Evaluation of Combined Toxic Potency of Xenobiotics and the Decreasing Effect of Edible Plants

• Project/Area Number: 15500545
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Eating habits, studies on eating habits
• Research Institution: University of Shizuoka
• Principal Investigator: KINAE Naohide Univ. of Shizuoka, Sch. of Food & Nutr. Sci., Prof., 食品栄養科学部, 教授 (00046286)
• Co-Investigator(Kenkyū-buntansha): MASUDA Shuichi Univ. of Shizuoka, Sch. of Food & Nutr. Sci., Assist. Prof., 食品栄養科学部, 助手 (40336657)
• Project Period (FY): 2003 – 2004
• Project Status: Completed (Fiscal Year 2004)
• Budget Amount: ¥2,100,000 (Direct Cost: ¥2,100,000); Fiscal Year 2004: ¥800,000 (Direct Cost: ¥800,000); Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
• Keywords: mutagenicity / combined effect / nitroso compounds / antimutagenicity / tea catechin / comet assay / micronucleus test / goldfish / 変異原 / 発がん物質 / 複合作用 / コメットアッセイ

Research Abstract

A mixture of bisphenol A (BPA) and sodium nitrite was dissolved in acetate buffer (pH3.0) and incubated at 37℃ for 60 min, a new mutagens having hydroxyl and azo groups was isolated. When a mixture of morpholine and sodium nitrite was used, nitrosomorpholine (NMo) was produced. But the addition of tea extract decreased the mutagnnicity and the content of NMo. These data demonstrated the combined effects between environmental chemicals and sodium nitrite that is present in vegetables and saliva were observed.
By comparison of the genotoxicity of micronucleus test and comet assay using rodent or fish, goldfish was found to be a useful candidate as the experimental animal.

Research Products

• [Journal Article] Inhibitory effects of Gochoonangi(Wasabia japonica) against Helicobacter pylori and its urease activity (2004)
  • Author(s): Hideki Masuda, Naohide Kinae et al.
  • Journal Title: Food Science Biotechnology 13・2 Pages: 191-196
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Bactericidal activity of wasabi (Wasabia japonica) against Helicobacter pylori (2004)
  • Author(s): II Shik Shin, Naohide Kinae et al.
  • Journal Title: Journal of Food Microbaiology 94 Pages: 255-261
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Depressive symptoms are independently correlated with lipid peroxidation in a female population Comparison with vitamins and carotenoids (2004)
  • Author(s): Hirohito Tsuboi, Naohide Kinae et al.
  • Journal Title: Journal of psychosomatic resarch 56 Pages: 53-58
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Genotoxicity of 2-[2-(acetylamino)-4-[bis(2-hydroxyethyl)amino]-5-methoxyphenyl]-5-amino-7-bromo-4-chloro-2H-benzotriazole (PBTA-6) and 4-amino-3,3'-dichloro-5,4'-dinitro-biphenyl (ADDB) in goldfish (Carassius auratus) using the micronucleus test and the comet assay. (2004)
  • Author(s): Shuichi Masuda, Naohide Kinae et al.
  • Journal Title: Mutation Research 560 Pages: 33-40
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] 緑茶抽出物のニトロソ化抑制効果と疫学調査 (2004)
  • Author(s): 増田修一, 木苗直秀
  • Journal Title: 環境変異原研究 26・3 Pages: 265-273
  • NAID: 110001713963
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] 水道水中の強力な変異原物質3-chloro-4(dichloromethyl)-5-hydroxy-2(5H)-furanone(MX)の分布と毒性 (2004)
  • Author(s): 杉山千歳, 木苗直秀
  • Journal Title: 水環境学会誌 27・6 Pages: 393-401
  • NAID: 10013080006
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Bactericidal activity of wasabi(Wasabia japonica) against Helicobacter pylori (2004)
  • Author(s): Shin, IL Shik, Masuda, Hideki, Kinae, Naohide
  • Journal Title: International Journal of Food Microbiology 94 Pages: 255-261
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Inhibitory Effects of Gochoonangi(Wasabia japonica) against Helicobacter pylori andits unease activity (2004)
  • Author(s): Masuda, Hideki, Kinae, Naohide, Woo, Gun-Jo, Shin, Il-Shik
  • Journal Title: Food Science and Biotechnology 13(2) Pages: 191-196
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Inhibitory Effects of green tea extracts on nitrosation and epidemiological study on cancer mortality (2004)
  • Author(s): Masuda, Shuichi, Uchida, Shoko, Terashima, Yumeko, Kinae, Naohide
  • Journal Title: Environ. Mutagen Res. 26 Pages: 265-273
  • NAID: 110001713963
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Depressive symptoms are independently correlated with lipid peroxidation in a female population Comparison with vitamins and carotenoids (2004)
  • Author(s): Tsuboi, Hirohito, Shimoi, Kayoko, Kinae, Naohide, Oguni, Itaro, Hori, Reiko, Kobayashi, Fumio
  • Journal Title: Journal of psychosomatic resarch 56 Pages: 53-58
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Distribution of 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone(MX) in water Samples, and its chemical and genotoxic properties (2004)
  • Author(s): Sugiyama, Chitose, Nakajima, Madoka, Iwamoto, Ken-ichi, Masuda, Shuichi, Oishi, Etsuo, Kinae, Naohide
  • Journal Title: Journal of Japan Society on Water Environment Vol.27, No.6 Pages: 393-401
  • NAID: 10013080006
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Genotoxicity of 2-[2-(acetylamino)-4-[bis(2-hydroxyethyl)amino]-5-methoxyphenyl]-5-amino-7-bromo-4-chloro-2H-benzotriazole (PBTA-6) and 4-amino-3,3'-dichloro-5,4'-dinitro-biphenyl (ADDB) in goldfish (Carassius auratus) using the micronucleus test and the comet assay (2004)
  • Author(s): Masuda, Shuichi, Deguchi, Yuya, Masuda, Yumi, Watanabe, Tetsushi, Nukaya, Haruo, Terao, Yoshiyasu, Takamura, Takeji, Wakabayashi, Keiji, Kinae, Naohide
  • Journal Title: Mutation Research 560 Pages: 33-40
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Inhibitory effects of Gochoonangi (Wasabia japonica) against Helicobacter pylori and its urease activity (2004)
  • Author(s): Hideki Masuda, Naohide Kinae et al.
  • Journal Title: Food Science Biotechnology 13(2) Pages: 191-196
• [Journal Article] Bactericidal activity of wasabi (Wasabia japonica) against Helicobacter pylori (2004)
  • Author(s): H Shik Shin, Naohide Kinae et al.
  • Journal Title: Journal of Food Microbaiology 94 Pages: 255-261
• [Journal Article] Genotoxicity of 2-[2-(acetylamino)-4-[bis(2-hydroxyethyl)amino]-5-methoxyphenyl]-5-amino-7-bromo-4-chloro-2H-benzotriazole (PBTA-6) and 4-amino-3,3'-dichloro-5,4'-dinitro-biphenyl (ADDB) in gold fish (Carassius auratus) using the micronucleus test and the comet assay. (2004)
  • Author(s): Shuichi Masuda, Naohide Kinae et al.
  • Journal Title: Mutation Research 560 Pages: 33-40
• [Journal Article] 緑茶抽出物のニトロソ化抑制効果と疫学調査 (2004)
  • Author(s): 増田修一, 木苗直秀
  • Journal Title: 環境変異原研究 26 Pages: 265-273
  • NAID: 110001713963
• [Journal Article] 生活環境中の変異原物質の分離同定とそれらの腫瘍発生との関連に関する研究 (2003)
  • Author(s): 木苗直秀
  • Journal Title: 環境変異原研究 25・2 Pages: 93-100
  • NAID: 110001708184
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Isolation and identification of environmental mutagens and their relationship with tumor induction (2003)
  • Author(s): Kinae, Naohide
  • Journal Title: Environ. Mutagen Res. 25 Pages: 93-100
  • NAID: 110001710502
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Formation of a new mutagens, 2-(5-hydroxy-4,6-dinitoroindolyl) ethanol, from the reaction of 5-hydroxytryptamine, nitrite and L-cysteine.
  • Author(s): Shuichi Masuda, Naohide Kinae et al.
  • Journal Title: Mutation Research (印刷中)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Changes in the Mutagenic and Estrogenic Activities of Bisphenol A Upon Treatment with Nitrite
  • Author(s): Shuichi Masuda, Naohide Kinae et al.
  • Journal Title: Mutation Research (印刷中)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Formation of a new mutagens, 2-(5-hydroxy-4, 6-dinitoroindolyl) ethanol, from the reaction of 5-hydroxytryptamine, nitrite and L-cysteine
  • Author(s): Masuda, Shuichi, Tan, Hiromi, Terashima, Yumeko, Hashizume, Takashi, Kumazawa, Shigenori, Sugiyama, Yasumasa, Sugimoto, Osamu, Tanji, Ken-ichi, Nakayama, Tsutomu, Terao, Yoshiyasu, Wakabayashi, Keiji, Kinae, Naohide
  • Journal Title: Mutation Research (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Changes in the mutagenic and estrogenic activity of bisphenol A upon treatment with nitrite
  • Author(s): Masuda, Shuichi, Terashima, Yumeko, Sano, Ayako, Kuruto, Ryoko, Sugiyama, Yasumasa, Shimoi, Kayoko, Tanji, Ken-ichi, Yoshioka, Hisashi, Terao, Yoshiyasu, Kinae, Naohide
  • Journal Title: Mutation Research (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Changes in the Mutagenic and Estrogenic Activities of Bisphenol A Upon Treatment with Nitrite
  • Author(s): Shuichi Masuda, Naohide Kinae et al.
  • Journal Title: Mutation Research (印刷中)
• [Publications] S.Masuda, N.Kinae et al.: "Genotoxicity of PBTA-6 and ADDB in goldfish(Carassius auratus)using the micronucleus test and the comet assay"Mutation Research. (in press).
• [Publications] 杉山千歳, 木苗直秀: "水道水中の強力な変異原物質(MX)の分布と毒性"日本水環境学会誌. (印刷中). (2004)
• [Publications] M.Watanabe, N.Kinae et al.: "Identification of 6-methylsulfinylhexyl isothiocyanate as an apoptosis-inducing component in wasabi."Phytochemistry. 65(2). 733-739 (2003)