| Project/Area Number |
15390560
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
NISHIMURA Riko Osaka University, Graduate School of Dentistry, Associate Professor, 大学院歯学研究科, 助教授 (60294112)
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| Co-Investigator(Kenkyū-buntansha) |
YONEDA Toshiyuki Osaka University, Graduate School of Dentistry, Professor, 大学院歯学研究科, 教授 (80142313)
平賀 徹 大阪大学, 大学院・歯学研究科, 講師 (70322170)
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| Project Period (FY) |
2003 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥12,800,000 (Direct Cost: ¥12,800,000)
Fiscal Year 2006: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2005: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2004: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2003: ¥3,100,000 (Direct Cost: ¥3,100,000)
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| Keywords | osteoblast / adipocyte / Osterix / Cbfa1 / Runx2 / Cbfa 1 / Cbfal / BMP-2 |
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| Research Abstract |
1.Role of Osterix in osteoblast differentiation
We found that overexpression of Osterix promoted differentiation of mesenchymal cells into osteoblasts. Microarray analysis indicated that Osterix has common and distinct target genes from Cbfa1. Therefore, our findings suggest that Osterix not only functions as a downstream of Cbfa1 but also regulates osteoblastogenesis independently of Cbfa1.
2.Cbfa1-indepenednt regulation of Osterix expression
We demonstrated that BMP2 induced Osterix expression in Cbfa1 deficient mesenchymal cells. Induction of Osterix in the cells was mediated by Smad signaling and homeobox gene.
3.Target genes of Osterix
To identify the target genes of Osterix, we performed microarray analysis and found that a transcriptional regulatory protein containing RRM motif is involved in osteoblast differentiation.
4.Role of Osterix and Cbfa1 in Ihh-mediated osteoblast differentiation
To understand the molecular basis by which Ihh stimulates osteoblast differentiation, we investigate the role of Osterix and Cbfa1 in this process. We found that Gli2, activated by Ihh, regulated expression and function of Runx2 but not those of Osterix. These results indicate that Gli2 promotes osteoblast differentiation by controlling Cbfa1.
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