Utility of LPL transgenic rabbits as a human disease model
| Project/Area Number |
15500299
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Saga University (2004-2005)
佐賀医科大学 (2003) |
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Principal Investigator |
KITAJIMA Shuji Saga University, Analytical Research Center for Experimental Sciences, Research Associate, 総合分析実験センター, 助手 (70284643)
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| Co-Investigator(Kenkyū-buntansha) |
MORIMOTO Masatoshi Saga University, Analytical Research Center for Experimental Sciences, Associate Professor, 総合分析実験センター, 助教授 (90136482)
FAN Jianglin University of Yamanashi, Department of Molecular Pathology, Professor, 医学部, 教授 (60272192)
渡辺 照男 佐賀大学, 副学長 (40037396)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2004: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Obesity / Lipoprotein lipase / Insulin resistant / Transgenic / Rabbit / リボ蛋白リパーゼ |
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| Research Abstract |
In this study, we examined glucose tolerance and insulin sensitivity under chow diet or high-fat diet (HFD) loading in lipoprotein lipase (LPL) transgenic rabbits. The results are summarized below.
1.Under chow diet condition, intravesous glucose tolerance test (IVGTT), insulin tolerance test (IVTT) and hyper-euglycemic clamp test revealed that LPL transgenic rabbits have increased insulin sensitivity.
2.In obese condition induced by HFD feeding, overexpression of LPL prevented accumulation of visceral fat and improved insulin sensitivity compared with non-transgenic control rabbits. Moreover, pathological examination revealed that LPL transgenic rabbit has small adipocytes in size and fatty liver according to the deposition of triglycerides.
3.In mating LPL transgenic rabbit with WHHL rabbits which is an animal model for familial hyperlipidemia, LPL transgenic WHHL rabbits also showed amelioration of insulin sensitivity after feeding of HFD to induce insulin resistance according to obesity under hyperlipidemia.
These observations in our study suggested that overexpression of LPL in rabbits may inhibit the accumulation of fat and improve the insulin sensitivity under HFD-induced obesity. This can be an interesting results compare with that in the mice reported LPL transgenic mice showed rather than insulin resistance.
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Report
(4 results)
Research Products
(34 results)
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[Journal Article] Macrophage elastase (MMP-12) accelerates the progression of atherosclerosis in transgenic rabbits.2006
Author(s)
Liang J., Liu E., Yu Y., Kitajima S., Koike T., Jin Y., Morimoto M., Watanabe T., Hatakeyama K., Asada Y., Sasaguri Y., Watanabe S., Fan J.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] High lipoprotein lipase activity increases insulin sensitivity in transgenic rabbits.2005
Author(s)
Liu.E., Kitajima, S., Higaki, Y., Morimoto, M., Sun, H., Yamada, N., Fan, J.
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Journal Title
Metabolism. 54
Pages: 132-138
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Macrophage-derived lipoprotein lipase increases aortic atherosclerosis in cholesterol-fed transgenic rabbits.2005
Author(s)
Ichikawa T, Liang J, Kitajima S, Koike T, Wang X, Sun H., Morimoto M, Shikama H, Watanabe T, Yamada N, Fan J.
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Journal Title
Atherosclerosis 179
Pages: 87-95
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Enhanced aortic atherosclerosis in transgenic Watanabe heritable hyperlipidemic rabbits expressing lipoprotein lipase.2005
Author(s)
Koike, T., Liang, J., Wang, X., Ichikawa, T., Shiomi, M., Sun, H., Watanabe, T., Liu, G., Fan, J.
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Journal Title
Cardiovasc.Res. 65
Pages: 524-534
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Establishment of a SPF colony of human apo(a) transgenic rabbits by frozen-thawed embryo transfer..2005
Author(s)
Watanabe, N., Nakagawa, H., Kitajima, S., Liu, E., Morimoto, M, Watanabe, T, Fan, J.
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Journal Title
Exp.Anim. 54(4)
Pages: 353-357
NAID
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] C-reactive protein in atherosclerotic lesions : its origin and pathophysiological significance.2005
Author(s)
Sun, H., Koike, T., Ichikawa, T., Hatakeyama, K., Shiomi, M., Zhang, B., Kitajima, S., Morimoto, M., Watanabe, T., Asada, Y., Chen, YE., Fan, J.
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Journal Title
Am.J.Pathol. 167
Pages: 1139-1148
Description
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[Journal Article] Overexpression of lipoprotein lipase in transgenic rabbits leads to increased small dense LDL in plasma and promotes atherosclerosis.2004
Author(s)
Ichikawa T, Kitajima S, Liang J, Koike T, Wang X, Sun H, Okazaki M, Morimoto M, Shikama H., Watanabe T, Fan, J.
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Journal Title
Lab.Invest. 84
Pages: 715-726
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Overexpression of lipoprotein lipase improves insulin resistance induced by a high-fat diet in transgenic rabbits.2004
Author(s)
Kitajima S, Morimoto M, Liu E, Koike T, Higaki Y, Taura Y, Mamba K, Itamoto K, Tsutsumi K., Yamada N, Watanabe T, Fan J.
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Journal Title
Diabetologia 47
Pages: 1202-1209
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Overexpression of Lipoprotein lipase in transgenic Watanabe heritable hyperlipidemic rabbits improves hyperlipidemia and obesity.2004
Author(s)
Koike T, Liang J, Wang X, Ichikawa T, Shiomi M, Liu G, Sun H, Kitajima S, Morimoto M, Watanabe T, Yamada N, Fan J.
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Journal Title
J.Biol.Chem. 279
Pages: 7521-7529
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Overexpression of human matrix metalloproteinase-12 enhances the Development of inflammatory arthritis in transgenic rabbits.2004
Author(s)
Wong, X., Liang, J., Koike, T., Sun, H, Ichikawa, T., Kitajima, S., Morimoto, M., Shikama, H., Watanabe, T., Sasaguri, Y., Fan, J.
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Journal Title
Am.J Pathol. 165
Pages: 1375-1383
Description
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