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Mutual regulatory mechanism between Ca2+ permeable receptors and channels and Ca2+ binding proteins

Research Project

Project/Area Number 15500223
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionTokyo Metropolitan Organization for Medical Research

Principal Investigator

OKADO Haruo  Tokyo Metropolitan Organization for Medical Research, Tokyo Metropolitan Institute for neuroscience, Staff Sceintist, 東京都神経科学総合研究所, 副参事研究員 (60221842)

Co-Investigator(Kenkyū-buntansha) MIWA Akiko  Tokyo Metropolitan Organization for Medical Research, Tokyo Metropolitan Institute for neuroscience, Staff Sceintist, 東京都神経科学総合研究所, 研究員 (60142155)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsGlutamate receptor / calbindinD28k / AMPA receptor / CREB / calcium binding protein / カルシウム透過型AMPA受容体 / CalbindinD28k / NMDA受容体 / カルシウム結合蛋白 / paravalbumin / AMPA受容体 / カルビンジン / CREB
Research Abstract

We reported that the expression of Ca2+ binding proteins, calbindin-D28k (CB) and parvalbumin correlates with the expression of Ca2+ permeable AMPA receptors (AMPA-Rs) in rat forebrain. We analyzed cell surface expression of a Ca2+ impermeable subunit, GluR2 and a Ca2+ permeable subunit, GluR2Q using an adenoviral-mediated expression system in COS7 cells. Biochemical measurements of cell surface expressed subunits using biotinylation experiments revealed that cell surface expression of GluR2Q is significantly increased with CB coexpression. By application of AMPA-R blockers, cell surface expression of GluR2Q is increased in the absence of CB coexpression. To determine whether Ca2+ entry via Ca2+ permeable AMPA receptor causes the suppression, we used the Ca2+ chelater, BAPTA-AM. Application of BAPTA-AM increases cell surface expression of GluR2Q. These results suggest that CB enhances cell surface expression of Ca2+ permeable AMPA-Rs by buffering the increased intracellular Ca2+ that enters through Ca2+ permeable AMPA-Rs.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (7 results)

All 2004 Other

All Journal Article (6 results) Publications (1 results)

  • [Journal Article] Calbindin-D28k coexpression enhances cell surface expression of da2+ permeable AMPA receptors2004

    • Author(s)
      Takahashi A, Kondo M, Miwa A, Okado H
    • Journal Title

      Japanese Journal of Physiolog 54

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Calbindin-D28k coexpression enhances cell surface expression of Ca2+ permeable AMPA receptors2004

    • Author(s)
      Takahashi A, Kondo M, Miwa A, Okado H
    • Journal Title

      Japanese Journal of Physiolog 54

    • NAID

      130005446929

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The subfomical organ is the primary locus of sodium-level sensing by Nax sodium channels for the control of salt-intake behavior.2004

    • Author(s)
      Hiyama TY, Watanabe E, Okado H, Noda M.
    • Journal Title

      J Neurosci. 24(42)

      Pages: 9276-9281

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Newly-formed axonal branches of rat sciatic neurons sprouting in the spinal cord after peripheral axotomy.2004

    • Author(s)
      Zenzai K, Shibata M, Okado H, Endo N, Hirano S.
    • Journal Title

      Scand J Plast Reconstr Surg Hand Surg 38(3)

      Pages: 192-234

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Distinct aggregation and cell death patterns among different types of primary neurons induced by mutant huntingtin protein.2004

    • Author(s)
      Tagawa K, Hoshino M, Okuda T, Ueda H, Hayashi H, Engemann S, Okado H, Ichikawa M, Wanker EE, Okazawa H.
    • Journal Title

      J Neurochem. 89(4)

      Pages: 974-987

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Sequential exocytosis of insulin granules is associated with redistribution of SNAP25.2004

    • Author(s)
      Takahashi N, Hatakeyama H, Okado H, Miwa A, Kishimoto T, Kojima T, Kojima T, Abe T, Kasai H.
    • Journal Title

      J Cell Biol. 165(2)

      Pages: 255-262

    • Related Report
      2004 Annual Research Report
  • [Publications] Saito et al.: "Transfer of NMDAR2 cDNAs increases endogenous NMDAR1 protein and induces expression of functional NMDA receptors in PC12 cells."Brain Res Mol Brain Res.. 110. 159-168 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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