Molecule gene analysis of bone absorption in bone reproduction medical care and development of bone absorption restraint medicine
| Project/Area Number |
15390614
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
SUGAHARA Toshio Okayama University, Graduate School of Medicine and Dentistry, Professor, 大学院・医歯学総合研究科, 教授 (10116048)
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| Co-Investigator(Kenkyū-buntansha) |
MISHIMA Katuaki Okayama university, Hospitals, Assistant Professor, 医学部・歯学部附属病院, 講師 (60304317)
MIZUKAWA Nobuyoshi Okayama university, Hospitals, Assistant Professor, 医学部・歯学部附属病院, 講師 (00263608)
UENO Takaaki Okayama University, Graduate School of Medicine and Dentistry, Assistant, 大学院・医歯学総合研究科, 助手 (60252996)
FUKUNAGA Jyoji Okayama University, Graduate School of Medicine and Dentistry, Assistant, 大学院・医歯学総合研究科, 助手 (10284069)
IKEGAMI Syuji Meiji Dairies Corporation, 機能評価研究部, 課長
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥14,900,000 (Direct Cost: ¥14,900,000)
Fiscal Year 2004: ¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 2003: ¥9,100,000 (Direct Cost: ¥9,100,000)
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| Keywords | osteoprosis / osteoclast / Immunosuppresant agents / 免疫柳制剤 / T細胞 / RANKL / OPG / リアルタイムPCR |
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| Research Abstract |
Intramuscular injection (1 mg/kg) did immunity restraint agent FK506 to a nude mouse and a mouse of a wild type that a T cell suffered a loss day after day on 28th and made an osteoporosis model mouse to search a cell pro-immunity and relevance with the onset of osteoporosis by immunity restraint agent. As a result, as for the bone absorption of a group of experiments by FK506, decrease of trabecula bone was recognized by a wild type more underneath than enchondral ossification, and, as for positive osteoclast, numbers increased in enchondral ossification for TRAP dyeing more clearly than a group of control again. However, as for the bone absorption in the lower part, as for the osteoporosis by immunity restraint agent, what an immunity system cell such as a T cell was related to was suggested without most than enchondral ossification with a nude mouse by there not being control and a change.
I discovered that osteoporosis recovered when we administered Propion acid bacteria fermentatio
… More
n product (PC) that was a starter of Swiss cheese to this osteoporosis model. We examined a bone absorption restraint effect from this fermentation product this time. I was able to prevent the bone absorption when I administered Propion acid bacteria fermentation product (PC) to an osteoporosis mouse by FK506.
In late years a lot of existing DHNA has a naphthoquinone frame the same as vitamins K2 attracting attention in a quantity of bone salt thing having quantity of bone improvement action within a PC.
I paid attention to this point, and we accepted bone absorption restraint same as a PC for an osteoporosis model when we administered DHNA alone. As for DHNA, the bone absorption restraint effect of a PC was able to identify a thing by DHNA from what was included in a PC abundantly.
I compared osteoclast with control when I added the B cell which was a T cell and the B220 positive that were four twist CD positive in marrow in osteoclast of a mouse namely in vitro of bone bud cell-like strike Rome cell and marrow cell with magnetic beads and increased significantly. Less
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Report
(3 results)
Research Products
(24 results)
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[Journal Article] Expression of Osteoclast Differentiation Factor (RANKL) and Osteoclastogenesis Inhibitory Factor (OPG) in Rat Osteoporosis Induced by Immunosuppressant FK506.2004
Author(s)
Jyoji Fukunaga, Yuichirou Yamaai, Eiki Yamachika, Yuzou Ishiwari, Hidetugu Tsujigiwa, Koichi Sawaki, Y.J.Lee, Takaaki Ueno, Shiho Kirino, Nobuyoshi Mizukawa, Shin Takagi, Noriyuki Nagai, Toshio Sugahara
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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