| Project/Area Number |
15390540
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Saitama Medical School |
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Principal Investigator |
ICHIOKA Shigeru Saitama Medical School, Assistant Professor, 医学部, 助教授 (60306272)
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| Co-Investigator(Kenkyū-buntansha) |
NAKATSUKA Takashi Saitama Medical School, Professor, 医学部, 教授 (80198134)
SHIBATA Masahiro University of Tokyo, Associate Professor, 大学院・医学系研究科, 講師 (60158954)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 2004: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2003: ¥4,700,000 (Direct Cost: ¥4,700,000)
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| Keywords | Angiogenesis / Regenerative Medicine / Bone Marrow / 組織工学 / 生体制御システム / 微小循環 |
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| Research Abstract |
This study attempted to clarify the effects of therapeutic neovascularization by bone marrow cells for salvaging flaps after ischemia-reperfusion injury. Bone marrow mononuclear cell layer (endothelial progenitor cell-enriched fraction) was isolated from the mouse femur and tibia. Symmetrical double flaps were elevated in mice. Each flap topically received phosphate buffered saline (PBS) or bone marrow cells in PBS. Flaps were subjected to 6-hour ischemia and subsequent reperfusion. On the 7^<th> postoperative day, the flap survival area was measured (n=27). The mean survival area of bone marrow cells -transplanted flaps was 66.3 ± 18.0 % whereas control flaps showed a survival area of 49.7± 22.2 %. The difference was highly significant (p=0.000209). Histological examination revealed the average vascular density of bone marrow cells-transplanted flaps had significantly increased. The present study proved bone marrow cells acted with significant efficacy in promoting the survival of ischemia-reperfusion-mediated damaged tissue
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