Regulation of channel activities by glycosylation during acquirement of neural plasticity

• Project/Area Number: 15500260
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Osaka Prefecture University (2004); Nara Institute of Science and Technology (2003)
• Principal Investigator: KATO Keiko Osaka Prefecture University, Graduate School of Life and Environmental Sciences, Associate professor, 大学院・農学生命科学研究科, 助教授 (90252684)
• Project Period (FY): 2003 – 2004
• Project Status: Completed (Fiscal Year 2004)
• Budget Amount: ¥3,700,000 (Direct Cost: ¥3,700,000); Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000); Fiscal Year 2003: ¥1,900,000 (Direct Cost: ¥1,900,000)
• Keywords: sialylation / neural plasticity / raft / glycosyltransferase / channel / epilepsy / knock-out

Research Abstract

I have proposed a hypothesis that diversity of neural plasticity is regulated by post-translational modification of proteins. I have focused on sialylated glycoproteins in brain and have investigated on sialylation in neural plasticity in vivo and in vitro. We have found that kindling stimulation of mice induced acquirement of structural neural plasticity and the acquirement induced increase of a α2,3-sialyltransferase expressions in the hippocampus and the anterior thalamic nuclei which receive projects from the hippocampus. As the α2,3-sialyltransferase is a key enzyme for sialylation of several channels, we planed to investigate on effects of the enzyme on how channel functions are correlated with changes of structural neural plasticity. First, we prepared conditional knock-out mice against the enzyme gene in this grand. During two years, we finished to make the model mice. At the same time, second, we planed to examine membrane dynamics between rafts and synapses for understanding effects of sialylation on channel functions. During this grand, we determined conditions to separate from raft to synapse membranes and we found different glycan forms on several channels between raft and synapse membranes. This grand gave a chance that I start to investigate membrane dynamics of channels in α2,3-sialyltransferase deficient mouse brain.

Research Products

• [Journal Article] 小脳グリア細胞の分化機構-糖鎖生物学の視点から- (2005)
  • Author(s): 加藤啓子, 平林義雄
  • Journal Title: CLINICAL NEUROSCIENCE(臨床神経科学) 23 Pages: 144-147
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Development of the cerebellar glial cells. (2005)
  • Author(s): Kato K, Hirabayashi Y.
  • Journal Title: Clinical Neuroscience 23 Pages: 144-147
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Region-specific and epileptogenic-dependent expression of six subtypes of α2,3-sialyltransferase in the adult mouse (2004)
  • Author(s): Matsuhashi, H., Horii Y., Kato, K.
  • Journal Title: J Neurochem 84 Pages: 53-66
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] てんかんモデルマウスにおける糖鎖修飾 (2004)
  • Author(s): 加藤啓子
  • Journal Title: 「蛋白質 核酸 酵素」増刊号神経糖鎖生物学 49 Pages: 2491-2498
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] 神経可塑性の獲得機構における分子生物学的基盤の解明 (2004)
  • Author(s): 加藤啓子
  • Journal Title: 三共生命科学研究振興財団 研究報告集 20 Pages: 54-65
  • NAID: 40006478057
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Differential sialylation on kindling model mouse. (2004)
  • Author(s): Kato K.
  • Journal Title: Protein, Nucleic Acid and Enzyme 49 Pages: 2491-2498
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Region-specific and epiletogenic-dependent expression of six subtypes of α2,3-sialyltransferase in the adult mouse (2004)
  • Author(s): Matsuhashi, H., Horii Y., Kato, K.
  • Journal Title: J Neurochem 84 Pages: 53-66
• [Journal Article] Region-specific and epileptogenic-dependent expression of six subtypes of α2,3-sialyltransferase in the adult mouse brain. (2003)
  • Author(s): Matsuhashi, H., Horii Y., Kato, K.
  • Journal Title: J Neurochem 84 Pages: 53-66
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Distribution of serine proteinase inhibitor, clade B, member 6 (Serpinb6) in the adult mouse brain. (2002)
  • Author(s): Kishi T., Matsuhashi H., Bird, P.I., Kato, K.
  • Journal Title: Gene Expression Patterns 1 Pages: 175-180
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Role of loop structures of neuropsin in activity of serine protease and regulated secretion. (2002)
  • Author(s): Oka T., Hakoshima, T., Itakura, M., Yamamori, S., Takahashi, M., Hashimoto, Y., Shiosaka S., Kato, K.
  • Journal Title: J Biol Chem 277 Pages: 14724-14730
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Extracellular serine protease neuropsin (KLK8) modulates neurite outgrowth and fasciculation of mouse hippocampal neurons in culture. (2002)
  • Author(s): Oka, T., Akisada, M., Okabe, A., Sakurai, K., Shiosaka, S., Kato, K.
  • Journal Title: Neurosci.Lett 321 Pages: 141-144
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Serine proteinase inhibitor 3 and murinoglobulin I are potent inhibitors of neuropsin in adult mouse brain. (2001)
  • Author(s): Kato, K., Kishi, T., Kamachi T., Akisada, M., Oka, T., Midorikawa, R., Takio, K., Dohmae, N., Bird, P.I., Sun, J., Scott, F., Miyake, Y., Yamamoto, K., Machida, A., Tanaka, T., Matsumoto, K., Shibata, M., Shiosaka, S.
  • Journal Title: J Biol Chem 276 Pages: 14562-14571
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Differential expression of mRNAs for sialyltransferase isoenzymes induced in the hippocampus of mouse following kindled-seizures. (2001)
  • Author(s): Okabe, A., Tawara, Y., Masa, T., Oka, T., Machida, A., Tanaka, T., Matsuhashi, H., Shiosaka, S., Kato, K.
  • Journal Title: J Neurochem 77 Pages: 1-14
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Dendritic aberrations in the hippocampal granular layer and the amygdalohippocampal area following kindled-seizures. (2001)
  • Author(s): Kato, K., Masa, T., Tawara, Y., Kobayashi, K., Oka, T., Okabe, A., Shiosaka, S.
  • Journal Title: Brain Res 901 Pages: 281-295
  • Description: 「研究成果報告書概要(欧文)」より
• [Patent(Industrial Property Rights)] 脳内物質の回収装置 (2003)
  • Inventor(s): 加藤 啓子
  • Industrial Property Rights Holder: 奈良先端科学技術大学院大学
  • Industrial Property Number: 2003-380832
  • Filing Date: 2003-11-11
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] 脳内物質の回収装置 (2003)
  • Inventor(s): 加藤啓子
  • Industrial Property Rights Holder: 奈良先端科学技術大学院大学
  • Industrial Property Number: 2003-380832
  • Filing Date: 2003-11-11
• [Publications] Matsuhashi, H., Horii, Y., Kato, K.: "Region-specific and epileptogenic-dependent expression of six subtypes of α2,3-sialyltransferase in the adult mouse brain."J Neurochem. 84. 53-66 (2004)