| Co-Investigator(Kenkyū-buntansha) |
ONOZAWA Haruko Showa Women's University, Graduate School of Human Life Sciences, Professor, 生活機構研究科, 教授 (10054149)
KOMIYAMA Jiro Jissen Women's University, Faculty of Human Science, Professor, 生活科学部, 教授 (60016574)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2004: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2003: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Research Abstract |
We have been studying the detergency processes of various soils mainly by using quartz microbalance method. The method uses the 9 MHz quartz crystal oscillator in water to measure a minute weight changes of μg order model soils attached on the gold electrode of the oscillator. Absorption of a trace amount of a substance in the soil decreases the frequency by 1Hz/ing. Removal of the soil increases the frequency to the same extent. Thus, we can measure the detergency process as swelling and the following removal of the soil with time. We had studied the removal of model protein soils by surfactants, oxidizing agents and proteases before studying the removal of lipid soils. With model lipids, we have reported the mechanism of the removal by adding alkali and surfactants.
This study extends the lipid detergency study to the effects of adding lipase and oxidizing agent. The processes of removal of model lipid soils, tripalmitin, trimiristin and lard in the presence of a lipase, Lipex and ani
… More
onic and non-ionic surfactants, DBS and APE, respectively, were studied in a solution of pH 7 at 30゜C. Synergies of the lipase and the surfactants were observed. The removal was enhanced in the presence of the lipase and DBS at the concentrations far below the cmc, but it was suppressed above the cmc of DBS. On the other hand, the removal was enhanced in the presence of the lipase and APE at the concentrations above the cmc. The reasons for these synergies were discussed. In terms of the negative charges endowed to the lipid layer and the lipase by DBS micell, both become repulsirve. In terms of the hydrophobicity endowed to the lipid layer and the lipase by APE micell, both become attractive.
As for oxidizing agent, the addition resulted in the enhanced swelling of the model soils. Afterward, the lipid soils become faster to be removed. This means that oxidizing agent attacks the lipid effectively to oxidize, resulting in the enhanced swelling of the lipid layer by increasing the hydrophilicity. Less
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