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Basic and applied research for early diagnosis and early treatment of visceral leishmaniasis

Research Project

Project/Area Number 15406018
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field Parasitology (including Sanitary zoology)
Research InstitutionAichi Medical University School of Medicine

Principal Investigator

ITOH Makoto  Aichi Medical University School of Medicine, Department of Parasitology, Associate Professor, 医学部, 助教授 (90137117)

Co-Investigator(Kenkyū-buntansha) KIMURA Eisaku  Aichi Medical University School of Medicine, Department of Parasitology, Professor, 医学部, 教授 (70153187)
HASHIGUCHI Yoshihisa  Kochi University Medical School, Department of Parasitology, Professor, 医学部, 教授 (10037385)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥8,000,000 (Direct Cost: ¥8,000,000)
Fiscal Year 2005: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2004: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2003: ¥3,300,000 (Direct Cost: ¥3,300,000)
Keywordsleishmaniasis / Immunodiagnosis / urine / recombinant protein / Bangladesh / sandfly / international collaboration / GPS / DAT(直接凝集法) / 内臓リーシュマニア症 / 尿 / ELISA / derect agglutination / 国際研究者交流
Research Abstract

The aim of this study was to develop simple diagnostic tools for visceral leishmaniasis (VL) using urine samples for early diagnosis and early treatment of the disease.
(1) Production of recombinant antigen, rKRP42, for urine diagnosis
A DNA fragment coding a part of kinesine related protein, rKRP42 (Accession number : AB256033), was cloned from Leishmania donovani genome and a recombinant protein rKRP42 was obtained from the expression induced E.coli cells. Specificity of the urine ELISA was improved with the recombinant antigen, which is constantly supplied with constant quality.
(2) Development of a simple diagnostic tool for VL
We developed a simple method for diagnosis of VL. It detects antibodies in urine samples with anti-human IgG coated plate and colored L.donovani promastigote. Its sensitivity and specificity is almost same as urine ELISA. As this method doesn't need any equipment and the results can be judged by naked eyes, it is suitable to be used in endemic areas where no fac … More ilities is available.
(3) Application of the urine ELISA for early diagnosis
Among four villages surveyed in 2003, a village, Nobai Bot Tala, was selected for our survey because of its high positive rate. Fifteen subjects became kala azar during one year from March 2005. Among them, 13 subjects had been examined as positive with urine ELISA before their onset of the symptoms. Therefore, the urine ELISA was suggested as a good tool for early diagnosis of kala azar and this prevent severe illness caused by delayed treatment.
(4) Survey of a vector, Phlebotomus species
P.argentipes which is know as a vector of VL was found at the village by preliminary survey conducted in July 2005. Seventy-one sandflies were trapped in March 2006 and 80% of them were found to be P.argentipes. The sandflies were trapped at 10 out of 12 points surveyed, showing its wide distribution in the village.
(5) Mapping of houses in Nobai Bot Tala
Using a handheld device of GPS and satellite image served by Google Earth, all houses in the village were identified and numbered on the satellite image. Analysis for generation status of VL in the village for these years is now going on. Less

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (35 results)

All 2005 2004 Other

All Journal Article (25 results) Book (3 results) Publications (7 results)

  • [Journal Article] Atypical clinical variation in new world cutaneous leishmanisis : disseminated, erysipeloid, and recidiva cutis due to Leishmania (V.) panamensis.2005

    • Author(s)
      Carvopina M.C.
    • Journal Title

      Am. J. Trop. Med. Hyg. 73

      Pages: 281-284

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Species assignation of Leishmania from human and canine American tegumentrary leishmaniasis in north Argentina.2005

    • Author(s)
      Marco J.D.
    • Journal Title

      Am. J. Trop. Med. Hyg. 72

      Pages: 606-611

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] フィラリア症・リーシュマニア症診断キットの問題点2005

    • Author(s)
      伊藤 誠
    • Journal Title

      Clinical Parasitology 16

      Pages: 30-32

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] フィラリア症・リーシュマニア症診断キットの問題点2005

    • Author(s)
      伊藤 誠, 木村英作
    • Journal Title

      Clinical Parasitology 16-1

      Pages: 30-32

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Specific assignation of Leishmania from human and canine American tegumentary leishmasniasis cases by multilocus enzyme electrophoresis in north Argentina2005

    • Author(s)
      Marco J.D., Barosso P.A., Calvopina M., Kumazawa H., Furuya M., Korenaga M., Cajal S.P., Mora M.C., Rea M.M., Borda C.E., Basonbria M.A., Taranto N.J., Hashiguchi Y.
    • Journal Title

      Am.J.Trop.Med.Hyg. 72-5

      Pages: 606-611

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Atypical clinical variation in new world cutaneous leishmaniasis : disseminated, erysipeloid, and recidiva cutis due to Leishmania (V.) panamensis.2005

    • Author(s)
      Calvopina M.C., Gomez E.A., Uezato H., Kato H., Nonaka S., Hashiguchi Y.
    • Journal Title

      Am.J.Trop.Med.Hyg. 73-2

      Pages: 201-284

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Atypical clinical variants in New World cutaneous leishmaniasis : deisseminated, erysipeloid, and recidiva cutis due to Leishmania (V.) panamensis.2005

    • Author(s)
      Calvopina M.
    • Journal Title

      Am.J.Trop.Med.Hyg. 73

      Pages: 281-284

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Clinical trial of 20% Mat lippia (bucan) topical ointment for cutaneous leishmaniasis ; a preliminary trial.2005

    • Author(s)
      Soomro F.R.
    • Journal Title

      J.Pak.Assoc.Dermatol. 15

      Pages: 108-112

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Species assignation of Leishmania from human and canine American tegumentrary leishmaniasis in north Argentina.2005

    • Author(s)
      Marco J.D.
    • Journal Title

      Am.J.Trop.Med.Hyg. 72

      Pages: 606-611

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Molecular phylogenic relationships among seven Japanese species of Cercopithifilaria2005

    • Author(s)
      Agatsuma T.
    • Journal Title

      Parasitology International 54

      Pages: 195-199

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Differential effects of diethylcarbamazine, tetracycline and the combination on Brugia pahangi adult female in vitro2005

    • Author(s)
      Gunawardena N.K.
    • Journal Title

      Parasitology International 54

      Pages: 253-259

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 国際医学・医療協力と寄生虫症-リーシュマニア症を中心に2005

    • Author(s)
      橋口義久
    • Journal Title

      高知医会誌 10

      Pages: 5-15

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Direct agglutination test with urine samples for the diagnosis of visceral leishmaniasis2004

    • Author(s)
      Islam M.Z.
    • Journal Title

      Am. J. Trop. Med. Hyg. 70

      Pages: 78-82

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Direct agglutination test with urine samples for the diagnosis of visceral leishmaniasis.2004

    • Author(s)
      Islam M.Z., Itoh M., Mirza R., Armed I., Ekram A.R.M.S., Sarder A.H., Shamuzzaman S.M., Hashiguchi Y., Kimura E.
    • Journal Title

      Am.J.Trop.Med.Hyg. 70-1

      Pages: 78-82

    • NAID

      120001671583

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Detection of Leishmania donovani antigen in urine by antigen capture enzyme-linked immunosorbent assay.2004

    • Author(s)
      Islam M.Z., Itoh M., Ekram A.R.M.S., Islam M.A.U., Hussain S.M., Ali M., Takagi H., Hashiguchi Y., Kimura E.
    • Journal Title

      Studies on new and old world leishmaniases and their transmission, with particular reference to Ecuador, Argentina and Pakistan. (Hashiguchi Y., ed.)(Kyowa Printing & Co. Ltd.)

      Pages: 78-84

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Direct agglutination test with urine samples for the diagnosis of visceral leishmaniasis2004

    • Author(s)
      Islam MZ
    • Journal Title

      American Journal of Tropical Medicine and Hygiene 70

      Pages: 78-82

    • Related Report
      2004 Annual Research Report
  • [Journal Article] フィラリア症撲滅宣言、その後-スリランカからの報告2004

    • Author(s)
      木村英作
    • Journal Title

      医学の歩み 209

      Pages: 189-192

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Detection of Leishmania (Leishmania) donovani antigen in urine by antigen capture enzyme-linked immunosorbent assay2004

    • Author(s)
      Islam MZ
    • Journal Title

      Studies on new and old world leishmaniases and their transmis-sion, with particular reference to Ecuador, Argentina and Pakistan 7

      Pages: 78-84

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Overexpression of LaMDR2, a novel multidrug resistance ATP-binding cassette transporter, causes 5-fluorouracil resistance in Leishmania amazonensis2004

    • Author(s)
      Ken Katakura
    • Journal Title

      FEBS Letters 561

      Pages: 207-212

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Sequence variation of the Cytochrome b gene of various human pathogenic members of the genus Leishmania : A new approach for their identification2004

    • Author(s)
      Gloria Luyo-Acero
    • Journal Title

      Parasitology 128・5

      Pages: 483-594

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Detection and identification of Leishmania species within naturally infected sandfies in the Andean areas of Ecuador by polymerase chain reaction2004

    • Author(s)
      Hirotomo Kato
    • Journal Title

      Am.J.Trop.Med.Hyg (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Production of recombinant kinesin-related protein of Leishmania donovani and its application in the serodiganosis of visceral leishmaniasis

    • Author(s)
      Takagi H.
    • Journal Title

      Am. J. Trop. Med. Hyg. (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Confirmation of the elimination of lymphatic filariasis by means ofIgG4 enzyme-linked immunosorbent assay using urine samples in Yongjia, Zhejiang Province and Gaoan, Jiangxi Province, China

    • Author(s)
      Itoh M.
    • Journal Title

      Am. J. Trop. Med. Hyg. (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Production of recombinant kinesin-related protein of Leishmania donovani and its application in the serodiganosis of visceral leishmaniasis

    • Author(s)
      Takagi H., Islam M.Z., Itoh M., Islam M.A.U., Ekram A.R.M.S., Hussain S.M., Hashiguchi Y., Kimura E.
    • Journal Title

      Am.J.Trop.Med.Hyg. (In press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Confirmation of the elimination of lymphatic filariasis by means of IgG4 enzyme-linked immunosorbent assay using urine samples in Yongjia, Zhejiang Province and Gaoan, Jiangxi Province, China.

    • Author(s)
      Itoh M., Wu W., Sun D., Yao L., Li Z., Islam M.Z., Chen R., Zhang K., Wang F., Zhu S., Kimura E.
    • Journal Title

      Am.J.Trop.Med.Hyg. (In press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] Filariasis in Asia and Western Pacific Islands2005

    • Author(s)
      Kimura E.
    • Total Pages
      276
    • Publisher
      Editors : Yano A (Chief Editor), Kimura E, Rim HJ, Sun DJ, Weerasooriya WA
    • Related Report
      2005 Annual Research Report
  • [Book] Filariasis in Asia and Western Pacific Islands (Editors : Yano A (Chief Editor), Kimura E, Rim HJ, Sun DJ, Weerasooriva WA)2005

    • Author(s)
      Kimura E
    • Total Pages
      300
    • Publisher
      (in press)
    • Related Report
      2004 Annual Research Report
  • [Book] Studies on new and old world leishmaniases and their transmission, with particular reference to Ecuador, Argentina and Pakistan2004

    • Author(s)
      Islam MZ
    • Total Pages
      272
    • Publisher
      Detection of Leishmania (Leishmania) donovani antigen in urine by antigen capture enzyme-linked immunosorbent assay
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Publications] Islam, M.Z.: "Direct agglutination test with urine samples for the diagnosis of visceral leishmaniasis"American Journal of Tropical Medicine an Hygiene. 70・1. 78-82 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Itoh, M.: "Sensitive enzyme-linked immunosorbent assay with urine samples : a tool for surveillance of schistosomiasis japonica"Southeast Asian Journal of Tropical Medicine and Public Health. 34・3. 469-472 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Weerasooriya, M.V.: "Human infection with Wuchereria bancrofti in Matara, Sri Lanka : the use, in parallel of an ELISA to detect filaria-specific IgG4 in urine and of ICT card tests to detect filaria] antigen in whole blood"Annals of Tropical Medicine and Parasitology. 97・2. 179-189 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Watanabe, K.: "Bancroftian filariasis in Nepal : a survey for circulating antigenemia of Wuchereria bancrofti and urinary IgG4 antibody in two areas of Nepal"Acta Tropica. 88. 11-15 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Weerasooriya, M.V.: "Prevalence and levels of filaria-specific urinary IgG4 among children less than five years of age and the association of antibody positivity between children and their mothers"American Journal of Tropical Medicine and Hygiene. 68・4. 465-468 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yoshihisa Hashiguchi: "Leishmaniasis"Progress of Medical Parasitology in Japan.. 7. 537-553 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 橋口 義久: "医学大辞典(小児リーシュマニア他)"医学書院. 17 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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