| Project/Area Number |
15390642
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Periodontal dentistry
|
|---|
| Research Institution | Tokyo Medical and Dental University |
|---|
Principal Investigator |
NITTA Hiroshi Tokyo Medical and Dental University, Graduate School, associate professor, 大学院・医歯学総合研究科, 助教授 (70237767)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ODA Shigeru Tokyo Medical and Dental University, 大学院・医歯学総合研究科, 講師 (70160869)
NAGASAWA Toshiyuki Tokyo Medical and Dental University, 大学院・医歯学総合研究科, 助手 (90262203)
|
|---|
| Project Period (FY) |
2003 – 2005
|
| Project Status |
Completed (Fiscal Year 2005)
|
| Budget Amount *help |
¥14,800,000 (Direct Cost: ¥14,800,000)
Fiscal Year 2005: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2004: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 2003: ¥7,500,000 (Direct Cost: ¥7,500,000)
|
|---|
| Keywords | LPS / Periodontitis / IL-6 / CRP / エンドトキシン / LBP |
|---|
| Research Abstract |
1. Changes in levels of LPS and various inflammatory markers in blood by intervention of periodontal treatment.
Seventeen chronic periodontitis non-smoker patients without any systemic disease were subjected for the study. Levels of plasma LPS, serum IL-6, CRP and LBP were determined before, immediately after and one month after the full mouth mechanical debridement.
The results as follows :
(1) LPS was detected in the plasma from 3 out of 17 patients.
(2) The mean serum IL-6 level was elevated immediately after treatment, and reduced to the baseline level one month after treatment. The mean serum LPB level was reduced immediately after treatment, and returned to the baseline level one month after treatment. The mean CRP level was not significantly different during the study. Serum CRP levels of 5 patients were reduced after one month treatment.
These results suggested that quantity of LPS come into the circulation from periodontal lesion was very small or LPS was immediately inactivated by LBP etc. However serum IL-6 level was elevated and LBP level was reduced immediately after treatment. Therefore bacteria or LPS from periodontal lesion affect systemically.
2. Response of whole blood to low dose LPS.
Blood samples obtained from seven healthy individuals were pretreated with or without 5pg/ml Aa LPS, followed by further stimulation with 1ng/ml Aa LPS. The concentrations of IL-1〓, IL-6, IL-10 and TNF-〓 in the culture supernatants were then determined.
Pretreatment with 5pg/ml Aa LPS significantly enhanced the production of IL-1〓 and IL-6 from whole blood when further induced by 1ng/ml LPS.
These results suggested that a low dose of bloodstream LPS found in periodontitis patients appears to be sufficient to have priming effect and may be capable of affecting the systemic responses of immune and inflammatory cells.
|
