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Synthesis and Evaluation of Peptide-based Hybrid Materials for Tissue Engineering

Research Project

Project/Area Number 15500333
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionOsaka Institute of Technology

Principal Investigator

HIRANO Yoshiaki  Osaka Institute of Technology, Faculty of Engineering, Associate Professor, 工学部, 助教授 (80247874)

Co-Investigator(Kenkyū-buntansha) OKA Masahito  Osaka Prefecture University, Advance Science and Technology, Associate Professor, 先端科学研究所, 助教授 (70203966)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2003: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsCell-attachment peptide / RGDS / Tissue Engineering / Scaffold / RGDS-natural polymer hybrid / Alginate / Conformational Analysis / Structure-activity relationship / Arg-Gly-Asp-Ser (RGDS) / RGDS ペプチド-高分子複合体
Research Abstract

In tissue engineering and wound-healing application, scaffold materials are utilized to provide a mechanical support for cell growth and tissue formation. These scaffold can be modified such that they also provide specific biologic signals to cell in order to control or facilitate tissue formation or regeneration. Cell adhesion peptides have been incorporated into scaffolds to enhance cell adhesion or to allow biospecific cell adhesion.
It is shown that oligopeptides having the Arg-Gly-Asp (RGD) sequence, which is related to the cell-attachment activity of fibronectin, vitronectin, collagen, and fibrinogen, may act as the ligands for the cell-attachment receptors on the cell membranes. It is also shown that the cell-attachment activity site of fibronectin and its related peptides form compactly folded conformations.
In this work, for improving the cell-attachment activity of the Arg-Gly-Asp-Ser peptides to tissue engineering scaffold, we tried to stabilize the folded conformations of suc … More h peptides by designing the ss-hairpin peptides, which have the Arg-Gly-Asp-Ser sequence at the turn portion. These sequence, Ala-Glu-Ala-Glu-Ala-Lys-Ala-Lys (EAK8), (Ala-Glu-Ala-Glu-Ala-Lys-Ala-Lys)_2 (EAK16) or (Arg-Ala-Arg-Ala-Asp-Ala-Asp-Ala)_2 (RAD 16) of the peptides is selected as a typical sequence for having high propensity to form ss-strands.
RGDS containing ss-sheet model peptides were synthesized using liquid and solid phase procedures. All peptides were characterized by NMR,MALDI-TOF MS, amino acid analysis, and elemental analysis. Cell-attachment activities of these peptides were examined by cell-attachment test using L929 fibroblast cell toward peptide-immobilized substrate.
L929 cells attaching to the peptide-immobilized cell culture dishes, indicating that difference in the cell-attachment activity were found for these peptides-immobilized cell culture dishes. These results suggest that the RGDS sequence of designed peptides commonly interact with integrin receptor of the cell. EAK16RGDS and RAD 16RGDS immobilized polystyrene-dish has the highest spreading activity among the peptide-immobilized ones.
The conformational constraint caused by the formation of ss-hairpin structure is too strong to stabilize the desired conformation at the Arg-Gly-Asp-Ser portion for the peptides in which the Arg-Gly-Asp-Ser sequence is directly linked to the sequences designed for forming ss-hairpin structure. It is also suggested that designing a spacer sequence is desirable for improving the activity between the Arg-Gly-Asp-Ser sequence and the sequence forming ss-strand structure. It was possible to prepare the tissue engineering 3D scaffold with comparative ease. Less

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (28 results)

All 2005 2004 2003 Other

All Journal Article (21 results) Book (2 results) Publications (5 results)

  • [Journal Article] On the Stability of Polyproline-I and II Structure of Proline Oligopeptides2005

    • Author(s)
      Sachiro Kakinoki, Yoshiaki Hirano et al.
    • Journal Title

      Polymer Bulletin 53

      Pages: 109-115

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] On the Stability of Polyproline- I and II Structure of Proline Oligopeptides2005

    • Author(s)
      Sachiro Kakinoki, Yoshiaki Hirano, Masahito Oka
    • Journal Title

      Polymer Bulletin 53

      Pages: 109-115

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular Design of β-Hairpin Peptides Having RGDS Sequence for Tissue Engineering2005

    • Author(s)
      Yoshiaki Hirano, Naoki Nishishita, Tomonori Ikai, Tadashi Iuchi, Mitsutaka Kayahara, Masahito Oka
    • Journal Title

      Peptide Science 2004

      Pages: 381-384

    • NAID

      10014460255

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Conformational Analysis of Polypeptides Having Repetitive Ser-Pro Sequences2005

    • Author(s)
      Yoshiaki Hirano, Tomoyuki Yoshikawa, Masaki Okada, Sachiro Kakinoki, Masahito Oka
    • Journal Title

      Peptide Science 2004

      Pages: 445-448

    • NAID

      10014460381

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Conformational Analysis of Polypeptides Having Repetitive Xaa-Pro Sequences2004

    • Author(s)
      Yoshiaki Hirano, et al.
    • Journal Title

      Peptide Science 2003

      Pages: 399-402

    • NAID

      10012654482

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Conformational Analysis of Polypeptides Having Repetitive Xaa-Xaa-Pro Sequences2004

    • Author(s)
      Yuichi Onoda, Yashiaki Hirano, et al.
    • Journal Title

      Peptide Science 2003

      Pages: 403-406

    • NAID

      10012654490

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Conformational Analysis of Polypeptides Having Repetitive Xaa-Xaa-Xaa-Pro Sequences2004

    • Author(s)
      Mitsuo Arimoto, Yoshiaki Hirano, et al.
    • Journal Title

      Peptide Science 2003

      Pages: 407-410

    • NAID

      10012654492

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] β-シート構造を有する細胞接着性ペプチドの設計2004

    • Author(s)
      井内 匡, 平野義明 他
    • Journal Title

      バイオマテリアル-生体材料- 22

      Pages: 281-292

    • NAID

      10013078481

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Effect of Culture Substrates and Fibroblast Growth Factor Addition on the Proloferation and Differentiation of Rat Bone Marrow Stromal Cells2004

    • Author(s)
      Akihiko Kanematsu, Yoshiaki Hirano, et al.
    • Journal Title

      TISSUE ENGINEERING 10

      Pages: 995-1005

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Peptide and Protein Presenting Materials for Tissue Engineering2004

    • Author(s)
      Yoshiaki HIRANO, David J.Mooney
    • Journal Title

      Advanced Materials 16, 1

      Pages: 17-25

    • NAID

      10024920411

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Conformational Analysis of Polypeptides Having Repetitive Xaa-Pro Sequences2004

    • Author(s)
      Yoshiaki Hirano, Masaki Okada, Tsuyoshi Tozawa, Tadashi Iuchi, Masahito Oka
    • Journal Title

      Peptide Science 2003

      Pages: 399-402

    • NAID

      10012654482

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Conformational Analysis of Polypeptides Having Repetitive Xaa-Xaa-Pro Sequences2004

    • Author(s)
      Yuichi Onoda, Sachiro Kakinoki, Masahito Oka, Yoshiaki Hirano
    • Journal Title

      Peptide Science 2003

      Pages: 403-406

    • NAID

      10012654490

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Conformational Analysis of Polypeptides Having Repetitive Xaa-Xaa-Xaa-Pro Sequences2004

    • Author(s)
      Mituo Arimoto, Sachiro Kakinoki, Masahito Oka, Yoshiaki Hirano
    • Journal Title

      Peptide Science 2003

      Pages: 407-410

    • NAID

      10012654492

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Type I collagen can function as a reservoir of basic fibroblast growth factor2004

    • Author(s)
      Akihiro Kanematsu, Akira Marui, Shingo Yamamoto, Makoto Ozeki, Yoshiaki Hirano, Masaya Yamamoto, Osamu Ogawa, Masashi Komeda, Yasuhiko Tabata
    • Journal Title

      Journal of Controlled Release 99, 2004

      Pages: 281-292

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effect of Culture Substrates and Fibroblast Growth Factor Addition on the Proloferation and Differentiation of Rat Bone Marrow Stromal Cells2004

    • Author(s)
      YUKANA HORI, SACHIKO INOUE, YOSHIAKI HIRANO, YASUHIKO TABATA
    • Journal Title

      TISSUE ENGINEERING 10, 7/8

      Pages: 995-1005

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Conformational Analysis of Polypeptides Having Repetitive Xaa-Xaa-Pro Sequences2004

    • Author(s)
      Yuichi Onoda, Yoshiaki Hirano, et al.
    • Journal Title

      Peptide Science 2003

      Pages: 403-406

    • NAID

      10012654490

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Molecular Design of b-Hairpin Peptides Having Arg-Gly-Asp-Ser Sequence2003

    • Author(s)
      Tadashi Iuchi, Mitsutaka Kayahara, Yukana Hori, Masahito Oka, Toshio Hayashi, Yoshiaki Hirano
    • Journal Title

      Peptide Science 2002

      Pages: 361-364

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Conformational Analysis of Model Polypeptides Having Repetitive Xaa-Pro-Pro-Pro Sequences2003

    • Author(s)
      Tatsuya Nakamura, Yuichi Onoda, Sachiro Kakinoki, Masahito Oka, Toshio Hayashi, Masahiro Hattori, Yoshiaki Hirano
    • Journal Title

      Peptide Science 2002

      Pages: 341-344

    • NAID

      80015974601

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Conformational Analysis of Model Polypeptides Having Repetitive Ala-Ala-Pro Sequences2003

    • Author(s)
      Hitoshi Akiyama, Sachiro Kakinoki, Masahito Oka, Toshio Hayashi, Masahiro Hattori, Mitsuo Arimoto, Yoshiaki Hirano
    • Journal Title

      Peptide Science 2002

      Pages: 345-348

    • NAID

      80015974602

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Regulating Bone Formation via Controlled Scaffold Degradation2003

    • Author(s)
      Eben Alsbeng, Hyon-Joon Kong, Yoshiaki Hirano, Molly K.Smith, A.Albeiruti, David J.Mooney
    • Journal Title

      J.Dental Research 82, 11

      Pages: 903-908

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Phosphorylcholine-endcapped oligomer and co-oligomer and surface biological reactivity2003

    • Author(s)
      Takehisa Matsuda, Junichi Nagase, Akane Ghoda, Yoshiaki Hirano, Satoru Kidoaki, Yasuhide Nakayama
    • Journal Title

      Biomaterials 24, 24

      Pages: 4517-4527

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 再生医療へのブレイクスルー その革新技術と今後の方向性2004

    • Author(s)
      平野義明 他
    • Publisher
      株式会社 メディカル ドゥ
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 再生医療へのブレイクスルー その革新技術と今後の方向性2004

    • Author(s)
      平野義明 他
    • Total Pages
      308
    • Publisher
      株式会社メディカルドゥ
    • Related Report
      2004 Annual Research Report
  • [Publications] T.Nakamura, Y.Hirano et al.: "Conformation Analysis of Model Polypeptides Having Repetitive Xaa-Pro-Pro-Pro Sequences"Peptide Science. 2002. 341-344 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Iuchi, Y.Hirano et al.: "Molecular Design of β-Hairpin Peptides Having Arg-Gly-Asp-Ser Sequence"Peptide Science. 2002. 361-364 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 平野義明, 井内 匡: "細胞接着性ペプチドとTissue Engineering"接着. 47・12. 543-550 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Y.Hirano, D.J.Mooney: "Peptide and Protein Presenting Materials for Tissue Engineering"ADVANCED MATERIALS. 16・1. 17-25 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] E.Alsberg, H.Hirano et al.: "Regulating Bone Formation Via Controlled Scaffold Degradation"Research Reports Biomaterials & Bioengineering. 82・11. 903-908 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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