| Project/Area Number |
15390403
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
HIRATA Koichi Sapporo Medical University, Department of Surgical Oncology and Gastrointestinal Surgery, Professor, 医学部, 教授 (50136959)
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| Co-Investigator(Kenkyū-buntansha) |
MITAKA Toshihiro Sapporo Medical University, Cancer Research Institute, Professor, 附属がん研究所, 教授 (50231618)
KATSURAMAKI Tadashi Sapporo Medical University, Department of Surgical Oncology and Gastrointestinal Surgery, Associate Professor, 医学部, 助教授 (50253993)
MIZUGUCHI Tohru Sapporo Medical University, Department of Surgical Oncology and Gastrointestinal Surgery, Assistant Professor, 医学部, 講師 (30347174)
TSURUMA Tetsuhiro Sapporo Medical University, Department of Surgical Oncology and Gastrointestinal Surgery, Assistant Professor, 医学部, 講師 (80381268)
NOBUOKA Takayuki Sapporo Medical University, Department of Surgical Oncology and Gastrointestinal Surgery, 医学部, 助手 (70359991)
木村 康利 札幌医科大学, 医学部, 講師 (80311893)
本間 敏男 札幌医科大学, 医学部, 助手 (30315494)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥14,100,000 (Direct Cost: ¥14,100,000)
Fiscal Year 2005: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 2003: ¥5,800,000 (Direct Cost: ¥5,800,000)
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| Keywords | Cholestasis / Bile Canaliculus / Hepatic failure / Severe Infection / Small Hepatocyte / Human Hepatocyte / Hepatoid Tissue / Hepatectomy / 毛細胆管 / 非閉塞性胆汁うっ滞 / Cooplet細胞 / トランスポーター |
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| Research Abstract |
(1)It has been suggested that the mostly irregular contractions of bile canalicular net work constituted among the hepatoid structure by cultured small hepatocytes were. This movement was presented as calculated number by the specific-soft utilization of analysis for the changes of bile canalicular contraction in vitro. Moreover, some circumstances and their influences on several transporters on membrance have been studied. Interesting results have already been published during this research schedule.
(2)The completion of hepatoid tissue in vitro by cultured small hepatocyte purified from Sprague-Dawley rat (male, 6-10 week age) liver had successfully been performed. And then the distribution of transportaters localized on membrane of colonied small hepatocytes which constituted hepatoid tissue was studied by the hemi-quantitative RT-PCR and the immunofluorescence method. Preserved small hepatocytes in refregerater had been tried to culture repeatedly and the successful results showed t
… More
hat the colonied hepatocytes were slightly enlarged during 4 weeks after the start of culture and the hepatoid tissue with trabecular patterns of bile canallculus formed among cultured hepatocytes was organized. On the analysis of RT-PCR, the up-regulations of Oatp (organic anion transporting polypeptide) family, Ntcp (Na taurocholate cotransporting polypeptide), Mrp2 (multiple resistance associated protein 2) and Besp (bile salt export pump), which of all are the up-take transporters of bilirubin and bile acids or the excretion-type transporters of them, were definitely observed. Under the TNF-α addition in culture, the down-regulation or decreased of proteins of Oatp2 and CAR (constitutive androstane receptor), was induced.
(3) Human small hepatocyte was isolated from the resected liver after hepatectomy in our department. Those procedures were performed on the material without HBV and HCV infection by serum-analysis. Successful results were experienced in all cases but wide differences in results were ascertained. Further studied would be continued. Less
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