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Molecular-biological analysis by DNA microarray for diagnosis of occlusal trauma

Research Project

Project/Area Number 15390646
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Periodontal dentistry
Research InstitutionOsaka University

Principal Investigator

KITAMURA Masahiro  OSAKA UNIVERSITY, DENTAL HOSPITAL, ASSISTANT PROFESSOR, 歯学部附属病院, 講師 (10243247)

Co-Investigator(Kenkyū-buntansha) MURAKAMI Shinya  OSAKA UNIVERSITY, GRADUATE SCHOOL OF DENTISTRY, PROFESSOR, 大学院・歯学研究科, 教授 (70239490)
YAMADA Satoru  OSAKA UNIVERSITY, DENTAL HOSPITAL, ASSISTANT PROFESSOR, 歯学部附属病院, 講師 (40359849)
NOZAKI Takenori  OSAKA UNIVERSITY, GRADUATE SCHOOL OF DENTISTRY, RESIDENT, 大学院・歯学研究科, 助手 (30263304)
SAHO Teruyuki  OSAKA UNIVERSITY, DENTAL HOSPITAL, RESIDENT, 歯学部附属病院, 助手 (10263295)
YANAGITA Manabu  OSAKA UNIVERSITY, GRADUATE SCHOOL OF DENTISTRY, RESIDENT, 大学院・歯学研究科, 助手 (80379081)
池澤 一彦  大阪大学, 歯学部附属病院, 講師 (80294114)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥14,600,000 (Direct Cost: ¥14,600,000)
Fiscal Year 2005: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2004: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2003: ¥9,300,000 (Direct Cost: ¥9,300,000)
KeywordsDNA microarray / Occlusal trauma / Periodontal diseases
Research Abstract

This study was designed to investigate the mechanisms involved occurrence of occlusal trauma and search the diagnostic makers of occlusal trauma by cDNA microarray and semi-measurable reverse transcription-polymerase chain reaction (RT-PCR). In the in vitro occlusal trauma model utilizing cell-stretcher (Scholertec NS-200), we detected up-regulation of the gene expressions in the stretched human periodontal ligament cell (HPDL), such as cyclooxygenase-2 (Cox-2), c-fos, integrin β-1, β-actin and adenosine deaminase genes. In another way, the genes associated with periodontal ligament, such as periodontal ligament associated protein-1 (PLAP-1) and periostin, did not showed the change of gene expressions by the mechanical stress. Furthermore, in order to clarify the diagnostic makers of occlusal trauma, we detected the mRNA of inflammatory cytokines and mediators by RT-PCR in the gingival tissues with occlusal trauma and without occlusal trauma collected by needle-punched biopsy methods. In result, IL-1β, IL-6, IL-8 and Cox2 mRNA expression were decreased and IFN-γ mRNA expression was elevated in the periodontal lesions decreased the occlusal trauma by occlusal adjustment. In another way, the mRNA of inflammatory cytokines and mediators detected in this study did showed no change or slight elevation of the expression in the periodontal lesions with occlusal trauma treated with no occlusal adjustment. Present results show that the mechanical stress promotes the various gene expressions associated with inflammatory reactions and bone destruction in HPDL and suggest that the inflammatory cytokines and mediators detected in this study, such as IL-1β, IL-6, IL-8 and Cox2 and IFN-γ, could be the potential diagnostic makers of occlusal trauma.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (2 results)

All 2006

All Journal Article (2 results)

  • [Journal Article] Regulation of PLAP-1 Expression in Periodontal Ligament Cell2006

    • Author(s)
      Satoru Yamada
    • Journal Title

      Journal of Dental Research 85・5

      Pages: 447-451

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Regulation of PLAP-1 Expression in Periodontal Ligament Cell2006

    • Author(s)
      Satoru Yamada
    • Journal Title

      Journal of Dental Research 85・(未定)

    • Related Report
      2005 Annual Research Report

URL: 

Published: 2003-04-01   Modified: 2016-04-21  

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