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DNA microarray analysis in transgenic mouse prostate cancer model

Research Project

Project/Area Number 15390494
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionNagasaki University

Principal Investigator

SAKAI Hideki  Nagasaki University, University Hospital of Medicine and Dentistry, Assistant Professor, 医学部・歯学部附属病院, 講師 (40235122)

Co-Investigator(Kenkyū-buntansha) IGAWA Tsukasa  Nagasaki University, Graduate School of Biomedical Sciences, Assistant Professor, 大学院・医歯薬学総合研究科, 助手 (40295069)
KANETAKE Hiroshi  Nagasaki University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (50100839)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥13,000,000 (Direct Cost: ¥13,000,000)
Fiscal Year 2004: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2003: ¥10,900,000 (Direct Cost: ¥10,900,000)
KeywordsProstate cancer / Transgenic mouse / cDNA microarray analysis / β-microseminoprotein / PSP94 / トランスジェニックマウス
Research Abstract

1.To test if the mouse β-microseminoprotein/94-amino acid prostate secretory protein (β-MSP/PSP94) gene is prostate tissue-specific, we generated two antibodies against mouse β-MSP/PSP94. Results from this study have led to the possibility of utilizing β-MSP/PSP94 as a targeting agent specifically to the prostate in a mouse experimental model.
2.To study the genetic and molecular profiles of the transgenic mouse adenocarcinoma of the prostate (TGMAP) directed by the PSP94 gene promoter/enhancer region, we performed flow cytometry and cDNA microarray analyses.
(1)Flow cytometry study of DNA ploidy and proliferation rate demonstrated that the majority of TGMAP cells were aneuploid and have a high proliferative activity (average %S+% G2M:23.0 %).
(2)TGMAP model demonstrated an unequally high abundance of neuroendocrine (NE)-related genes, and three categories of NE gene groups were further classified : NE related genes ; Ca++ metabolism, Na+,K+ channels ; cell differentiation, morphogenesis. TGMAP mice demonstrated abundant up-regulation of regulator factors involved in the signal transduction of tumor development and progression, cell membrane matrix/adhesion molecules, and tumor-associated growth factors.
3.We assessed the usefulness of three-dimensional ultrasound microimaging for measuring tumor progression in the TGMAP model. The application of three-dimensional ultrasound imaging to prostate cancer in mice showed advantages, such as high spatial resolution, and fast and uncomplicated protocols that will likely enable ultrasound to become a new microimaging modality for mouse preclinical trial studies.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (16 results)

All 2005 2004 2003 Other

All Journal Article (14 results) Publications (2 results)

  • [Journal Article] A new three-dimensional ultrasound microimaging technology for preclinical studies using a transgenic prostate cancer mouse model.2005

    • Author(s)
      Wirtzfeld LA, et al.
    • Journal Title

      Cancer Research 65・14

      Pages: 6337-6345

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A new three-dimensional ultrasound microimaging technology for preclinical studies using a transgenic prostate cancer mouse model.2005

    • Author(s)
      Wirtzfeld LA, et al.
    • Journal Title

      Cancer Research 65-14

      Pages: 6337-6345

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Knock-in of SV40 Tag oncogene in a mouse adenocarcinoma of the prostate model demonstrates advantageous features over the transgenic model2005

    • Author(s)
      Duan W, Gabril MY, Moussa M, Chan FL, Sakai H, Fong G, Xuan J
    • Journal Title

      Oncogene 24・9

      Pages: 1510-1524

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A novel knock-in prostate cancer model demonstrates biology similar to human prostate cancer and suitable for preclinical studies2005

    • Author(s)
      Gabril MY, Duan W, Wu G, Moussa M, Izawa JI, Panchal CJ, Sakai H
    • Journal Title

      Molecular Therapy 11・3

      Pages: 348-362

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Serum insulin-like growth factor binding protein-3/prostate-specific antigen ratio is a useful predictive marker in patients with advanced prostate cancer.2004

    • Author(s)
      Miyata Y, et al.
    • Journal Title

      The Prostate 54・2

      Pages: 125-132

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of insulin-like growth factor binding protein-3 before and after neoadjuvant hormonal therapy in human prostate cancer tissues : correlation with histopathologic effects and biochemical recurrence.2004

    • Author(s)
      Miyata Y, et al.
    • Journal Title

      Urology 63・6

      Pages: 1184-1190

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Coexpression of keratinocyte growth factor and its receptor in normal and prostate cancer tissues : Possible formation of autonomous andromedin loop.2004

    • Author(s)
      Aoki D, et al.
    • Journal Title

      Acta Histochemica et Cytochemica 37・6

      Pages: 379-389

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Knock-in of SV40 Tag oncogene in a mouse adenocarcinoma of the prostate model demonstrates advantageous features over the transgenic model.2004

    • Author(s)
      Duan W, et al.
    • Journal Title

      Oncogene 24・9

      Pages: 1510-1524

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Serum insulin-like growth factor binding protein-3/prostate-specific antigen ratio is a useful predictive marker in patients with advanced prostate cancer.2004

    • Author(s)
      Miyata Y, et al.
    • Journal Title

      The Prostate 54-2

      Pages: 125-132

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of insulin-like growth factor binding protein-3 before and after neoadjuvant hormonal therapy in human prostate cancer tissues : correlation with histopathologic effects and biochemical recurrence.2004

    • Author(s)
      Miyata Y, et al.
    • Journal Title

      Urology 63-6

      Pages: 1184-1190

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Coexpression of keratinocyte growth factor and its receptor in normal and prostate cancer tissues : Possible formation of autonomous andromedin loop.2004

    • Author(s)
      Aoki D, et al.
    • Journal Title

      Acta Histochemica et Cytochemica 37-6

      Pages: 379-389

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Knock-in of SV40 Tag oncogene in a mouse adenocarcinoma of the prostate model demonstrates advantageous features over the transgenic model.2004

    • Author(s)
      Duan W, et al.
    • Journal Title

      Oncogene 24-9

      Pages: 1510-1524

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Mouse PSP94 expression is prostate tissue-specific as demonstrated by a comparison of multiple antibodies against recombinant proteins.2003

    • Author(s)
      Thota A, et al.
    • Journal Title

      Journal of Cellular Biochemistry 88・5

      Pages: 999-1011

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Mouse PSP94 expression is prostate tissue-specific as demonstrated by a comparison of multiple antibodies against recombinant proteins.2003

    • Author(s)
      Thota A, et al.
    • Journal Title

      Journal of Cellular Biochemistry 88-5

      Pages: 999-1011

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Kayashima T, Yamasaki K, Yamada T, Sakai H, et al.: "The novel imprinted carboxypeptidase A4 gene (CPA4) in the 7q32 imprinting domain"Human Genetics. 112・3. 220-226 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Thota A, Sakai H(7番目), et al.: "Mouse PSP94 expression is prostate tissue-specific as demonstrated by a comparison of multiple antibodies against recombinant proteins"Journal of Cellular Biochemistry. 88・5. 999-1011 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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