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Exhausitive search for the identification of lung cancer specific gene splicing by microarray technique

Research Project

Project/Area Number 15390407
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Thoracic surgery
Research InstitutionTohoku University

Principal Investigator

KONDO Takashi (2004-2005)  Tohoku University, Institute of Development Aging and Cancer, Professor, 加齢医学研究所, 教授 (10195901)

佐藤 雅美 (2003)  東北大学, 加齢医学研究所, 助教授 (30250830)

Co-Investigator(Kenkyū-buntansha) SAKURADA Akira  Tohoku University, Hospital, Assistant Professor, 病院・助手 (60360872)
TABATA Toshiharu  Tohoku University, Institute of Development Aging and Cancer, Assistant Professor, 加齢医学研究所, 助手 (40361191)
遠藤 千顕  東北大学, 医学部附属病院, 助手 (80333813)
近藤 丘  東北大学, 加齢医学研究所, 教授 (10195901)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥14,900,000 (Direct Cost: ¥14,900,000)
Fiscal Year 2005: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2004: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥9,700,000 (Direct Cost: ¥9,700,000)
Keywordssmall peripheral lung adenoca. / GGO / CT M / L ratio / TIMP-2 / Ki-67 / Methylation / microarray / スプライシング / 肺癌 / CT / K-ras / 細胞株 / スプライス
Research Abstract

Peripheral small lung adenocarcinomas have been detected more frequently in these days according as CT screening has become popular. The computed tomographic M/L ratio (area of the tumor in the mediastinal computed tomographic image/area of the tumor in the lung computed tomographic image) of small peripheral lung adenocarcinoma is correlated with patient prognosis. The numbers of patients with high labeling indexes of Ki-67 and high expression of vascular endothelial growth factor, CD34, matrix metalloproteinase2, and matrix metalloproteinase9 in the solid-type group (computed tomographic M/L ratio >50%) are significantly higher than those in the faint density-group (computed tomographic M/L ratio <50%). The number of patients with high levels of CD44v6 or tissue inhibitor of matrix metalloproteinase2 staining in the faint density-type group is significantly higher than that in the solid-type group. Independent variables capable of predicting computed tomographic M/L ratio included CD34, matric metalloproteinase 2, matrix metalloproteinase 9, and tissue inhibitor of matrix metalloproteinase 2.
Aberrant methylation of DNA is an important event in carcinogenesis. A microarrray analysis of DNA methylation in the promoter regions of genes using a newly developed promoter-associated methylated DNA amplification DNA chip. About 10.9% of the cancer-related genes are hypermethylated in lung cancer cell lines. Notably, HIC1, IRF7, ASC, RIPK3, RASSF1A, FABP3, PRKCDBP, and PAX3 genes are hypermethylated in most lung cancer cell lines examined. The expression profiles of these genes correlated to the methylation profiles of the genes, indicating that the microarray analysis of DNA methylation in the promoter region of the genes is convenient for epigenetic study.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (17 results)

All 2006 2005 2004 2003 Other

All Journal Article (10 results) Publications (7 results)

  • [Journal Article] Microarray analysis of promoter methylation in lung cacers2006

    • Author(s)
      Fukasawa M, et al.
    • Journal Title

      J Human Genetics 51

      Pages: 368-374

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Microarray analysis of promoter methylation in lung cancers2006

    • Author(s)
      Fukasawa M, et al.
    • Journal Title

      J Human Genetics 51

      Pages: 368-374

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Microarray analysis of promoter methylation in lung cancers2006

    • Author(s)
      Fukasawa M, et al.
    • Journal Title

      J Human Genetics (On Line)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Computed tomographic images reflect the biologic behavior of small lung adenocarcinoma2005

    • Author(s)
      Dong B, et al.
    • Journal Title

      J Thorac Cardiovasc Surg 130

      Pages: 733-739

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Computed tomographic images reflect the biologic behavior of small lung adenocarcinoma : They correlate with cell proliferation, microvascularization, cell adhesion, degradation of extracellular matrix, and K-ras mutation2005

    • Author(s)
      Dong B, et al.
    • Journal Title

      J Thorac Cardiovasc Surg 130

      Pages: 733-739

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Chromosome 12, frequently deleted in human pancreatic cancer, may encode a tumor-suppressor gene that suppresses angiogenesis2004

    • Author(s)
      Yamanaka S, et al.
    • Journal Title

      Laboratory Investigation 84

      Pages: 1339-1351

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Chromosome 12, frequently deleted in human pancreatic cancer, may encode a tumor-suppressor gene that suppresses angiogenesis2004

    • Author(s)
      Yamanaka S, et al.
    • Journal Title

      Laboratory Invest 84

      Pages: 1339-1351

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] hnRNP B1 protein may be a possible prognostic factor in squamous cell carci2004

    • Author(s)
      Wu S, Sato M, Endo C, 他
    • Journal Title

      Lung Cancer 41

      Pages: 179-186

    • Related Report
      2004 Annual Research Report
  • [Journal Article] hnRNPB1 protein may be a possible prognostic factor in squamous cell carcinoma of the lung2003

    • Author(s)
      Wu S, et al.
    • Journal Title

      Lung Cancer 41

      Pages: 179-186

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] hnRNP B1 protein may be a possible prognostic factor in squamous cell carcinoma of the lung2003

    • Author(s)
      Wu S, et al.
    • Journal Title

      Lung Cancer 41

      Pages: 179-186

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Publications] Wu S, Sato M, Endo C, Kondo T, et al.: "hnRNPB1 protein may be a possible prognostic factor in squamous cell carcinoma of the lung, Lung Cancer"Lung Cancer. 41. 179-186 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sato M, Endo C, Kondo T, et al.: "The Natural History of Radiographically Occult Bronchogenic Squamous Cell Carcinoma : A Retrospective Study of Overdiagnosis Bias"Chest. (In press).

    • Related Report
      2003 Annual Research Report
  • [Publications] Nakamura Y, Sakurada A, Sato M, Endo C, Watanabe S, Kondo T: "Directions of metachronal waves in large bronchi are different between hum an beings and quadrupeds"J O Bronchology. (In press).

    • Related Report
      2003 Annual Research Report
  • [Publications] Oyaizu T, Okada Y, Shoji W, Matumura Y, Shimada K, Sado T, Sato M, Kondo T: "Reduction of recipient macrophages by gadolinium chloride prevents development of oblierative airway disease In a rat model of heterotopic tracheal transplantaion"Transplantation. 76. 1214-1220 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takahasi H, Sagawa M, Sato M, Sakurada A, Endo C, Kondo T: "A prospective evaluation of transbronchial ultrasonography for assessment of depth of invasion in early bronchogenic squamous cell carcinoma"Lung Cancer. 42. 43-49 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kato H, Furukawa K, Sato M, Okunaka T, Kusunoki Y, Kawahara M, Matusi K, Shiraishi T, Horinouchi H: "Phase II clinical trial of photodynamic therapy using mono-L-aspartyl chlorine e6 and diode laser for early superficial squamous cell carcinoma of the lung"Lung Cancer. 42. 103-111 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 佐藤雅美他: "EBMの手法による肺癌診療ガイドライン"EBMの手法による肺癌の診療ガイドライン策定に関する研究班. 176 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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