| Project/Area Number |
15390464
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
KUBO Toshikazu Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Professor, 医学研究科, 教授 (20178031)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Kenji Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Assistant, 医学研究科, 助手 (30347447)
FUJIOKA Mikihiro Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Assistant, 医学研究科, 講師 (50360029)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥14,900,000 (Direct Cost: ¥14,900,000)
Fiscal Year 2004: ¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 2003: ¥9,200,000 (Direct Cost: ¥9,200,000)
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| Keywords | chondrocyte / intermittent hydrostatic pressure / collagen / caltilage metabolism / cytokine / bone metabolism / osteoarthritis / メカニカルストレス / アポトーシス / HSP70 |
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| Research Abstract |
Chondrocytes were isolated from the articular cartilage of rabbit joints. The cells were exposed to 0.5 Hz of intermittent hydrostatic pressure(IHP) ranging from 1-5 MPa for 1 hr. After 30 minutes of pressure loading, total RNA was extracted and analyzed by competitive RT-PCR for mRNA expression relating to cartilage metabolism. The expression of proteoglycan did not changed following the exposure to 5 MPa of IHP, but the expression of collagen type II markedly increased. The expression of collagen type I and X were not detected in all cells. These results showed that the exposure to 5 MPa of IHP could inhibit terminal differentiation of chondrocytes and enhance cartilage metabolism. Five MPa of IHP had positive effect on cartilage metabolism.
Human periodontal ligament(hPDL) cells were also loaded to 0.5 Hz of IHP ranging from 1-6 MPa for 1 hr. The exposure to 6 MPa of IHP enhanced the expression of IL-6, IL-8, RANKL and OPG. IL-6 and IL-8 are inflammatory cytokines, and RANKL and OPG are cytokines relating to osteoclasts differentiation. Six MPa of hydrostatic pressure is reported as a human physiological occlusal pressure level. These results demonstrated that physiological IHP had effect on inflammatory cytokines expression and bone metabolism.
These findings provide a clue to elucidation of a part of the pathogenesis of osteoarthritis.
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