| Project/Area Number |
15500254
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Kansai Medical University (2004)
Niigata University (2003) |
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Principal Investigator |
OKADA Masayoshi Kansai Medical University, Faculty of Medicine, Assistant professor, 医学部, 講師 (40334677)
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| Co-Investigator(Kenkyū-buntansha) |
樋口 宗史 新潟大学, 大学院・医歯学総合研究科, 教授 (30150337)
山口 剛 新潟大学, 大学院・医歯学総合研究科, 助手 (70323970)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2004: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2003: ¥600,000 (Direct Cost: ¥600,000)
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| Keywords | T-type Ca^<2+>_channel / neuropeptideY / patch-clamp technique / RT-PCR / NG108-15 cell / hippocampal slice / ラット海馬 / スライス培養 / スライスパッチクランプ / 電位依存性電流 / ホールセル記録 / NPY受容体 |
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| Research Abstract |
T-type Ca^<2+> channel plays pivotal roles in regulating the excitability of the neurons and non-neuronal excitable cells. The neuropeptideY (NPY) is one of the most abundantly expressed neuropeptides in the central and peripheral nervous systems, and its distribution correlates well with that of T-type Ca^<2+> channel, suggesting a functional interaction with it. The aim of this study was to investigate the functional interaction. In the former half of this study, I used the undifferentiated NG108-15 cells, in which the expression of the T-type Ca^<2+> channel current was confirmed. I found that low doses of NPY significantly augmented the T-type Ca^<2+> channel current with whole cell patch-clamp technique using Ba^<2+> as the charge carrier. The NPY treatment shifted the I-V relationship negatively. Experiments using NPY receptor subtype-selective agonist/antagonists revealed that both Y_1 and Y_2 receptors additively involved in the augmentation of the current. Consistently, RT-PCR analysis showed the expressions of mRNAs for Y_1 and Y_2 receptors. In the later half, to investigate the functional interaction, which was shown in the NG 108-15 cells, in the brain preparation, I set up culture system of hippocampal slice, which was reported to express the proteins. I examined the voltage-dependent channel currents expressed in the CA1 pyramidal neurons with slice patch-clamp technique. Further work is needed to obtain the conclusion of the NPY's effect on the voltage-dependent channel currents.
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