| Project/Area Number |
15500248
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Tokyo Metropolitan Organization for Medical Research |
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Principal Investigator |
ISHIZUKA Norio Tokyo Metropolitan Organization for Medical Research, Tokyo Metropolitan Institute for Neuroscience, 東京都神経科学総合研究所, 参事研究員 (90126201)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Memory / Hippocampus / Rat / PHA-L / Presubiculum / Associational projection / Auditory cortex / 嗅内野 / 局在投射 |
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| Research Abstract |
Organization of associational and commissural connections in the rat presubiculum was studied by anterograde labeling with Phaseolus vulgaris leucoagglutinin and retrograde labeling with horseradish peroxidase conjugated to wheat germ agglutinin. To facilitate the analysis, the hippocampal formation was "flattened" which allowed sections to be cut perpendicular to the full septotemporal axis of the presubiculum. Majority of the associational and commissural cells of the presubiculum were distributed in layer II and fewer in layer V, while hardly any labeled cells were observed in layers III and VI.
Layer II cells gave rise to bilateral projection to layers II and V and deep part of layer I of the preseubiculum. On the other hand, layer V cells gave rise to almost ipsilateral associational projections largely to layer V and fewer to layer II. As to the longitudinal (septotemporal) connection, layer II cells located in the septal presubiculum gave rise to projections to the temporal part. These projection neurons were densely distributed in the distal (far from the subiculum) part and fewer in the proximal (near to the subiculum) part, but very sparsely distributed in the middle region. Layer II cells in the temporal presubiculum also gave rise to many projection fibers to the septal presubiculum, predominantly to the distal part, but very few of them gave rise to projections to the proximal part. Notably, layer II projection and commissural neurons were almost absent in a band-like zone about 100μm from the proximal edge (the border between the presubiculum and the subiculum) through the full longitudinal extent. In layer V, longitudinal association neurons and their terminals distributed in the whole proximodistal extent of the presubiculum. Layer V cells in the septal presubiculum gave rise to projections mostly to the septal part and those in the temporal part to the temporal presubiculum.
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