| Project/Area Number |
15500328
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Biomedical engineering/Biological material science
|
|---|
| Research Institution | Tokyo Women's Medical University |
|---|
Principal Investigator |
GOYA Nobuyuki Tokyo Women's Medical University, Dept of Urology, Associate Professor, 医学部, 助教授 (40142492)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TOMIZAWA Yasuko Tokyo Women's Medical University, Dept of Cardiovascular Surgery, Instructor, 医学部, 助手 (00159047)
|
|---|
| Project Period (FY) |
2003 – 2004
|
| Project Status |
Completed (Fiscal Year 2004)
|
| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2004: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2003: ¥2,000,000 (Direct Cost: ¥2,000,000)
|
|---|
| Keywords | tissue engineering / angiogenesis / wound healing / scaffold / REC / remodeling / sprout formation / vessel / 遠隔期 / 配向性 / angiopolarity / アテロコラーゲン / グラフト / 代謝 / 細胞 |
|---|
| Research Abstract |
The progress in the field of tissue engineering is rapid. We expect that the latest innovations will solve all the problems associated with the current disadvantages of implantable biomaterials, such as deterioration, scar formation and calcification. Recently, atrophic changes of implanted tissue with reduction of vessel density have been noticed, especially in non-functional cosmetic implants. Due to unequal biocompatibility of biomaterials, "angiopolarity", defined as the orientation or direction of angiogenesis into biomaterials after implantation, occurs in the process of wound healing and should influence metabolic stability in the long-term. To investigate cell affinity and angiopolarity, we evaluated commercially available local hemostats, injectable collagen and other biomaterials using a rabbit ear chamber wound healing model. We observed smooth angiogenesis with a cotton-type collagen scaffold made of atelocollagen, since the movement of body fluid from respiration and venous pulse wave were not restricted by the implanted material and they propagated equally at the edge and in the center of the rabbit ear chamber. When vessel sprouts extend to and reach the collagen fiber, they crossed the fiber by going over the ridge. However, cell affinity was not satisfactory in most of the commercially available collagen hemostatic agents tested. We believed that good-angiopolarity just after implantation is the key factor to obtain stability of tissue engineered biomaterial in the long-term.
|
