| Project/Area Number |
15404009
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Chemistry related to living body
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| Research Institution | The University of Electro-Communications |
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Principal Investigator |
NIWA Haruki The University of Electro-Communications, Faculty of Electro-communications, Professor, 電気通信学部, 教授 (20135297)
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| Co-Investigator(Kenkyū-buntansha) |
HIRANO Takashi The University of Electro-Communications, Faculty of Electro-communications, Associate Professor, 電気通信学部, 助教授 (20238380)
MAKI Shojiro The University of Electro-Communications, Faculty of Electro-communications, Assistant Professor, 電気通信学部, 助手 (20266349)
OHMIYA Yoshihiro National Institute of Advanced Industrial Science and Technology, Research Institute for Cell Engineering, Group Reader, 人間系特別研究体, グループ長 (20223951)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥13,200,000 (Direct Cost: ¥13,200,000)
Fiscal Year 2005: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2003: ¥6,400,000 (Direct Cost: ¥6,400,000)
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| Keywords | bioluminescence / luminous worm / Odotosyllis / luminous snail / Latia / liciferin / liciferase / 分離 / 構造決定 / 蛍光性 / 抽出 / 発光生物 / 精製 |
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| Research Abstract |
1)From the marine luminescent worm, Odontosyll is phosphorea collected off from Mission Bay, San Diego, U.S.A., the light producing substance, Odontosyll is luciferin was isolated under the guidance of luciferin-luciferase reaction. The bioluminescence spectrum (λmax 500nm) using the isolated luciferin and the crude luciferase was similar to that of live specimens. The UV spectrum was essentially identical with that reported previously by Shimomura. The molecular formula of the luciferin was determined from the high resolution ESI mass spectrum to be C_<15>H_8O_<12>S_3.
2)Exploration of luminescent marine organisms was performed at New Caledonia, some luminous worm and sea hare was found.
3)From the fresh-water limpet like snail, Latia neritoides collected at the Nugtunu stream near Mt. Pirongia, New Zealand, the luciferase was isolated under the guidance of luciferin-luciferase reaction. The detailed biochemical studies indicated that the active form of the luciferase was found to be a hexamer of N-linked glycoprotein monomer having the molecular mass 31kDa. The cloning of the luciferase gene was succeeded. However heterologous expression of the gene was not succeeded so far.
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