| Project/Area Number |
15500306
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Tokyo Metropolitan Organization for Medical Research |
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Principal Investigator |
TAYA Choji TOKYO METROPOLITAN ORGANIZATION FOR MEDICAL RESEARCH, THE TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE, SENIOR RESEARCHER, 東京都臨床医学総合研究所, 主任研究員 (90175456)
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| Co-Investigator(Kenkyū-buntansha) |
KIKKAWA Yoshiaki TOKYO METROPOLITAN ORGANIZATION FOR MEDICAL RESEARCH, THE TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE, RESEARCHER, 東京都臨床医学総合研究所, 研究員 (20280787)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2005: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2004: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | SEX-DEPENDENT MEIOTIC RECOMBINATION FREQUENCY / TELOMERIC REGION / COLD SPOT / HOT SPOT / STRAIN DIFFERENCE / cold spot / hot spot系統差 / hot spot / 系統差 |
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| Research Abstract |
We found that the male' s recombination frequency was as ten times much as the female' s one on the distal part of chromosome 15 by PCR-SSCP method with microsatellite markers on two intersubspecific backcross panels, [(B6^<Ca>xMSM)x MSM]N_2 (n=595) and [B6x(B6^<Ca>xMSM)]N_2 (n=325). This shows that a sex-dependent recombination hot or cold spot exists on this chromosome region. To investigate whether this spot is hot or cold, we estimated the physical distance between microsatellite markers, D15Mit40 and D15Mit15, on this region by physical mapping with BAC clones. The resultant physical distance was about one mega-base pair. As previously reported the genetic distance was 0.17cM in female and 5. 23cM in male between these markers. A comparison of an average recombination frequency per physical distance on mouse genome with the observed genetic distances indicated that a cold spot exists in female, a hot one in male on this region. Several crosses of mouse strains suggested that this sex-dependent difference of recombination frequency depended on strains used. Genome wide research is now in progress on recombination frequencies of females and males. Preliminary results suggest that several high frequency spots of recombinations exist in the mouse genome.
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