| Project/Area Number |
15500316
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kyoto University |
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Principal Investigator |
NEO Masashi Kyoto University, Faculty of Medicine, Lecturer, 医学研究科, 講師 (80311736)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Takashi Kyoto University, Faculty of Medicine, Professor, 医学研究科, 教授 (10201675)
YAO Takashi Kyoto University, Graduate School of Energy Science, Professor, エネルギー科学研究所, 教授 (50115953)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2003: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Apatite / Coating / Micropattern / Bone formation / Osteoblast / Bioactive / Biomimetic / ハイドロキシアパタイト / アルカリフォスファターゼ |
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| Research Abstract |
As a preliminary experiment, the effect of hydroxyapatite coating on osteoblasts activity was examined. Osteoblast-like cells (MC3T3-E1) were cultured on polyethersulfone plates with or without biomimetic hydroxyapatite coating. The cells on the coated plates produced more proteins, suggesting more proliferation than the cells on the non-coated plates. On the contrary, the cells on the non-coated plates showed more alkaliphosphatase activity, suggesting more differentiation. Next, the cells were cultured on three kinds of plates with micropattern coating, that is, parallel line pattern (50 and 100 μm) and dot pattern. However, these plates did not accelerate cell proliferation nor differentiation compared with total-coated or non-coated plates in the preliminary experiments. Therefore we tried to make minuter micropattens, which may change the osteoblast activity. However, it was difficult to make a unifirm, reproducible pattern due to detachment of the coated apatite. Four different coating methods were tried without success. Recently, it was found that uniform micropattern was successfully made by precise control of concentration, pH, and temperature of the simulated body fluid solution.
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