| Project/Area Number |
15406015
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | Ehime University |
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Principal Investigator |
TORII Motomi Ehime University, School of Medicine, Professor, 医学部, 教授 (20164072)
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| Co-Investigator(Kenkyū-buntansha) |
KANEKO Osamu Ehime University, School of Medicine, Associate Professor, 医学部, 助教授 (50325370)
坪井 敬文 愛媛大学, 無細胞生命科学工学センター, 教授 (00188616)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥13,400,000 (Direct Cost: ¥13,400,000)
Fiscal Year 2005: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2004: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2003: ¥5,600,000 (Direct Cost: ¥5,600,000)
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| Keywords | infectious disease / malaria / Plasmodium / マラリア / 熱帯熱マラリア / 赤血球結合蛋白 / 微生物 / 侵入 / 遺伝子 |
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| Research Abstract |
Erythrocyte binding proteins are potent malaria vaccine candidates and the expression of these proteins in the field isolates has to be evaluated before vaccine development based upon these proteins. The objective of this project is to analyze mRNA and protein expression for the erythrocyte binding proteins, encoded by multigene family EBL, RBL, and Clag/RhopH1, which are involved in the erythrocyte invasion process. We collected P.falciparum field isolates in Thailand and cultured for short period and enriched mature schizonts. Using oligonucleotide targeting members of each multigene family, RT-PCR was performed to detect transcripts level. However, by preliminal analysis of the polymorphism of these genes, we found these gene family members are highly diversified and carefull design of the oligonucleotide is required to distinguish each member, but identify individual members. We generated a panel of antisera against each members and analyzed the expression levels of the each member. For Clag/RhopH1, the region showing diversified each other among members shows also polymorphic among isolates, indicated that it is required to generate antisera which distinguish each members, but react all type of the allele.
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