| Project/Area Number |
15390459
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Okayama University |
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Principal Investigator |
HIROHATA Satoshi Okayama University, Graduate School of Medicine and Dentistry, Assistant, 大学院・医歯学総合研究科, 助手 (90332791)
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| Co-Investigator(Kenkyū-buntansha) |
NINOMIYA Yoshifumi Okayama University, Graduate School of Medicine and Dentistry, Professor, 大学院・医歯学総合研究科, 教授 (70126241)
OOHASHI Toshitaka Okayama University, Graduate School of Medicine and Dentistry, Lecturer, 大学院・医歯学総合研究科, 講師 (50194262)
YONEZAWA Tomoko Okayama University, Graduate School of Medicine and Dentistry, Assistant, 大学院・医歯学総合研究科, 助手 (30304299)
NISHIDA Keiichiro Okayama University, Graduate School of Medicine and Dentistry, Associate Professor, 大学院・医歯学総合研究科, 助教授 (80284058)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 2004: ¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 2003: ¥10,700,000 (Direct Cost: ¥10,700,000)
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| Keywords | aggrecanase / rheumatoid arthritis / matrix metalloprotease |
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| Research Abstract |
1 Expression analysis of novel aggrecanase
RNA was extracted from IL-1 b stimulated chondrosarcoma cells and chondrocytes and the aggrecanase expressions were analyzed by quantitative RT-PCR. When compared with fibroblasts, chondrosarcoma cells and chondrocytes showed higher induction of novel aggrecanase, thus indicating this novel aggrecanase is IL-1 induced specifically in cartilage cells. When chondrosarcoma cells were stimulated with IL-1b and TNFa, novel aggrecanase induced synergistically.
2 Antibody against novel aggrecanase
We raised a polyclonal antibody against novel aggrecanase, and checked its specificity by Western blotting. A novel aggrecanase was induced by IL-1b stimulation by Western blot analysis.
3 KO mouse
We got hetero mice
4 Signal mechanism for novel aggrecanase induction
MAP kinase inhibitor(PD98059,SB203580) were added to IL- 1b stimulated chondrosarcoma cells and the signaling pathway was determined.
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