| Project/Area Number |
15406028
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Hygiene
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
KOBAYASHI Nobumichi Sapporo Medical University, School of Medicine, professor, 医学部, 教授 (80186759)
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| Co-Investigator(Kenkyū-buntansha) |
KOJIMA Kazunobu Sapporo Medical University, School of Medicine, assistant professor, 医学部, 講師 (20264517)
ISHINO Masaho Sapporo Medical University, School of Medicine, associate professor, 医学部, 助教授 (30232325)
TANIGUCHI Koki Fujita Health University, School of Medicine, professor, 医学部, 教授 (40094213)
MISE Keiji Sapporo Medical University, School of Medicine, instructor, 医学部, 助手 (30200025)
SUMI Ayako Sapporo Medical University, School of Medicine, assistant professor, 医学部, 講師 (10363699)
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| Project Period (FY) |
2003 – 2005
|
| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥11,400,000 (Direct Cost: ¥11,400,000)
Fiscal Year 2005: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2004: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2003: ¥5,500,000 (Direct Cost: ¥5,500,000)
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| Keywords | rotavirus / diarrhea / adult / Southern Asia / Southeast Asia / typing / gene / sequence / 下痢 / アジア / G型 / P型 / NSP4 |
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| Research Abstract |
The purpose of this study is to characterize rotaviruses which cause diarrheal diseases in adults in Southern Asian and Southeast Asian regions. This study was performed in collaboration with India, Bangladesh, Thailand, and China. Stool specimens were collected from diarrheal patients in a period between 2003 and 2005. While detection rate of group A rotavirus in children was 25-35%, detection rate in adult ranged from approx. 1% to 10%. In the study in China, most rotavirus strains from children and adults showed identical antigenic types. Group B rotavirus was detected in adult stool specimens in Bangladesh (14 specimens) and China (2 specimens). In India, group B rotavirus was detected by RT-PCR in about 20% of diarrheal stool specimens from children. Phylogenetic analysis of the group B rotaviruses from individual countries indicated that the viruses from India and Bangladesh were genetically closely related but distinct from Chinese viruses. The Indian-Bangladeshi group B rotavir
… More
uses and Chinese viruses were suggested to be diverged from a common ancestral virus approximately 60-70 years ago based on estimation of mutation rate calculated for Chinese virus strains. In Bangladesh, an unusual rotavirus strain was detected. This virus was genetically different from the known group A, B, and C rotaviruses, and suggested to represent a novel species (group) of rotavirus. As a study on special characteristic of group B rotavirus, i.e., causing diarrhea primarily in adults, nonstructural protein NSP4 was studied for its sequence diversity and presence of enterotoxin activity which is known as one of the mechanisms causing diarrhea in animals. As a result, group B rotavirus NSP4 exhibited high degree of sequence divergence from group A rotavirus NSP4, however, it was demonstrated that sequences near the C-terminal region possess enterotoxin activity in suckling mice. These findings suggested that group B rotavirus may have similar mechanisms for causing diarrhea in human to group A rotavirus. Less
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