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Analysis of expression pattern of the gene which is related to the radiation sensitivity in urothelial cancer and its clinical application

Research Project

Project/Area Number 15390482
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

SHINOHARA Nobuo  Hokkaido University Hospital, Lecturer, 病院, 講師 (90250422)

Co-Investigator(Kenkyū-buntansha) TADA Mitsuhiro  Hokkaido Univ., Inst.For Genetic Med., Asso.Prof., 遺伝子制御研究所, 助教授 (10241316)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥8,000,000 (Direct Cost: ¥8,000,000)
Fiscal Year 2004: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2003: ¥4,800,000 (Direct Cost: ¥4,800,000)
KeywordsBladder Cancer / Radio-therapy / Sensitivity / Transplanted tumor / p53gene / Treatment sumilation / Tumor growth delay / Resistance / マウス皮下移植モデル / 放射線感受性 / 臨床検体 / p53の変異 / 遺伝子発現 / DNAアレイ解析
Research Abstract

From the present project, the following results were obtained.
1.Initially, we evaluated radiation sensitivity in the nude mice transplanted with human bladder cancer cell lines. Utilizing this model, we set up the radiation exposure condition (single time irradiation of 5 Gy) and confirmed that tumor growth delayd reaction could be evaluated as an endpoint of radiation sensitivity.
2.The establishment of Xenogarft model : Tumor growth was observed in 18 mice (60%, 18/30). Histologically, 15 of the18 were epithelial carcinomas similar to the original tumors, resulting in a 50% (15/30) take rate. No correlation was found between the tumor take rate and the clinicopathologic features, TP53 mutational status, or Ki67 LI of the patients' tumors. Of the 15 xenografts, 11 xenografts were passed from 3 to 10 generations. TP53 mutational status remained stable during the passages, though the Ki67 LI slightly increased in the majority of xenografts. Specific growth delay after irradiation in the 4 xenografts was observed independent of the original tumor growth speed and Ki67 LI.
3.Radiological growth delay assay : To assess the usefulness of these xenografts for therapeutic simulation, we performed radiological growth-delay assay in 4 xenografts and examined the relation between the responses and the biological parameters of xenograft tumors. The radiation response considerably varied among the 4 models. Absolute growth delay ranged from 20.3+2.4 (mean+standard error) to 76.4±7.4 days. Specific growth delay (SGD) was not associated with in vivo tumor growth speed or the Ki67 Il. The smallest SGD value was observed in the No.3 model which harbored TP53 gene mutation.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (9 results)

All 2004

All Journal Article (9 results)

  • [Journal Article] Gelsolin gene silencing involving unusual hypersensitivities to dimethylsulfate and KMnO(4) in vivo footprinting on its promoter region.2004

    • Author(s)
      Haga K, et al.
    • Journal Title

      Int J Cancer. 111(6)

      Pages: 873-880

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Impact of adjuvant systemic chemotherapy on postoperative survival in patients with high-risk urothelial cancer.2004

    • Author(s)
      Suzuki S, Shinohara N, et al.
    • Journal Title

      Int J Urol. 11(7)

      Pages: 456-460

    • NAID

      50000018945

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] 進行性尿路上皮癌に対するTIN(Paclitaxel, Ifosfamide, Nedaplatin)療法2004

    • Author(s)
      鈴木 信, 篠原信雄, 他
    • Journal Title

      癌と化学療法 31(8)

      Pages: 561-565

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] 集学的治療における膀胱全摘除術と尿路変向術2004

    • Author(s)
      篠原信雄, 鈴木 信, 他
    • Journal Title

      泌尿器外科 17(2)

      Pages: 95-103

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Gene-expression profile changes correlated with tumor progression and lymph node metastasis in esophageal cancer.2004

    • Author(s)
      Tamoto E, Tada M, et al.
    • Journal Title

      Clin Cancer Res. 10(11)

      Pages: 3629-3638

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Prediction of lymph node metastasis by analysis of gene expression profiles in non-small cell lung cancer.2004

    • Author(s)
      Takada M, Tada M, et al.
    • Journal Title

      J Surg Res. 122(1)

      Pages: 61-69

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Paclitaxl, Ifomide, Nedaplatin (TIN) for patients with metastatic urothelial cancer2004

    • Author(s)
      Suzuki S, Shinohara N, et al.
    • Journal Title

      Jpn J Cancer Chemother (In Japanese) 31(4)

      Pages: 561-565

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Radical cystectomy and urinary diversion in the multidiciprinary therapy for patients with advanced bladder cancer2004

    • Author(s)
      Shinohara N, Suzuki S, et al.
    • Journal Title

      Hinyuki-Geka (In Japanese) 17(2)

      Pages: 95-103

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Gelsolin gene silencing involving unusual hypersensitivities to dimethylsulfate and KMnO(4) in vivo footprinting on its promoter region.2004

    • Author(s)
      Haga K, et al.
    • Journal Title

      Int J Cancer 111(6)

      Pages: 873-880

    • Related Report
      2004 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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