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Studies on the protective mechanism of the fetal brain against hypoxic-ischemic insult during delivery

Research Project

Project/Area Number 15390507
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionYamaguchi University

Principal Investigator

NAKAMURA Shoji  Yamaguchi Univ., School of Med., Professor, 医学部, 教授 (80112051)

Co-Investigator(Kenkyū-buntansha) SAKATA Yoshiyuki  Yamaguchi Univ., School of Med., Assistant Professor, 医学部, 講師 (10034927)
FUJIOKA Takashi  Yamaguchi Univ., School of Med., Assistant Professor, 医学部, 講師 (50304473)
ISHIKAWA Akinori  Yamaguchi Univ., School of Med., Research Associate, 医学部, 助手 (40363098)
TSUCHIMOCHI Hirotsugu  Yamaguchi Univ., School of Med., Research Associate, 医学部, 助手 (60379948)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥12,700,000 (Direct Cost: ¥12,700,000)
Fiscal Year 2004: ¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 2003: ¥7,000,000 (Direct Cost: ¥7,000,000)
Keywordsasphyxia / fetal brain / umbilical cord occlusion / NO / superoxide dismutase / delivery / ischemia / oxygen / 低酸素症 / 胎児 / 脳幹 / 臍帯 / 脳血流 / 低酸素 / 活性酸素 / 酸化還元酵素 / zinc diethyldithiocarbamate / 胎児脳 / 脳性麻痺
Research Abstract

Although the fetuses experiences severe hypoxia and ischemia during delivery, the fetal brain is protected from hypoxic-ischemic insults by unknown mechanism. In a previous study, we found that at-term rat fetuses revealed a paradoxical rise in brainstem PO2 following umbilical cord occlusion, while brainstem blood flow was markedly reduced. The present experiments were designed to investigate the mechanism of this paradoxical rise in brainstem PO2 of at-term fetuses. There are two possible explanations for this phenomenon : The paradoxical rise in brainstem PO2 is due to 1) de novo generation of O2 in the brainstem or 2)O2 release from a reservoir other than blood.
To investigate possibility 1), we performed several experiments, including a) the effects of a superoxide dismutase (SOD) inhibitor on the paradoxical rise in brainstem PO2, b) Quantitative analysis of fetal brain SOD by immunoblot and c) Real-time measurement of nitrogen monoxide (NO) in the fetal brainstem following umbilical cord occlusion. In these experiments, the possibility was examined that O2 is produced from oxygen radicals which generate during hypoxic-ischemic insults. However, we did not find any evidence supporting this possibility. To investigate possibility 2), we searched chemical substances which can bind to O2. It was found that there was no such a substance in the adult rat brain, but the fetal brain remains to be investigated in future experiments. Although we also performed a morphological study on blood vessels in the fetal brain in relation to hypoxia, no significant change was found between control and hypoxia groups.
In summary, we could not reach a conclusion on the paradoxical rise in brainstem PO2 of at-term fetuses, although a variety of experiments were performed.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (18 results)

All 2004 2003 Other

All Journal Article (13 results) Publications (5 results)

  • [Journal Article] Opposite morphological responese of partially denervated cortical serotonergic and noradrenergic axons to repeated stress in adult rats.2004

    • Author(s)
      Liu Y
    • Journal Title

      Brain Research Bulltein 64

      Pages: 67-74

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Opposite morphological responses of partially denervated cortical serotonergic and noradrenergic axons to repeated stress in adult rats.2004

    • Author(s)
      Y.Liu
    • Journal Title

      Brain Research Bulltein 64

      Pages: 67-74

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Opposite morphological responses of partially denervated cortical serotonergic and noradrenergic axons to repeated stress in adult rats.2004

    • Author(s)
      Liu Y
    • Journal Title

      Brain Research Bulltein 64

      Pages: 67-74

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Convergence and interaction of hippocampal and amygdalar projections within the prefrontal cortex in the rat.2003

    • Author(s)
      Ishikawa A
    • Journal Title

      The Journal of Neuroscience 23(31)

      Pages: 9987-9995

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Interaction between serotonergic and noradrenergic axons during axonal regeneration.2003

    • Author(s)
      Liu Y
    • Journal Title

      Experimental Neurology 184

      Pages: 169-178

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Materno-fetal coordination of stress-induced Fos expression in the hypothalamic paraventricular nucleus during pregnancy.2003

    • Author(s)
      Fujioka T
    • Journal Title

      Neuroscience 118

      Pages: 409-415

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of repeated stress on regeneration of serotonergic and noradrenergic axons in the cerebral cortex of adult rats.2003

    • Author(s)
      Liu Y
    • Journal Title

      Neuroscience Letters 339(3)

      Pages: 227-230

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cortical spreading depression affects Fos expression in the hypothalamic paraventricular nucleus and the cerebral cortex of both hemispheres.2003

    • Author(s)
      Iqbal Chowdhury G M
    • Journal Title

      Neuroscience Research 45

      Pages: 149-155

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Convergence and interaction of hippocampal and amygdalar projections within the prefrontal cortex in the rat.2003

    • Author(s)
      A.Ishikawa
    • Journal Title

      The Journal of Neuroscience 23(31)

      Pages: 9987-9995

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Interaction between serotonergic and noradrenergic axons during axonal regeneration.2003

    • Author(s)
      Y.Liu
    • Journal Title

      Experimental Neurology 184

      Pages: 169-178

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Materno-fetal coordination of stress-induced Fos expression in the hypothalamic paraventricular nucleus during pregnancy.2003

    • Author(s)
      T.Fujioka
    • Journal Title

      Neuroscience 118

      Pages: 409-415

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of repeated stress on regeneration of serotonergic and noradrenergic axons in the cerebral cortex of adult rats.2003

    • Author(s)
      Y.Liu
    • Journal Title

      Neuroscience Letters 339(3)

      Pages: 227-230

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cortical spreading depression affects Fos expression in the hypothalamic paraventricular nucleus and the cerebral cortex of both hemispheres.2003

    • Author(s)
      G.M.Iqubal Chowdhury
    • Journal Title

      Neuroscience Research 45

      Pages: 149-155

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Ishikawa A: "Convergence and interaction of hippocampal and amygdalar projections within the prefrontal cortex in the rat"The Journal of Neurosience. 23(31). 9987-9995 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Liu Y: "Interaction between serotonergic and noradrenergic axons during axonal regeneration"Experimental Neurology. 184. 169-178 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Fujioka T: "Materno-fetal coordination of stress-induced Fos expression in the hypothalamic paraventricular nucleus during pregnancy"Neuroscience. 118. 409-415 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Liu Y: "Effects of repeated stress on regeneration of serotonergic and noradrenergic axons in the cerebral cortex of adult rats"Neuroscience Letters. 339(3). 227-230 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Iqbal Chowdhury G M: "Cortical spreading depression affects Fos expression in the hypothalamic paraventricular nucleus and the cerebral cortex of both hemispheres"Nouroscience Research. 45. 149-155 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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