• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Elucidation of the receptor mechanisms involved in the actions of anesthetics using DNA decoy as a transcription inhibitor.

Research Project

Project/Area Number 15390474
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionOsaka University

Principal Investigator

KAMIBAYASHI Takahiko  Osaka University, Graduate School of Medicine, Assistant, 医学系研究科, 助手 (10273640)

Co-Investigator(Kenkyū-buntansha) HAYASHI Yukio  Osaka University, Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (60294063)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥14,500,000 (Direct Cost: ¥14,500,000)
Fiscal Year 2005: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 2004: ¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 2003: ¥4,600,000 (Direct Cost: ¥4,600,000)
Keywordsbrain nucleus / inhibition of transcription / analgesia / adrenergic receptor / dexmedetomidine / epinephrine-arrhythmias / I receptor / diabetes mellitus / デコイ型核酸 / siRNA
Research Abstract

1. Modification of the effects of dexmedetomidine by DNA decoy.
After confirming the anti-arrhythmic and hypnotic effects of a novel alpha2 adrenergic agonist dexmedetomidine administered into locus coeruleus of rats, we tried to elucidate the responsible subtype of alpha2 adrenergic receptor to the effects of dexmedetomidine. We administered DNA decoys, which was designed to inhibit the transcription of DNAs coding alpha2 adrenergic receptor subtypes, into locus coeruleus of rats, and then evaluated the effects of dexmedetomidine. As a result, compared with control group, each DNA decoy did not have statistically significant effects on the anti-arrhythmic and hypnotic property of dexmedetomidine. Each DNA decoy showed the tendency of reducing targeted mRNA in locus coeruleus, but the effect did not reach statistical significance. These results suggest that the direct injection of DNA decoy into locus coeruleus is not effective enough to inhibit the transcription of targeted mRNA, and s … More ome modification is needed to improve the efficacy of gene silencing.
2. Anti-arrhythmic effect of imidazoline receptor agonists
Adrenergic agonists which have imidazoline ring in their structure are known to activate I receptors. We examined anti-arrhythmic effects of various alpha2 adrenergic agonists with different affinity to I receptors, and found that I1 receptor, an I receptor subtype, plays a significant role in the anti-arrhythmic effect of alpha2 adrenergic agents. The result suggests that we should take I receptor mechanisms into consideration in order to clarify the receptor mechanisms involved in the anti-arrhythmic effect of dexmedetomidine.
3. Epinephrine-arrhythmias and diabetes mellitus
To elucidate the receptor mechanisms of the enhancement of epinephrine-induced arrhythmia by diabetes mellitus, we examined the effects of various bioactive substances on arrhythmogenic doses of epinephrine in diabetic rats. As a result, we found that ion channel mechanisms play a critical role in the enhancement of epinephrine-induced arrhythmias in diabetic animals. Less

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (3 results)

All 2005 Other

All Journal Article (3 results)

  • [Journal Article] Identification of the central imidazoline receptor subtype involved in modulation of halothane-epinephrine arrhythmias in rats.2005

    • Author(s)
      Kagawa K
    • Journal Title

      Anesth Analg 101(6)

      Pages: 1689-1694

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Diabetes mellitus reduces antiarrhythmic effect of ion channel blockers.

    • Author(s)
      Ito I
    • Journal Title

      Anesth Analg (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Diabetes mellitus reduces antiarrhythmic effect of ion channel blockers.

    • Author(s)
      Ito I
    • Journal Title

      Anesth Analg (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary

URL: 

Published: 2003-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi