| Project/Area Number |
15500363
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Rehabilitation science/Welfare engineering
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| Research Institution | Hiroshima University |
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Principal Investigator |
YUGE Rui Hiroshima University, Graduation School of Health Sciences, Professor, 大学院・保健学研究科, 教授 (20263676)
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| Co-Investigator(Kenkyū-buntansha) |
KANNO Masamoto Hiroshima University, Graduation School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (40161393)
KATAOKA Katsuko Hiroshima University, Graduation School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (30034002)
URABE Yukio Hiroshima University, Graduation School of Health Sciences, Professor, 大学院・保健学研究科, 教授 (40160337)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2004: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | mesenchymal stem cells / chondrogenesis / microgravity / cell growth / cell differentiation / MAPK-cascade / high-density culture / telomere / 細胞骨格 |
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| Research Abstract |
A three-dimensional (3D) clinostat is a device for multi-direction G force generation, which produces an environment with an average of 10-3 G over time. Behavior of human somatic cells under microgravity environment is only poorly understood and is unexpectable showing ignorance of our knowledge. We report here that human mesenchymal stem cells (hMSCs) in the 3D-clinostat (group CL) proliferated more extensively than those in normal 1G environment (group C). FACS showed that the number of hMSCs double-positive for CD 44/CD 29 or CD 90/CD 29 were 6-times higher in group CL as compared with group C after 7 days culture. Telomere length of hMSCs did not change during culture periods in both groups, CL and C. Telomerase activity was undetectable level in all these hMSC cells. The expression levels of type II collagen, aggrecan increased in the group C but decreased and could not be detected in the group CL. Phosphorylation of ERK1/2 was increased and p38MAPK was repressed in the group CL being consistent with stimulated proliferation and inhibited differentiation. When the pellets of hMSCs were explanted into the cartilage defect made in the intercondylar fossa of the mouse femur, the transplants from group CL formed hyaline cartilage after 7 days, whereas the transplants from group C formed non-cartilage tissue containing a small number of cells. These results show that hMSCs cultured in a 3D-clinostat extensively proliferate with characteristics of stem cells keeping their ability to differentiate into hyaline cartilage after transplantation, which cannot be performed by culturing in 1G condition. Simulated microgravity may be useful for the expansion of stem cells in vitro and could be applicable for regeneration medicine and developmental biology.
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