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Regenerative Medicine of the Jawbone Using Mesechymal Stem Cells and /or Bioactive Factors

Research Project

Project/Area Number 15390624
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionKanagawa Dental College

Principal Investigator

KINOSHITA Yukihiko  Kanagawa Dental College, Frontier Oral Science, Professor, 高次口腔科学研究所, 教授 (70084770)

Co-Investigator(Kenkyū-buntansha) TABATA Yasuhiko  Kyoto University, Frontier Medical Science, Professor, 再生医科学研究所, 教授 (50211371)
IKADA Yoshito  Nara Medical University, School of Medicine, Professor, 医学部教授, 教授 (00025909)
KAWASE Toshio  Kanagawa Dental College, School of Dentistry, Professor, 歯学部, 教授 (30084784)
OZONO Satoru  Kanagawa Dental College, Frontier Oral Science, Associate Professor, 高次口腔科学研究所, 助教授 (40084785)
TODOKI Kazuo  Kanagawa Dental College, School of Dentistry, Lecturer, 歯学部, 講師 (90139577)
Project Period (FY) 2003 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥14,000,000 (Direct Cost: ¥14,000,000)
Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2005: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2004: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2003: ¥7,700,000 (Direct Cost: ¥7,700,000)
KeywordsPoly(LA-CL) / statin / 17-estradiol / bFGF / PRP / PGA / regenerative medicine / jawbone / 骨髄間葉系幹細胞 / 顎骨再生 / 17-cstradiol / PTH / scaffold / β-estradiol / hPTH / 徐放 / 骨髄間葉系細胞 / statin / DDS / GBR / PLLA
Research Abstract

1. The effects of combining a porous poly(L-lactide-co-e-caprolactone)/β-TCP membrane with a bFGF incorporating gelatin sponge on bone regeneration in mandibular ridges of beagles was evaluated. The results suggested that this technique will be applicable for alveolar ridge reconstruction.
2. Compactin and Pitavastatin increased activity of alkaline phosphatase of MC3T3-E1 cells (osteoblast-like cells) and enhanced these calcification. Therefore, Pitavastatin evoked apotosis of Raw cells ( macrophage-like cells) and inhibited differentiation to osteoclasts.
Combination of bFGF and 17β-estradiol to the osteogenic medium promoted differentiation of MC3T3-E1 cells without decrease of cell proliferation.
3. β-estoradiol incorporated PLGA and hPTH incorporated PLGA showed tendency to increase alveolar ridge post extraction. BMP-2 incorporated gelatin containing β-TCP showed remarkable bone regeneration in defects of the calvaria and the mandibular ramus.
4.Combination of PRP or bFGF incorporated gelatin with particulate cancellous bone and marrow showed tendency to promote bone regeneration in the mandibular marginal resection of beagles. However, there is no significant difference between those and control. An additional experiment is needed.
5.Complex of rat bone marrow stromal cells and hydroxyapatite coated PGA/collagen sponge showed more bone formation in the subcutaneous implants than control.
6. bFGF incorporated gelatin was applied to 11 patients with periodontal disease or atrophy of the alveolar ridge for dental implant. The results showed no harmful events and good outcome.

Report

(5 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (57 results)

All 2007 2006 2005 2004 2003 Other

All Journal Article (46 results) Book (6 results) Publications (5 results)

  • [Journal Article] Age-dependent changes in the racemization ratio of aspartic acid in human alveolar bone.2007

    • Author(s)
      Ohtani S
    • Journal Title

      Archives of Oral Biology 52・1

      Pages: 4-4

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Age-dependent changes in the racemization ratio of aspartic acid in human alveolar bone2007

    • Author(s)
      Ohtani S
    • Journal Title

      Archives of Oral Biology 4

      Pages: 52-1

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Medical Do. Co2007

    • Author(s)
      Kinoshita Y
    • Journal Title

      New development of drag delivery system and the clinical appliance ed.Tabata Y

      Pages: 3-3

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Nihonigakukan2007

    • Author(s)
      Kinoshita Y
    • Journal Title

      Tissue Engineering 2006, ed, Tabata Y, Okano M

      Pages: 7-7

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Age-dependent changes in the racemization ratio of aspartic acid in human alveolar bone.2007

    • Author(s)
      Ohtani S
    • Journal Title

      Archives of Oral Biology 52

      Pages: 233-236

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Cationized gelatin delivery of a plasmid DNA expressing small interference RNA for VEGF inhibits murine squamous cell carcinoma2006

    • Author(s)
      Matsumoto G.
    • Journal Title

      Cancer Science 97・4

      Pages: 9-9

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Regenerative treatment for the Jawbone2006

    • Author(s)
      Kinoshita Y
    • Journal Title

      BKDC 34・1

      Pages: 3-3

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Tissue Engineering を応用した顎骨の再構築2006

    • Author(s)
      木下靭彦 276-279, 2006
    • Journal Title

      頭頸部癌 32・3

      Pages: 4-4

    • NAID

      10018302502

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Cationized gelatin delivery of a plasmid DNA expressing small interference RNA for VEGF inhibits murine squamous cell carcinoma2006

    • Author(s)
      Matsumoto G.
    • Journal Title

      Cancer Science 9

      Pages: 97-4

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Regenerative treatment for the Jawbone2006

    • Author(s)
      Kinoshita Y
    • Journal Title

      BKDC 3

      Pages: 34-1

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Reconstruction of the mandible based on tissue engineering2006

    • Author(s)
      Kinoshita Y
    • Journal Title

      Japanese Journal of Head and Neck Cancer 5

      Pages: 32-3

    • NAID

      10018302502

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Medical Review. Co2006

    • Author(s)
      Kinoshita Y
    • Journal Title

      Growth Factor & Regenerative Medicine, ed. Matumoto K, Tabata Y

      Pages: 6-6

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Medical DO, Co.(in print)2006

    • Author(s)
      Kinoshita Y
    • Journal Title

      Advance of cell-based regenerative medicine-Jawbone

      Pages: 7-7

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Cationized gelatin delivery of a plasmid DNA expressing small interference RNA for VEGF inhibits murine squamous cell carcinoma2006

    • Author(s)
      Matsumoto, G.
    • Journal Title

      Cancer Science 97.4

      Pages: 313-321

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Regenerative treatment for the Jawbone.2006

    • Author(s)
      Kinoshita Y.
    • Journal Title

      Bulletin KDC 34・1

      Pages: 89-91

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Tissue Engineeringを応用した顎骨の再構築2006

    • Author(s)
      木下靭彦
    • Journal Title

      頭頸部癌 32・3

      Pages: 276-279

    • NAID

      10018302502

    • Related Report
      2006 Annual Research Report
  • [Journal Article] ビバスタチンはカスパーゼ非依存性アポトーシスによりRAW細胞から破骨細胞への分化を抑制する2006

    • Author(s)
      竹山禎章
    • Journal Title

      J Oral Biosci 48・suppl

      Pages: 158-158

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Scaffolds for jawbone tissue engineering2005

    • Author(s)
      Kinoshita Y
    • Journal Title

      BKDC 33・1

      Pages: 6-6

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Bone regeneration,Effect of basic fibroblast growth factor/esterogen combinations on bone formation in rat bone marrow stromal cells.2005

    • Author(s)
      Ozono S
    • Journal Title

      BKDC 33・1

      Pages: 6-6

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Proliferation and differentiation of rat bone marrow stromal cells on poly(glycolic acid)-collagen sponge.2005

    • Author(s)
      Fujita M
    • Journal Title

      Tissue Engineering. 11・9/10

      Pages: 10-10

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Tissue Engineering を応用した下顎骨の再生医療2005

    • Author(s)
      木下靭彦 569-575, 2005.
    • Journal Title

      補綴誌 49・4

      Pages: 7-7

    • NAID

      10029761673

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 下顎骨の再生医療2005

    • Author(s)
      木下 靭彦
    • Journal Title

      頭頸部癌 31.4

      Pages: 6-6

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Scaffolds for jawbone tissue engineering2005

    • Author(s)
      Kinoshita Y
    • Journal Title

      BKDC 6

      Pages: 33-1

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Bone regeneration, Effect of basic fibroblast growth factor/esterogen combinations on bone formation in rat bone marrow stromal cells.2005

    • Author(s)
      Ozono S
    • Journal Title

      BKDC 6

      Pages: 33-1

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Proliferation and differentiation of rat bone marrow stromal cells on poly(glycolic acid)-collagen sponge2005

    • Author(s)
      Fujita M
    • Journal Title

      Tissue Engineering. 10

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Tissue engineering for jawbone2005

    • Author(s)
      Kinoshita, Y
    • Journal Title

      JJpn Prosthodont Soc

      Pages: 494-494

    • NAID

      10029761673

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Regenerative medicine of bone : Mandibular techniques2005

    • Author(s)
      Kinoshita Y
    • Journal Title

      Japanese Journal of Head and Neck Cancer 6

      Pages: 31-4

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Proliferation and differentiation of rat bone marrow stromal cells on poly(glycolic acid)-collagen sponge2005

    • Author(s)
      Motoki Fujita
    • Journal Title

      Tissue Engineering 11.9-10

      Pages: 1346-1355

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Scaffolds for Jawbone Tissue Engineering2005

    • Author(s)
      Yukihiko Kinoshita
    • Journal Title

      BKDC 33.1

      Pages: 15-20

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Tissue Engineeringを応用した下顎骨の再生医療2005

    • Author(s)
      木下靭彦
    • Journal Title

      補綴誌 49.4

      Pages: 569-575

    • NAID

      10029761673

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 下顎骨の再生医療2005

    • Author(s)
      木下靭彦
    • Journal Title

      頭頸部癌 31

      Pages: 396-401

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Reconstruction of the mandible using poly(L-lactide) mesh and autogenic particulate cancellous bone and application of dental implants2005

    • Author(s)
      Yukihiko Kinoshita
    • Journal Title

      Int.J.Oral Maxillofac.Surg 34・suppl.

      Pages: 141-142

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Scaffold for jawbone tissue engineering2005

    • Author(s)
      Yukihiko Kinoshita
    • Journal Title

      Bulletin of Kanagawa Dental College 33・1

      Pages: 15-20

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Proliferation and Differentiation of Rat Bone Marrow Stromal Cells on PGA/Collagen Sponge2005

    • Author(s)
      Motoki Fujita
    • Journal Title

      Tissue Engineering 11・9(未定)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 特集・再生医工学と高分子-顎骨の再生2004

    • Author(s)
      木下靭彦
    • Journal Title

      高分子 53・3

      Pages: 1-1

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Regenerative medicine for jawbone2004

    • Author(s)
      Kinoshita Y
    • Journal Title

      JMAJ 47・6

      Pages: 4-4

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 臨床家の望むバイオマテリアル、歯科分野-口腔外科-2004

    • Author(s)
      木下 靭彦
    • Journal Title

      バイオマテリアル 22・3

      Pages: 5-5

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Regenerative medicine for jawbone2004

    • Author(s)
      Kinoshita Y
    • Journal Title

      JMAJ 4

      Pages: 47-6

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Polymer for tissue engineering-Tissue engineering of jawbone2004

    • Author(s)
      Kinoshita Y
    • Journal Title

      Kobunshi, High polymers, Japan 53-3

      Pages: 1-1

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Desirable biomaterials for dentistry oral Surgery2004

    • Author(s)
      Kinoshita Y
    • Journal Title

      Journal of Japanese Society for Biomaterials 22.3

      Pages: 5-5

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Regenerative medicine for jawbone2004

    • Author(s)
      Yukihiko Kinoshita
    • Journal Title

      JMAJ 47巻

      Pages: 294-297

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 臨床家の望むバイオマテリアル歯科分野-口腔外科-2004

    • Author(s)
      木下 靭彦
    • Journal Title

      バイオマテリアル 22・3

      Pages: 179-183

    • Related Report
      2004 Annual Research Report
  • [Journal Article] ラット骨髄培養細胞へのエストロゲン添加効果2004

    • Author(s)
      小園 知
    • Journal Title

      JAOB 46・5

      Pages: 462-462

    • NAID

      110003163388

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Proliferation and differentiation of rat bone marrow stromal cells on hydroxyapatite coating PGA/Collagen sponge

    • Author(s)
      Kamiya S
    • Journal Title

      Tissue Engineering (発表予定)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Alveolar bone regeneration using absorbable poly(L-lactide-co-e-caprolactone)/B-TCP membrane and bFGF-Gelatin sponge.AUTHORS, TITLE OF ARTICLE

    • Author(s)
      Kinoshita Y
    • Journal Title

      Int J Oral Maxillofac Surg (in submission)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Proliferation and differentiation of rat bone marrow stromal cells on hydroxyapatite coating PGA/Collagen sponge,

    • Author(s)
      Kamiya S
    • Journal Title

      Tissue Engineering (in submission)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 進みつづける細胞移植治療の実際-再生医療の実現に向けた科学・技術と周辺要素の理解2007

    • Author(s)
      木下靭彦 共著
    • Publisher
      メディカル ドウ(印刷中)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 細胞増殖因子と再生医療(松本邦夫、田畑泰彦編)2006

    • Author(s)
      木下靭彦 共著
    • Total Pages
      6
    • Publisher
      メディカルレビュー社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] ティッシュエンジニアリング2006(田畑泰彦、岡野光夫編)2006

    • Author(s)
      木下靭彦 共著
    • Total Pages
      7
    • Publisher
      日本医学館
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 細胞増殖因子と再生医療(松本邦夫, 田畑泰彦編)2006

    • Author(s)
      木下靭彦
    • Publisher
      メディカルレビュー社
    • Related Report
      2006 Annual Research Report
  • [Book] ティッシュエンジニアリング2006(田畑泰彦, 岡野光夫編)2006

    • Author(s)
      木下靭彦
    • Publisher
      日本医学館
    • Related Report
      2006 Annual Research Report
  • [Book] ドラッグデリバリーシステムDDS技術の新たな展開とその活用法(田畑泰彦編)2003

    • Author(s)
      木下靭彦 共著
    • Total Pages
      6
    • Publisher
      メディカル ドウ
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Publications] 木下靭彦: "顎骨の再生医療-生体吸収性ポリ-L-乳酸メッシュと新鮮自家骨髄海綿骨細片を用いた下顎骨再生-"日本歯科医師会雑誌. 22. 107-110 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kinoshita Y: "Reconstruction of jawbones using poly(L-lactic acid) mesh and transplantation of particulate cancellouse bone and marrow : Long-term observation of 40 cases"Int.J.Oral.Maxillofac.Surg.. 3・Suppl1. 117 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 小園 知: "ラット骨髄培養細胞の骨形成能におけるエストロゲンの添加効果について"歯科基礎歯科医学会雑誌. 45・5. 300 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hiraoka Y: "Fabrication and biocompatibility of collagen sponge reinforced with poly(glycolic acid) fiber."Tissue Engineering. 9・6. 1101-1112 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 木下靭彦: "ドラッグデリバリーシステムDDS技術の新たな展開とその活用法"メディカル ドウ. 6 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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