• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

An evaluation of anti-VacA antibody and its target molecules in the cerebrospinal fluid of patients with demyelinating form of Guillain-Barre syndrome.

Research Project

Project/Area Number 15500242
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionSapporo Medical University

Principal Investigator

CHIBA Susumu  Sapporo Medical University, Department of Neurology, School of Medicine, Associate Professor, 医学部, 助教授 (30167514)

Co-Investigator(Kenkyū-buntansha) SUGIYAMA Toshiroh  Toyama University, The 3rd Department of Internal Medicine, School of Medicine, Professor, 医学部, 教授 (00196768)
IMAI Tomihiro  Sapporo Medical University, Department of Neurology, School of Medicine, Assistant Professor, 医学部, 講師 (40231162)
NONAKA Michio  Sapporo Medical University, Department of Neurology, School of Medicine, Instructor, 医学部, 助手 (50363685)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsHelicobacter pylori / anti-VacA antibody / cerebrospinal fluid / Guillain-Barre syndrome / molecular homology / 分子相同性 / 髄液抗HSP抗体 / 自然免疫応答 / 免疫組織化学 / 末梢神経・神経根 / Na^+-K^+ ATPaseαsubunit / ミエリンPO蛋白
Research Abstract

Immunological study
In order to detect antibodies against recombinant vacuolating cytotoxin (r-VacA) of Helicobacter pylori (H.pylori) in the cerebrospinal fluid (CSF) from patients with Guillain-Barre syndrome (GBS). The CSF samples from 13 patients with GBS (electrophysiologically classified as 8 acute inflammatory demyelinating polyradiculoneuropathy (AIDP), 4 acute motor axonal neuropathy (AMAN), and 1 unexcitable) and 8 disease control patients were studied. The r-VacA protein was separated by SDS-PAGE and Western blot analysis was carried out. Six of the 13 GBS patients had a specific IgG antibody against VacA of H.pylori, which was confirmed by absorption experiments using r-VacA. Every patient with positive CSF anti-r-VacA antibody had AIDP. The sequence homology between VacA and human (Na^++K^+)-ATPase α subunit has been outlined. Our findings lead to the possible hypothesis that anti-VacA antibodies involve ion channels in abaxonal Schwann cell plasmalemma resulting in demyeli … More nation in some patients with GBS.
Histochemical evaluation
To identify the target molecules of anti-VacA-antibody (A-VacA-Ab) in the human ventral nerve roots, the immunohistochemical examination was carried out. A-VacA-Ab obtained from rabbits was prepared by immunization of the native VacA purified by the culture media of H.pylori. Cervical ventral nerve roots were obtained from patients with no known neurologic disorders within two hours after death. Both DAB and FITC procedures were employed. Disseminated spotty stainings were observed in cross sections. In longitudinal sections, string-like immunostainings were also seen both by DAB and FITC procedures. These were localized within the nerve fibers. Consequently, the target epitopes of A-VacA-Ab were thought to be present in the myelin components of the nerve roots. These results indicate that the target molecules of A-VacA-Ab in the cerebrospinal fluid obtained from patients with GBS are probably associated with some components of the peripheral nerve myelin. A-VacA-Ab may play an important role in the immune responses of patients with the demyelinating form of GBS. Less

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (18 results)

All 2006 2005 2004 2003 Other

All Journal Article (15 results) Publications (3 results)

  • [Journal Article] Autoantibodies against HSP7O family proteins in CSF of multiple sclerosis; :.2006

    • Author(s)
      Chiba S, Yonekura K, Yokota S, Tanaka S, Fujii N, Matsumoto H.
    • Journal Title

      J Neurol Sci 241

      Pages: 39-43

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Autoantibodies against HSP70 family proteins in CSF of multiple sclerosis.2006

    • Author(s)
      Chiba S, Yonekura K, Yokota S, Tanaka S, Fujii N, Matsumoto H.
    • Journal Title

      J Neurol Sci 241

      Pages: 39-43

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] An evaluation of immunological cross-reactvity between Vac-A of Helicobacter pylori and myelin P0 protein using the cerebrospinal fluid from patients with Guillain-Barre syndrome.2005

    • Author(s)
      Chiba S, Yonekura K, Yokota S, Tanaka S, Furuyama H, Okabayashi T, Matsumoto H, Fujii N.
    • Journal Title

      Helicobacter 10

      Pages: 514-514

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] NAD-dependent histone deacetylase SIRT (Sir 2α).2005

    • Author(s)
      Hisahara S, Chiba S, Matsumoto H, Horio Y
    • Journal Title

      J Pharmacologicol Sci 98

      Pages: 200-204

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Autoantibodies against HSP70 family proteins in CSF of multiple sclerosis.2005

    • Author(s)
      Chiba S, Yonekura K, Yokota S, Tanaka S, Fujii N, Matsumoto H
    • Journal Title

      J Neurol Sci 241

      Pages: 39-43

    • Related Report
      2005 Annual Research Report
  • [Journal Article] An evaluation of immunological cross-reactvity between Vac-A of Helicobacter pylori and myelin P0 protein using the cerebrospinal fluid from patients with Guillain-Barre syndrome2005

    • Author(s)
      Chiba S, Yonekura K, Yokota S, Tanaka S, Furuyama H, Okabayashi T, Matsumoto H, Fujii N.
    • Journal Title

      Helicobacter 10

      Pages: 514-514

    • Related Report
      2005 Annual Research Report
  • [Journal Article] An antibody to VacA of Helicobacter pylori in the CSF of patients with Miller-Fisher syndrome.2004

    • Author(s)
      Chiba S, Sugiyama T, Yonekura K, Tanaka S, Matsumoto H, Fujii N, Yokota S, Hirayama T.
    • Journal Title

      Neurology 63

      Pages: 2184-2187

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Prevalence of anti-heat shock protein in cerebrospinal fluids of patients with Guillain-Barre syndrome.2004

    • Author(s)
      Yonekura K, Yokota S, Tanaka S, Kubota H, Fujii N, Matsumoto H, Chiba S.
    • Journal Title

      J Neuroimmunol 156

      Pages: 204-209

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Localization and function in ER stress tolelance of Erdj3, a new member of HSP4O family protein.2004

    • Author(s)
      Nakanishi K, Kamiguchi K, Torigoe T, Nabeta C, Hirohashi Y, Asanuma H, Tobioka H, Koge N, Harad IO, Ichimiya S, Noagno H, Yano S, Chiba S, Matsumoto H, Sato N.
    • Journal Title

      Cell stress and Chaperone 9

      Pages: 253-264

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 中毒性・薬剤性ニューロパチー2004

    • Author(s)
      千葉 進, 田中真悟, 古山裕康
    • Journal Title

      Medicina 41

      Pages: 1329-1331

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Advanced Hirayama disease with successful improvement of activities of daily living by operative reconstruction.2004

    • Author(s)
      Chiba S, Yonekura K, Nonaka M, Imai T, Matumoto H, Wada T_1
    • Journal Title

      Int Med 43

      Pages: 79-81

    • NAID

      130000083978

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Localization and function in ER stress tolelance of Erdj3, a new member of HSP40 family protein.2004

    • Author(s)
      Nakanishi K, Kamiguchi K, Torigoe T, Nabeta C, Hirohashi Y, Asanuma H, Tobioka H, Koge N, Harad IO, Ichimiya S, Noagno H, Yano S, Chiba S, Matsumoto H, Sato N.
    • Journal Title

      Cell stress and Chaperone 9

      Pages: 253-264

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Helicobacter pylori 感染と神経疾患.2003

    • Author(s)
      千葉 進, 田中真悟
    • Journal Title

      Helicobacter Research 7

      Pages: 327-331

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Structures, biological activities and antigenic properties of Helicobacter pylori lipopolysaccharide.2003

    • Author(s)
      Yokota S, Amano K, Chiba S, Fujii N.
    • Journal Title

      Recent Reserch Developments in Microbiology 7

      Pages: 251-267

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Antibodies against VacA of Helicobacter pylon can recognize the epitopes of the human peripheral nerve myelin protein.2003

    • Author(s)
      Chiba S, Sugiyama T, Matsumoto H, Yonekura K, Tanaka S et al.
    • Journal Title

      Helicobacter 8(4)

      Pages: 445-445

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Publications] 千葉 進, 田中真悟: "Helicobacter pylori感染と神経疾患"Helicobacter Research. 7. 327-331 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yokota S, Amano K, Chiba S, Fujii N: "Structures, biological activities and antigenic properties of Helicobacter pylori lipopolysaccharide"Recent Reserch Developments in Microbiology. 7. 251-267 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Chiba S, Sugiyama T, Matsumoto H, Yonekura K, Tanaka S, et al.: "Antibodies against VacA of Helicobacter pylori can recognize the epitopes of the human peripheral nerve myelin protein"Helicobacter. 8(4). 445 (2003)

    • Related Report
      2003 Annual Research Report

URL: 

Published: 2003-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi