Diagnosis of tumor neovascularization using matri-gel and contrast

• Project/Area Number: 15500347
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Medical systems
• Research Institution: Tokyo Medical University
• Principal Investigator: MORIYASU Fuminori Tokyo Medical University., Medicine, Professor, 医学部, 教授 (80191055)
• Co-Investigator(Kenkyū-buntansha): IIJIMA Hiroko Tokyo Medical University, Medicine, Associate Professor, 医学部, 講師 (80289066) ; KAMIYAMA Naohisa Toshiba Medical System Co., Department of Research and Development, Chief engineer, 医用システム社・超音波開発部, 研究員
• Project Period (FY): 2003 – 2004
• Project Status: Completed (Fiscal Year 2004)
• Budget Amount: ¥3,600,000 (Direct Cost: ¥3,600,000); Fiscal Year 2004: ¥900,000 (Direct Cost: ¥900,000); Fiscal Year 2003: ¥2,700,000 (Direct Cost: ¥2,700,000)
• Keywords: contrast ultrasound / microbubble / flash replenishment imaging / harmonic imaging / tumor neovascularity / parametric imaging / Micro Flow Imaging / arrival time imaging / 超音波画像 / 超音波診断装置 / 血管腫

Research Abstract

We have developed the new method to visualize neovascularized tumor blood vessels of the liver. It is a method using ultrasound contrast imaging. It uses a real time and low mechanical index (MI) ultrasound imaging and it can visualize microbubbles when they reaches at the liver parenchyma after intravenous injection. The method is the phase inversion harmonic imaging and also composed of replenishment imaging. Furthermore, we have introduced a new method, Micro Flow Imaging, which utilized a maximum intensity holding technique.
We have applied these techniques to investigate tumor neovasculization of the animal models. By using these technique, micro blood vessels which size is less than 100 micro meters were visualized with a good continuity. At the same time, blood flow parameters of the tumor blood vessels such as arrival time and peak time were analyzed in the tumor vessels. These parameters were imaged as parametric imaging and they could indicate characteristics of the malignant liver tumors.
Both of morphological and functional features of the malignant liver tumors were evaluated using the newly developed imaging methods and it is concluded that these technique could be applied to clinical diagnosis of liver tumors.

Research Products

• [Journal Article] II腹部 1.腹部領域における超音波技術の新しい潮流 1)コントラストエコー -次世代造影剤とその臨床応用- (2005)
  • Author(s): 森安史典, Willson, S.R.
  • Journal Title: 総合的臨牀54 増刊 臨床医のための超音波診断アトラス 54 Pages: 300-304
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Immunohistochemical analysis of p53 and MIB-1 in tissue specimens obtained from endoscopic ultrasonography-guided fine needle aspiration biopsy for the diagnosis of solid pancreatic masses. (2005)
  • Author(s): Itoi T, Takei K, Sofuni A, Itokawa F, Tsuchiya T, Kurihara T, Nakamura K, Moriyasu F, Tsuchida A, Kasuya K.
  • Journal Title: Oncol Rep. 13(2) Pages: 229-234
• [Journal Article] Differential diagnosis of hepatic nodules using delayed parenchymal phase imaging of levovist contrast ultrasound : comparative study with SPIO-MRI. (2004)
  • Author(s): Suzuki, S., Iijima, H., Moriyasu, F., Sasaki, S, Yanagisawa, K., Miyahara, T., Oguma, K., Yoshida, M., Horibe, T., Ito, N., Kakuzaki, D., Abe, K., Tsuchiya, K.
  • Journal Title: Hepatol Res 29(2) Pages: 122-126
  • NAID: 10018024399
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Usefulness of contrast-enhanced ultrasonography with a new contrast mode, agent detection imaging in evaluating therapeutic response in hepatocellular carcinoma treated with radio-frequency ablation therapy (2004)
  • Author(s): Shimizu, M., Iijima, H., Horibe, T., Yamada, M., Suzuki, S., Yanagisawa, K., Seki, T., Moriyasu, F.
  • Journal Title: Hepatol Res. 29(4) Pages: 235-242
  • NAID: 10018024952
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Differential diagnosis of hepatic nodules using delayed parenchymal phase imaging of levovist contrast ultrasound: comparative study with SPIO-MRI. (2004)
  • Author(s): Suzuki, S., Iijima, H., Moriyasu, F., Sasaki, S., Yanagisawa, K., Miyahara, T., Oguma, K., Yoshida, M., Horibe, T., Ito, N., Kakizaki, D., Abe, K., Tsuchiya, K.
  • Journal Title: Hepatol Res. 29(2) Pages: 122-126
  • NAID: 10018024399
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Usefulness of contrast-enhanced ultrasonography with a new contrast mode, agent detection Imaging, in evaluating therapeutic response in hepatocellular carcinoma treated with radio-frequency ablation therapy (2004)
  • Author(s): Shimizu, M., Iijima, H., Horibe, T., Yamada, M., Suzuki, S., Yanagisawa, K., Seki, T., Moriyasu, F.
  • Journal Title: Hepatol Res. 29(4) Pages: 235-242
  • NAID: 10018024952
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Microvascular imaging of the liver using a new contrast mode, Micro Flow Imaging and next generation contrast agents(Keynote Lecture) (2004)
  • Author(s): Moriyasu, F., Iijima, H., Kamiyama, N., Sugimoto, K., Metoki, R., Yosida, M., Oguma, K., Suzuki, S., Shimizu, M.
  • Journal Title: 7^<th> Congress of the Asian Federation of Societies for Ultrasound in Medicine and biology (AFSUMB 2004)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Micro flow imaging and arrival time parametric imaging of the liver (2004)
  • Author(s): Kamiyama, N., Iijima, H., Moriyasu, F.
  • Journal Title: 9^<th> European Symposium on Ultrasound Contrast Imaging
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Differetiated diagnosis of histology of pancreatic carcinoma using ultrasound contrast imaging (2004)
  • Author(s): Sofuni, A., Itoi, T., Nakayama, D., Itokawa, F., Nakamura, K., Iijima, H., Moriyasu, F.
  • Journal Title: AGA2004 (The American Gastroenterological Association )
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Phase modulation and amplitude modulation methods in ultrasound contrast imaging of the liver with next generation contrast agents (2004)
  • Author(s): Moriyasu, F., Sato, T., Kamiyama, N.*, Mine, Y.*, Metoki, R., Sugimoto, K., Sasaki, S., Iijima, H.
  • Journal Title: 7th Congress of the Asian Federation of Societies for Ultrasound in Medicine and biology (AFSUMB 2004)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Microvascular imaging of the liver tumor using a sequence of ultrasound contrast replenishment (2004)
  • Author(s): Moriyasu, F., Kamiyama, N.*, Iijima, H., Metoki, R., Sugimoto, K., Sasaki, S., Suzuki, S., Yosida, M., Oguma, K., Ogasawara, Y.*
  • Journal Title: 2004 ALUM Annual Convention
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Differential diagnosis of hepatic nodules using delayed parenchymal phase imaging of levovist contrast ultrasound : comparative study with SPIO-MRI. (2004)
  • Author(s): Suzuki, S., Iijima, H., Moriyasu, F., Sasaki, S., Yanagisawa, K., Miyahara, T., Oguma, K., Yoshida, M., Horibe, T., Ito, N., Kakizaki, D., Abe, K., Tsuchiya, K.
  • Journal Title: Hepatol Res. 29(2) Pages: 122-126
  • NAID: 10018024399
• [Journal Article] 造影超音波診断の新しい展開肝疾患における造影超音波診断 次世代造影剤による造影長音波診断 (2003)
  • Author(s): 飯島尋子, 森安史典
  • Journal Title: 肝・胆・膵 47(2) Pages: 203-212
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Contrast Agent ; Phagocytosis of microbubbles by kupffer cells and delayed parenchymal imaging of the liver (2003)
  • Author(s): Moriyasu, F.
  • Journal Title: 10^<th> Congress of the World Federation for Ultrasound in Medicine and Biology
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Flash-replenishment imaging-visualization and quantification (2003)
  • Author(s): Kamiyama, N., Moriyasu, F.
  • Journal Title: 5^<th> International Symposium on Ultrasound Cotrast Imaging
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Delayed parenchymal imaging of the liver five minutes after injection of contrast agent, Levovist in patients with chronic hepatitis and chirrhosis. (2003)
  • Author(s): Sasaki, S., Iijima, H., Wanibe, M., Yoshida, M., Yamada, M., Miwa, K., Suzuki, S., Oguma, K., Horibe, T., Tsuchiya, K., Moriyasu, F.
  • Journal Title: 10^<th> Congress of the World Federation for Ultrasound in Medicine and Biology
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Evaluation of hemodynamic changes and usefulness of contrast-enhanced ultrasound with agent dection imaging for pancreatic diseases (2003)
  • Author(s): Sofuni, A., Nakayama, D., Shimizu, M., Itokawa, F, Nakamura, K., Itoi, T., Iijima, H., Moriyasu, F.
  • Journal Title: Digestive Disease Week 2003
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Is it possible to diagnose the grade of histological differentiation of pancreatic carcinoma using ultrasound contrast imaging? (2003)
  • Author(s): Sofuni, A., Itoi, T., Nakayama, D., Itokawa, F., Nakamura, K., Iijima, H., Moriyasu, F.
  • Journal Title: 11^<th> United European Gastroenterology Week
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Ultrasound contrast imasing of hepatic microcirculation using a replenishment sequence (2003)
  • Author(s): Moriyasu, F., Kamiyama, N., Iijima, H., Sasaki, S., Suzuki, S., Ogasawara, Y.
  • Journal Title: RSNA 2003-89th Scientific Assembly and Annual Meeting
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] 造影超音波診断の新しい展開 肝疾患における造影超音波診断 次世代造影剤による造影超音波診断 (2003)
  • Journal Title: 肝・胆・膵 47(2) Pages: 203-212
• [Book] 超音波造影ガイドブック〔わかりやすい基礎知識と臨床応用〕消化器 1 肝臓 A 腫瘍性病変 (2003)
  • Author(s): 飯島尋子, 森安史典
  • Publisher: 金原出版株式会社
  • Description: 「研究成果報告書概要(和文)」より