| Project/Area Number |
15500219
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
INOUE Masashi Tokyo University of Pharmacy and Life Science, Department of Life Science, Assistant Professor, 生命科学部, 助手 (30339098)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAKAWA Hiroyoshi Tokyo University of Pharmacy and Life Science, Department of Life Science, Associate Professor, 生命科学部, 助教授 (90166124)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2004: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2003: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | Ca^<2+> imaging / Oregon Green 488 BAPTA-1 / Spike synchrony / Rat / Hippocampal slice / Burst / Granule cell / Mossy cell / 顆粒細胞 / カルシウム・イメージング / ラット |
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| Research Abstract |
Monitoring neuronal activities from multiple neurons is essential for understanding neuronal network activities. While calcium imaging from a population of cells is an effective method to study the network dynamics of a neural structure, it has been difficult to image from densely packed structures, such as the granule cell layer of the dentate gyrus, due to overlap of the cells. We have developed a novel method to label multiple granule cells with a Ca^<2+> indicator in rat hippocampal slices using Oregon Green 488 BAPTA-1 AM. Synchronized burst activities (0.3-1.4 Hz), which were induced by applying 50 μM 4-aminopyridine, were monitored extracellularly with a glass electrode placed at the granule cell layer in the dentate gyrus. During the burst activities, spontaneous occurring action potential-induced Ca^<2+> transients from multiple (4-12) granule cells were monitored with a cooled CCD camera with single-cell resolution. Temporal structures of firing patterns from the multiple neurons were determined from Ca^<2+> transients. In each single burst event recorded from the extracellular electrode, each neuron fired synchronously within a 200 ms time window. The latency and its variance from the onset time of the single burst events to one of the Ca^<2+> transients were decreased over time (<7.5 min). These results indicate that the synchrony of the action potentials within a single burst event was enhanced as the burst activities proceeded. The progressive synchronization may be a key feature to make self-organizing network activities.
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