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Elucidation for pathogenic mechanism of glaucomatous optic neuropathy and retinal ganglion cell death

Research Project

Project/Area Number 15390526
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionNiigata University

Principal Investigator

ABE Haruki  Niigata University, Graduate School of Medical and Dental Sciences, Professor, 大学院・医歯学総合研究科, 教授 (40018875)

Co-Investigator(Kenkyū-buntansha) HASEGAWA Shigeru  Niigata University, Graduate School of Medical and Dental Sciences, Associate Professor, 大学院・医歯学総合研究科, 助教授 (90228445)
FUKUCHI Takeo  Niigata University, Medical and Dental General Hospital, Assistant, 医歯学総合病院, 助手 (90240770)
SHIRAKASHI Motohiro  Niigata University, Medical and Dental General Hospital, Lecturer, 医歯学総合病院, 講師 (50242417)
UEDA Jun  Niigata University, Medical and Dental General Hospital, Resident, 医歯学総合病院, 医員
YAOEDA Kiyoshi  Niigata University, Medical and Dental General Hospital, Resident, 医歯学総合病院, 医員
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥16,700,000 (Direct Cost: ¥16,700,000)
Fiscal Year 2004: ¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 2003: ¥10,300,000 (Direct Cost: ¥10,300,000)
KeywordsGlaucoma / Ganglion Cell Death / Mitochondria / Caspase / Apoptosis / Normal-tension glaucoma / Laser Speckle Flowgraphy / Microcirculation of Optic Disc / シトクロームC / カスパーゼ3 / ATP / 軸索輸送障害 / 緑内障性視神経障害 / ラット / スキャニングレーザーポラリメータ / レーザースペックルフローグラフィー
Research Abstract

As a basic approach
We induced chronically elevated intraocular pressure in rats by injecting India ink into the anterior chamber. One week later, we performed direct laser photocoagulation (PC) of the pigmented band along the corneal limbus directly without using a gonio lens, and injected India ink again to sustain elevated IOP at above 30mmHg for 7 days. We periodically examined the apoptotic changes in the ganglion cells, as well as immunostained caspase-3 in the nerve fiber layer. Apoptotic ganglion cells began to appear on the 3rd day, and became abundant by the 5th day. In contrast, the nerve fiber layer showed a remarkable activation of caspase-3 beginning at 24 hours after IOP elevation and continued thereafter. We clarified the involvement of caspase family in the executive process of the glaucomatous ganglion cell death of the rat acute glaucoma model.
As a clinical approach
Laser speckle flowgraphy (LSFG) is a non-invasive and quantitative technique that can evaluate intraocular microcirculation. This study included 44 eyes of 44 patients with primary open-angle glaucoma (POAG) and 44 eyes of 44 patients with normal-tension glaucoma (NTG). To evaluate blood flow velocity of optic nerve head, the square blur rate (SBR) was measured by means of LSFG. The correlation between SBR and Humphrey visual field indices was evaluated with linear regression analysis. In the NTG group, SBR was positively correlated with Humphrey visual field indices (r=0.299-0.354, p=0.019-0.049). However, no significant correlation between SBR and Humphrey visual field indices was observed in the POAG group. These results suggested that the change in the circulation of the optic nerve head may be related to visual field damage in the NTG group but may be less involved in visual field damage in the POAG group.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (51 results)

All 2004 2003 Other

All Journal Article (34 results) Book (1 results) Publications (16 results)

  • [Journal Article] Localization and expression of chondromodulin-I in the rat cornea.2004

    • Author(s)
      Fukushima A
    • Journal Title

      Arch. Histol. Cytol. 66

      Pages: 445-452

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 神経線維層解析装置2004

    • Author(s)
      白柏 基宏
    • Journal Title

      あたらしい眼科 21

      Pages: 307-312

    • NAID

      10014264425

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 無縫合白内障手術後の輪状毛様体脈絡膜剥離による急性閉塞隅角緑内障の1例2004

    • Author(s)
      植木 智志
    • Journal Title

      日本眼科学会雑誌 108

      Pages: 219-225

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Statistical Evaluation of the Diagnostic accuracy of Methods Used to Determine the Progression of Visual Field Defects in Glaucoma.2004

    • Author(s)
      Haruki Abe
    • Journal Title

      Ophthalmology 111

      Pages: 2117-2125

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The Prevalence of Primary Open-Angle Glaucoma in Japanese.2004

    • Author(s)
      Haruki Abe
    • Journal Title

      Ophthalmology 111

      Pages: 1641-1648

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Variants in Optineurin Gene and Their Association with Tumor Necrosis Fautor-a Polymorphisms in Japanese Patients with Glaucoma.2004

    • Author(s)
      Haruki Abe
    • Journal Title

      Investigative Ophthalmology & Visual Science 45

      Pages: 4359-43671

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 塩酸ブナゾシン点眼の眼圧日内変動に対する効果2004

    • Author(s)
      栂野 哲哉
    • Journal Title

      あたらしい眼科 21

      Pages: 957-959

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Optic Disc Topography as Measured by Confocal Scanning LaserOphthalmoscopy and Visual Field Loss in Japanese Patients with Primary Open-Angle or Normal-Tension Glaucoma.2004

    • Author(s)
      Tomoko Nakatsue
    • Journal Title

      I Glaucoma 13

      Pages: 291-298

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 緑内障診療ガイドラインの意義2004

    • Author(s)
      阿部 春樹
    • Journal Title

      眼科 46

      Pages: 1129-1132

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Involvement of Brain-Derived Neurotrophic Factor in Early Retinal Neuropathy of Streptozotocin-Induced Diabetes in Rats.2004

    • Author(s)
      Masaaki Seki
    • Journal Title

      MIABETES 53

      Pages: 2413-2419

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Quantitative Analyses of mRNA and Protein Levels of Neurotrophin-3 in the Rat Retina During Postnatal Development and Aging.2004

    • Author(s)
      Masaaki Seki
    • Journal Title

      Jpn J Ophthalmol. 48

      Pages: 460-464

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Pressure Phosphene Tonometer ProviwTM2004

    • Author(s)
      須田生英子
    • Journal Title

      あたらしい眼科 21

      Pages: 671-674

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 原発閉塞隅角緑内障症例における生体計測2004

    • Author(s)
      澤田 英子
    • Journal Title

      あたらしい眼科 21

      Pages: 687-689

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 濾過胞再建術を行ったOverhanging Blebの3例2004

    • Author(s)
      荒木 豊
    • Journal Title

      あたらしい眼科 21

      Pages: 820-824

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 真性小眼球症に発症した原田病による続発閉塞隅角緑内障の1例2004

    • Author(s)
      中村 裕介
    • Journal Title

      あたらしい眼科 21

      Pages: 1085-1088

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 内頸動脈閉塞症に伴う血管新生緑内障の1例2004

    • Author(s)
      栂野 哲哉
    • Journal Title

      眼科紀要 55

      Pages: 889-894

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 原発開放隅角緑内障(POAG)・中期緑内障眼の治療方針と管理について2004

    • Author(s)
      福地 健郎
    • Journal Title

      Frontiers in Glaucoma 5

      Pages: 192-193

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 原発開放隅角緑内障(POAG)・後期緑内障眼の治療方針と管理について2004

    • Author(s)
      福地 健郎
    • Journal Title

      Frontiers in Glaucoma 5

      Pages: 193-195

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Localization and expression of chondromodulin-I in the rat cornea.2004

    • Author(s)
      Fukushima A
    • Journal Title

      Arch.Histol.Cytol. 66

      Pages: 445-452

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A case of acute angle-closure glaucoma secondary to annular ciliochoroidal detachment after unsutured cataract surgery2004

    • Author(s)
      Ueki S
    • Journal Title

      Nippon Ganka Gakkai Zasshi 108

      Pages: 219-225

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Statistical Evaluation of the Diagnostic accuracy of Methods Used to Determine the Progression of Visual Field Defects in Glaucoma.2004

    • Author(s)
      Abe H
    • Journal Title

      Ophthalmology 111

      Pages: 2117-2125

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The Prevalence of Primary Open-Angle Glaucoma in Japanese.2004

    • Author(s)
      Abe H
    • Journal Title

      Ophthalmology 111

      Pages: 1641-1648

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Variants in Optineurin Gene and Their Association with Tumor Necrosis Fautor-α Polymorphisms in Japanese Patients with Glaucoma.2004

    • Author(s)
      Abe H
    • Journal Title

      Investigative Ophthalmology & Visual Science 45

      Pages: 4359-4367

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effect of Bunazocin Hydrochloride Ophthalmic Solution on Circadian Rhythm of Intraocular Pressure.2004

    • Author(s)
      Togano T
    • Journal Title

      Journal of the Eye 21

      Pages: 957-959

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Optic Disc Topography as Measured by Confocal Scanning Laser Ophthalmoscopy and Visual Field Loss in Japanese Patients with Primary Open-Angle or Normal-Tension Glaucoma.2004

    • Author(s)
      Nakatsue T
    • Journal Title

      J Glaucoma 13

      Pages: 291-298

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Significance of Guidelines for Glaucoma.2004

    • Author(s)
      Abe H
    • Journal Title

      Ophthalmology 46

      Pages: 1129-1132

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Involvement of Brain-Derived Neurotrophic Factor in Early Retinal Neuropathy of Streptozotocin-Induced Diabetes in Rats.2004

    • Author(s)
      Seki M
    • Journal Title

      DIABETES 53

      Pages: 2413-2419

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Quantitative Analyses of mRNA and Protein Levels of Neurotrophin-3 in the Rat Retina During Postnatal Development and Aging.2004

    • Author(s)
      Seki M
    • Journal Title

      Jpn J Ophthalmol. 48

      Pages: 460-464

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Clinical Study of Pressure Phosphene Tonometer Proview.2004

    • Author(s)
      Suda K
    • Journal Title

      Journal of the Eye 21

      Pages: 671-674

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Biomorphometry in the Japanese Patients of Primary Angle-Closure Glaucoma.2004

    • Author(s)
      Sawada H
    • Journal Title

      Journal of the Eye 21

      Pages: 687-689

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Surgical Repair for Three Cases of Overhanging Bleb after Trabeculectomy with Mitomycin C.2004

    • Author(s)
      Araki Y
    • Journal Title

      Journal of the Eye 21

      Pages: 820-824

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Case Report of Secondary Angle-Closure Glaucoma Associated with Vogt-Koyanagi-Harada's Disease in Patient with Nanophthalmos.2004

    • Author(s)
      Nakamura Y
    • Journal Title

      Journal of the Eye 21

      Pages: 1085-1088

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A Case of Neovascular Glaucoma Due to Internal Carotid Artery Occlusion.2004

    • Author(s)
      Togano T
    • Journal Title

      Folia Ophthalmologica Japonica 55

      Pages: 889-894

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Distribution of myocilin and extracellular matrix components in the corneo-scleral meshwork of human eyes.2003

    • Author(s)
      Jun Ueda et al.
    • Journal Title

      Invest Ophthalmol Vis Sci 44(11)

      Pages: 4772-4779

    • Related Report
      2004 Annual Research Report
  • [Book] 真性小眼球症に伴う閉塞隅角緑内障の治療2004

    • Author(s)
      福地 健郎
    • Total Pages
      1
    • Publisher
      Practical Ophthalmology
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] 佐々木 亮: "放射線状角膜切開術後に発症したステロイド緑内障の1例"日本眼科学会雑誌. 107. 213-218 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 白柏 基宏: "緑内障眼における新しいGDx Accessのパラメータと視野障害との関係"あたらしい眼科. 20. 847-849 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kiyoshi Yaoeda: "Relationship between optic nerve head microcirculation and visual field loss in glaucoma"Acta Ophthalmologica Scandinavica. 81. 253-259 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 白柏 基宏: "GDx VCCによる緑内障検出力"あたらしい眼科. 20. 1019-1021 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Masaaki Seki: "BDNF is Upregulated by Postnatal Development and Visnal Experience : Quantitative and Immunohistochemical Analyses of BDNF in the Rat Retina"Investigative Ophthalmology & Visual Sciecce. 44. 3211-3218 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Keigo Sekiya: "Long-term Fundus Changes Due to Fundus Albipunctatus Associated with Mutations in the RDH5 Gene"Archives of Ophthalmology. 121. 1057-1059 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 白柏 基宏: "緑内障眼におけるGDx VCCによる網膜神経線維層厚測定"あたらしい眼科. 20. 1163-1165 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 福島 淳志: "健常眼における塩酸ブナゾシン点眼の視神経乳頭微小循環への影響"あたらしい眼科. 20. 1173-1175 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 白柏 基宏: "緑内障診療の情報交換"眼科. 45. 1137-1143 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mikio Ichibe: "Surgical Management of Retinal Detachment Associated With Myopic Macular Hole : Anatomic and Functional Status of the Macula"American Journal of Ophthalmology. 136. 277-284 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 白柏 基宏: "正常眼圧緑内障の乳頭所見"あたらしい眼科. 20. 1357-1361 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Atsushi Miki: "Decreased Activation of the Lateral Geniculate Nucleus in a Patient with Anisometropic Amblyopia Demonstrated by Functional Magnetic Resonance Imaging"Ophthalmologica. 217. 365-369 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 白柏 基宏: "スキャニングレーザーポラリメトリーによる網膜神経線維層解析"神経眼科. 20. 392-398 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 白柏 基宏: "図説 よくわかる緑内障検査法"メディカルレビュー社. 49 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 阿部 春樹: "Frontiers in Glaucoma"メディカルレビュー社. 6 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 阿部 春樹: "阿部春樹教授開講10周年記念講演会"新潟大学大学院医歯学総合研究科生体機能調節医学専攻感覚統合医学講座視覚病態学分野. 23 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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