| Project/Area Number |
15390643
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
KINOSHITA Atsuhiro Tokyo Medical and Dental University, Faculty of Dentistry, Professor, 歯学部, 教授 (10242207)
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| Co-Investigator(Kenkyū-buntansha) |
ODA Shigeru Tokyo Medical and Dental University, Graduate School, Lecturer, 大学院・医歯学総合研究科, 講師 (70160869)
KONDO Keiko Tokyo Medical and Dental University, Faculty of Dentistry, Lecturer, 歯学部, 講師 (20282759)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2003: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | bone morphogenetic protein / BMP / enamel matrix derivative / EMD / regeneration therapy / periodontal regeneration / periodontal ligament / animal study |
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| Research Abstract |
1) Recombinant human bone morphogenetic protein-2 (rhBMP-2) with polylactic acid-polyglycolic acid copolymer/gelatin sponge (PGS) carrier was applied on two-wall- and three-wall surgically created periodontal defects on adult dogs. The histometric results suggested that rhBMP-2 enhanced bone formation in three-wall-periodontal defects.
2) Horizontal circumferential periodontal defects were surgically created around the root of premolar bilaterally in six beagle dogs. An elastic ligature was placed tightly around the root at the base of the defect in order to inhibit coronal migration of cells from intact periodontal ligament tissue. Enamel matrix derivative (EMD) was applied in the experimental side and carrier alone was applied in the contra-lateral side as control. rhBMP-2/PGS was applied in the experimental and control sites. Twelve weeks after the surgery, histmetric analysis was performed. There were not statistically significant differences in the amount of cementum formation betw
… More
een the groups. The distance between the bottom of the defect and apical terminal of ankylosis was significantly greater in EMD group compared to control.
3) Adenovirus with core binding factor alpha-1 (Cbfa1) gene adsorbed to porous beta-tri-calcium phosphate block was applied on horizontal circumferential periodontal defects in adult dogs. As all blocks were exposed to oral cavity in healing period, it was impossible to evaluate the effect of Cbfa1 on periodontal regeneration in this study.
4) Experimental periodontitis was induced on the mesial side of maxillary canines in six adult dogs. Calcium phosphate cement (CPC) was injected in the defects. Non-grafted defects on the contralateral side served as controls. Twelve weeks after surgery, periodontal tissue healing was evaluated histometrically. New bone, new cementum, and new connective tissue attachment and adhesion were significantly enhanced in the experimental sites. CPC provided stable wound healing and enhanced periodontal regeneration in periodontal defects in dogs with experimental periodontitis. Less
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