| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2004: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Research Abstract |
We have evaluated postischemic neurological and cognitive dysfunction after mild focal cerebral ischemia in Mongolian gerbil. Mongolian gerbils underwent the left common carotid artery occlusion (CCAO) for 10, 15, or 20 min. A battery of neurological tests including T maze test, bilateral asymmetry test, open field test, elevated body swing test and methamphetamine (MP)-induced rotation test was performed to evaluate the neurological and cognitive dysfunction including f extrapyramidal motor dysfunction.
1.Postischemic motor and somatosensory functions were significantly correlated with regional infarction volumes in the corresponding cortical regions. In gerbils, visual abnormality could be independently detected by the T-maze test. Such regional analyses of ischemic lesions would be useful for investigating the functional outcomes of stroke therapy.
2.Biased rotation behavior is a sensitive parameter of the extent of striatal injury after focal cerebral ischemia provided the lesion is
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not extended to the ipsilateral cortex. UP-induced rotation in rodents probably coordinates with the extrapyramidal motor dysfunction after striatal infarction in patients with vascular Parkinsonism.
3.The total infarction volumein the neuronal stem cell -grafted animals was significantly lower than that in controls. Approximately 8% of the grafted stem cell urvived, mainly in areas of selective neuronal death, and were costained with antibodies against neuronal nuclei antibody, microtubule associated protein, glial fibrillary acidic protein. Synaptic structures between stem cell derived neurons and host neurons were bserved. Furthermore, gradual improvement of neurologic functions was observed clearly in the stem cell grafted animals, compared to that in controls. Human neuronal stem cell remarkably improved neurologic functions after focal ischemia in the Mongolian gerbil, and maintained their abilities to migrate around the infarction, differentiate into mature neurons, and form synapses with host neuronal circuits. Less
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