| Project/Area Number |
15500196
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Bioinformatics/Life informatics
|
|---|
| Research Institution | Tokyo Medical and Dental University |
|---|
Principal Investigator |
KUBOTA Michinori Tokyo Medical and Dental University, Medical Research Institute, associate professor, 難治疾患研究所, 助教授 (30186457)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HOSOKAWA Yutaka Tokyo Medical and Dental University, Medical Research Institute, assistant professor, 難治疾患研究所, 助手 (80181501)
|
|---|
| Project Period (FY) |
2003 – 2004
|
| Project Status |
Completed (Fiscal Year 2004)
|
| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2004: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2003: ¥2,500,000 (Direct Cost: ¥2,500,000)
|
|---|
| Keywords | Optical imaging / Spatio-temporal patterns / Voltage-sensitive dye / Auditory cortex / Slice preparation |
|---|
| Research Abstract |
Optical imaging was conducted in rat auditory cortex slice preparations to study spatiotemporal patterns of response to repetitive stimulation in the different layers using voltage sensitive dye (di-4-ANEPPS). Rats were anesthetized with isoflurene. Brains were rapidly removed and frontal slices were cut at 400 μm. The image of the slice was focused on a 90×60 pixel high-speed CCD camera and optical signals were recorded every 1.0 ms. Electrical stimulation of the infragranular layers elicited horizontal responses both in the supragranular and infragranular layers. When the rate of repetitive stimulation increased to 40 Hz, the amplitude ratio of the fifth response elicited by the repetitive stimulation pulse to the first one was significantly smaller in layers II/III than that elicited in layer IV and infragranular layers. The similar results were obtained when electrical stimulation was applied to the other layers (layer II/III or layer IV), although spatiotemporal patterns of response were different from the ones elicited by the stimuli in the infragranular layers. There was no difference in responses among layers when the rate of stimulation was at 10 Hz. These results suggest that the way of response to repetitive stimulation is different among different layers in the auditory cortex, especially between supragranular and infragranular layers and is stimulation rate-dependent.
|
