• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of an effective neural stem cell transplantation method against ischemic brain diseases

Research Project

Project/Area Number 15390434
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionHamamatsu University School of Medicine

Principal Investigator

SATO Kohji  Hamamatsu University School of Medicine, Professor, 医学部, 教授 (80235340)

Co-Investigator(Kenkyū-buntansha) OHNO Koji  Hamamatsu University School of Medicine, Associated Professor, 医学部, 助教授 (90263277)
MIKAWA Sumiko  Hamamatsu University School of Medicine, Assistant, 医学部, 助手 (70359743)
渡部 和男  浜松医科大学, 医学部, 助手 (60107828)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥13,500,000 (Direct Cost: ¥13,500,000)
Fiscal Year 2005: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2004: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2003: ¥7,100,000 (Direct Cost: ¥7,100,000)
Keywordsneural stem cell / transplantation / bone morphogenic protein / neuron / astrocyte
Research Abstract

Neural stem cell transplantation occupies the interest of many researchers who want to cure neuronal and psychological diseases, such as ischemic brain diseases, Parkinson's diseases, and schizophrenia. However, an important issue is that most of transplanted neural stem cells differentiate into glial cells, and only little cells become neuronal cells. One of the reasons is that environmental cues determine the outcome of the differentiation. To overcome this issue, we tried to develop a new neural stem cell transplantation method using a new protein, Kir, which is an antagonist of bone morphogenetic proteins (BMPs). In vitro experiments, we have clearly shown that Kjr protein preferentially differentiates neural stem cells into neural cells. To investingate this effect in iv vivo models, we employed three different ischemic models, i.e. a gerbil 3-min ischemic model, a rat midde cerebral artery occulusion model, and a rat secondary thalami-degeneration model. We transplanted neural stem cells with Kjr protein into these model brains and evaluate the differentiation profiles of these cells using double immunostaing method. We found that Kjr-administered groups showed higher rates of differentiated neuronal cells, suggesting that Kjr is a promising tool for developing an effective neural stem cell transplatation technique.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (25 results)

All 2006 2005 2004 2003 Other

All Journal Article (21 results) Publications (4 results)

  • [Journal Article] Expression of Golf in the rat placenta : possible implication in olfactory receptor transduction.2006

    • Author(s)
      Itakura, S.
    • Journal Title

      Placenta 277

      Pages: 103-108

    • Related Report
      2005 Annual Research Report
  • [Journal Article] The differential expression patterns of messenger RNAs encoding Nogo-A and Nogo-receptor in the rat central nervous system.2005

    • Author(s)
      Hasegawa T., Ohno K., Sano M., Omura T., Kumiko Omura K., Nagano A., Sato K.
    • Journal Title

      Mol. Bran Res. 133

      Pages: 119-130

    • NAID

      120005312461

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 21. Differential expression of KCC2 accounts for the differential GABA responses between relay and intrinsic neurons in the early postnatal rat olfactory bulb.2005

    • Author(s)
      Wang C., Ohno K., Furukawa T., Ueki T., Ikeda M., Fukuda A., Kohji Sato K.
    • Journal Title

      Eur. J. Neurosci 21

      Pages: 1449-1455

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The differential expression patterns of messenger RNAs encoding Nogo-A and Nogo-receptor in the rat central nervous system.2005

    • Author(s)
      Hasegawa T., Ohno K., Sano M., Omura T., Kumiko Omura K., Nagano A., Sato K.
    • Journal Title

      Mol.Bran Res. 133

      Pages: 119-130

    • NAID

      120005312461

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Differential expression of KCC2 accounts for the differential GABA responses between relay and intrinsic neurons in the early postnatal rat olfactory bulb.2005

    • Author(s)
      Wang C., Ohno K., Furukawa T., Ueki T., Ikeda M., Fukuda A., Kohji Sato K.
    • Journal Title

      Eur.J.Neurosci 21

      Pages: 1449-1455

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Changes in expression of prosaposin in the rat facial nerve nucleus after facial nerve transection.2005

    • Author(s)
      Unuma, K.
    • Journal Title

      Neurosci.Res. 52

      Pages: 220-227

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Differential functional expression of cation-Cl- cotransporter mRNAs (KCC1, KCC2, and NKCC1) In rat trigeminal nervous system.2005

    • Author(s)
      Toyoda H.
    • Journal Title

      Mol.Bran Res. 133(1)

      Pages: 12-18

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Differential functional expression of cation-Cl- cotransporter mRNAs (KCC1, KCC2, and NKCC1) In rat trigeminal nervous system.2005

    • Author(s)
      Hasegawa T.
    • Journal Title

      Mol.Bran Res. 133(1)

      Pages: 119-130

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Essential role of endogenous tissue plasminogen activator via matrix metalloproteinase 9 induction and expression on heparin-produced cerebral hemorrhage after cerebral ischemia in mice.2004

    • Author(s)
      Zhao B.Q., Ikeda Y., Ihara H., Urano T., Fan W., Mikawa S., Suzuki Y., Kondo K., Sato K., Nagai N., Umemura K.
    • Journal Title

      Blood 103

      Pages: 2610-2616

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Brain derived neurotrophic factor rescues neuronal death induced by methamphetamine.2004

    • Author(s)
      Matsuzaki H., Namikawa K., Kiyama H., Mori N., Sato K.
    • Journal Title

      Biol. Psychi. 55

      Pages: 52-60

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Glycine cleavage system in neurogenic regions.2004

    • Author(s)
      Ichinohe A., Kure S., Mikawa S., Ueki T., Kojima K., Fujiwara K., Iinuma K., Matsubara Y., Kohji Sato K.
    • Journal Title

      Eur. J. Neurosci. 19

      Pages: 2365-2370

    • NAID

      120005312462

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Essential role of endogenous tissue plasminogen activator via matrix metalloproteinase 9 induction and expression on heparin-produced cerebral hemorrhage after cerebral ischemia in mice.2004

    • Author(s)
      Zhao B.Q., Ikeda Y, Ihara H., Urano T., Fan W., Mikawa S., Suzuki Y., Kondo K., Sato K., Nagai N., Umemura K.
    • Journal Title

      Blood 103

      Pages: 2610-2616

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Brain derived neurotrophic factor rescues neuronal death induced by methamphetamine.2004

    • Author(s)
      Matsuzaki H., Namikawa K., Kiyama H., Mori N., Sato K.
    • Journal Title

      Biol.Psychi. 55

      Pages: 52-60

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Glycine cleavage system in neurogenic regions.2004

    • Author(s)
      Ichinohe A., Kure S., Mikawa S., Ueki T., Kojima K., Fujiwara K., Iinuma K., Matsubara Y, Kohji Sato K.
    • Journal Title

      Eur.J.Neurosci. 19

      Pages: 2365-2370

    • NAID

      120005312462

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Synectin in the nervous system : Expression pattern and potential as a binding partner of neurotrophin receptors2004

    • Author(s)
      Kato H.
    • Journal Title

      FEBS lett. 572

      Pages: 123-128

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A novel model of occlusive thrombus formation in mice.2004

    • Author(s)
      Sasaki T.
    • Journal Title

      Laboratory Investigation 84(11)

      Pages: 1526-1532

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 2. A novel astrocyte-derived secretory factor, neurogenesin-1, provides neurogenic environmental cues for neural stem cells in the adult hippocampus.2003

    • Author(s)
      Ueki T., Tanaka M., Yamashita K., Mikawa S., Qiu Z.F., Maragakis N.J., Hevner R.F., Miura N., Sugimura H., Sato K.
    • Journal Title

      J. Neurosci. 23

      Pages: 11732-11740

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A novel astrocyte-derived secretory factor, neurogenesin-1, provides neurogenic environmental cues for neural stem cells in the adult hippocampus.2003

    • Author(s)
      Ueki T., Tanaka M., Yamashita K., Mikawa S., Qiu Z.F., Maragakis N.J., Hevner R.F., Miura N., Sugimura H., Sato K.
    • Journal Title

      J.Neurosci. 23

      Pages: 11732-11740

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] BMP4 expression in the adult rat brain.

    • Author(s)
      Mikawa, S.
    • Journal Title

      J.Comp.Neurol. (in press)

    • NAID

      120005312460

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Expression of Golf in the rat placenta : possible implication in olfactory receptor transduction.

    • Author(s)
      Itakura S.
    • Journal Title

      Placenta (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Differential expression of KCC2 accounts for the differential GABA responses between relay and intrinsic neurons in the early postnatal rat olfactory bulb.

    • Author(s)
      Wang C.
    • Journal Title

      Eur.J.Neurosci (in press)

    • Related Report
      2004 Annual Research Report
  • [Publications] Ichinohe A.: "Glycine cleavage system in neurogenic region"Eur J Neurosci. (in press).

    • Related Report
      2003 Annual Research Report
  • [Publications] Ueki T.: "A novel astrocyte-derived secretory factor, neurogenesin-1, provides neurogenic environmental cues for neural stem cells in the adult hippocampus."J.Neurosci. 23. 11732-11740 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Matsuzaki H: "Brain derived neurotrophic factor rescues neuronal death induced by methamphetamine"Biol.Psych.. 55. 52-60 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Zhao BQ: "Essential role of endogenous tissue plasminogen activator via matrix metalloproteinase 9 induction and expression on heparin-produced cerebral hemorrhage after cerebral ischemia in mice."Blood. 103. 2610-2616 (2004)

    • Related Report
      2003 Annual Research Report

URL: 

Published: 2003-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi