinoma2004

    • Author(s)
      Tsugami K
    • Journal Title

      Lancet Oncology 5

      Pages: 750-752

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Increased nuclear factor-kB activation in human colorectal carcinoma and its correlation with tumor progression2004

    • Author(s)
      Kojima M
    • Journal Title

      Anticancer Research 24

      Pages: 675-681

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Monocyte-derived dendritic cells that capture dead tumor cells Secreted IL-12 and TNF-a through IL-12/TNF-a/NF-kB autocrine loop2004

    • Author(s)
      Onishi H
    • Journal Title

      Cancer Immunology Immunotherapy 53

      Pages: 1093-1100

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Development of therapeutic strategies based on immunology against tumors2004

    • Author(s)
      Katano M
    • Journal Title

      Biotherapy 18

      Pages: 107-114

    • NAID

      120002548743

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A novel histoculture drug response assay with a simulated microgravity culture system2004

    • Author(s)
      Nakamura K. et al.
    • Journal Title

      Preclinica 2・5

      Pages: 1-8

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Interleukin-1b enhances invasive ability of gastric carcinoma through nuclear Factor-kB activation2004

    • Author(s)
      Yamanaka N. et al.
    • Journal Title

      Clinical Cancer Research 10

      Pages: 1853-1859

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Dendritic-cell therapy after non-myeloablative stem-cell transplantation for Renal-cell carcinoma2004

    • Author(s)
      Tsugami K. et al.
    • Journal Title

      Lancet Oncology 5

      Pages: 750-752

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Dendritic cell-based combined immunotherapy with autologous tumor-pulsed dendritic cell vaccine and activated T cells for cancer2003

    • Author(s)
      Morisaki T. et al.
    • Journal Title

      Human Cell 16

      Pages: 175-182

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] OK-432, a streptococcal preparation, is a new maturation factor of monocyte-derived dendritic cells for clinical use2003

    • Author(s)
      Kuroki H. et al.
    • Journal Title

      Cancer Immunology Immunotherapy 52

      Pages: 561-568

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Interferon-g suppresses transforming growth factor-b-induced invasion of gastric carcinoma cells through cross-talk of Smad pathway in a three-dimensional culture model2003

    • Author(s)
      Kuga H.et al.
    • Journal Title

      Oncogene 22

      Pages: 7838-7847

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Dendritic cell-based combined immunotherapy with autologous tumor-pulsed dendritic cell vaccine and activated T cells for cancer patients2003

    • Author(s)
      Morisaki T
    • Journal Title

      Human Cell 16

      Pages: 175-182

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Interferon-g suppresses transforming growth factor-b induced Invasion of gastric carcinoma cells through cross-talk of Smad pathway In a three-dimensional culture model2003

    • Author(s)
      Kuga H
    • Journal Title

      Oncogen 22

      Pages: 7838-7847

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Exosomes secreted from monocyte-derived dendritic cells support in vitro naive T cell survival through NF-kB activation

    • Author(s)
      Matsumoto K. et al.
    • Journal Title

      Cellular Immuology (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Exosome secreted from monocyte-derived dendritic cells support in vitro naive T cell survival through NF-kB activation

    • Author(s)
      Matsumoto K
    • Journal Title

      Cellular Immunology (In press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] OK-432, a streptococcal preparation, is a new maturation factor of monocyte-derived dendritic cells for clinical use

    • Author(s)
      Kuroki H
    • Journal Title

      Cancer Immunology Immunotherapy 52

      Pages: 561-568

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Exosomes secreted from monocyte-derived dendritic cells support in vitro naive T cell survival through NF-kB activation

    • Author(s)
      Matsumoto K. et al.
    • Journal Title

      Cellular Immunology (In press)

    • Related Report
      2004 Annual Research Report
  • [Publications] Tasaki A: "Three-dimensional two-layer collagen matrix gel culture model for evaluating complex biological functions of monocyte-derived dendritic cells."Journal of Immunological Method. In press.

    • Related Report
      2003 Annual Research Report
  • [Publications] Yamanaka N: "Interleukin-1β secreted at the site of gastric carcinoma activates nuclear factor-κB resulting in expression of matrix metalloproteinase-9 and increases invasive ability."Clinical Cancer Research. In press.

    • Related Report
      2003 Annual Research Report
  • [Publications] Morisaki T: "Dendritic cell-based combined immunotherapy with autologous tumor-pulsed dendritic cell vaccine and activated T cells for cancer patients : rationale, current progress, and perspectives"Human Cell. In press.

    • Related Report
      2003 Annual Research Report
  • [Publications] Morisaki T: "Combined immunotherapy with intracavital injection of activated lymphocytes, monocyte-derived dendritic cells and low-dose OK-432 in patients with malignant effusion"Anticancer Research. 23. 4459-4465 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kubo M: "Combination of adoptive immunotherapy with Herceptin for patients with HER2-expressing breast cancer."Anticancer Research. 23. 4443-4449 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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