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Study of Antibacterial Substances Found in Natto

Research Project

Project/Area Number 15500558
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Eating habits, studies on eating habits
Research InstitutionKurashiki University of Science and The Arts

Principal Investigator

SUMI Hiroyuki  Kurashiki University of Science and The Arts, Department of Life Science, Professor, 生命科学部, 教授 (00107814)

Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2004: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2003: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsBucillus subtilis natto / 2,6-pyridnedicarboxylic acid / fibrin plate / plasminogen / genus Bacillus / 抗菌活性 / プロバイオティック / リゾチーム / ニコチン酸関連物質
Research Abstract

An analysis of dipicolinic acid (DPA,2,6-pyridinedicarboxylic acid) contained in Bacillus subtilis natto cells using the colorimetric method of Janssen and others showed that the largest DPA content is approximately 2.4-3.6% of the total dry weight.
A comparison of the antibacterial activity of DPA and its derivatives-quinolic acid (2,3-pyridine dicarboxylic acid), 2,4-pyridine dicarboxylic acid, isoinchlorendic acid (2,5-pyridine dicarboxylic acid), and 3,5-pyridine dicarboxylic acid-revealed that this antibacterial activity is unique to DPA. Moreover, an investigation into the effects of various metal ions on the antibacterial activity showed that the activity is strongly inhibited through the addition of Ca^<++>,Fe^<++>,Co^<++>, and Zn^<++>, all divalent ions.
It was also shown that the addition of DPA increases the amount of nattokinase produced by Bacillus subtilis natto. In four types of Bacillus subtilis natto, the Miyagino, Meguro, Takahashi and Naruse types, DPA with a 2 mM conc … More entration increased the amount of nattokinase produced. In the case of the Miyagino type, for example, the fibrinolytic activity for fibrin plates was double the level seen for the sample with no DPA added, at 256.1 mm^2/30 μl at 37℃ after four hours (1,500 FU/ml for the nattokinase activity). A comparison test, with 10 types of nicotinic acid-related substances added, showed that dipicolinic acid had the strongest effect.
DPA with a final concentration of 5×10^<-3> mol/l was found to substantially inhibit the platelet aggregation reaction caused by human adenosine triphosphate(ATP), as well as the reaction of thrombin converting fibrinogen into fibrin. It became clear that at 5×10^<-3> mol/l, there was a complete inhibition of the blood coagulation reaction as observed in thromboelastography, while such strong effects were not observed in the case of DPA derivatives, even at 10 mM. There were no changes in human prothrombin time, the thromboplastin time for the active areas, or euglobulin lysis time.
Although platelet aggregation in human blood is observed with 30 μM of adenosine diphosphate(ADP), it became clear that dose-dependent inhibition of this aggregation occurs when there is 5×10^<-3> M of DPA. The calculated CI_<50> was at the fairly low concentration of 1.8×10^<-3> M, and far stronger (4.0×10^<-2> M) than in the case of adding aspirin for clinical use. Less

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (59 results)

All 2006 2005 2004 2003 Other

All Journal Article (42 results) Book (11 results) Publications (6 results)

  • [Journal Article] 納豆菌体のジピコリン酸含量,および強力な抗ピロリ菌活性2006

    • Author(s)
      須見洋行, 矢田貝智恵子, 丸山真杉, 池田志織, 大杉忠則
    • Journal Title

      薬理と臨床 16・3

      Pages: 261-266

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Activation of fibrinolysis (nattokinase) induced by dipicolini_cacid and related compounds2006

    • Author(s)
      Ikeda S., Ohsugi T., Sumi H.
    • Journal Title

      Food Science and Technology Research (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 納豆菌のジピコリン酸量,および強力な抗ピロリ菌活性2006

    • Author(s)
      須見洋行, 大杉忠則, 池田志織, 川原知子, 内藤佐和, 矢田貝智恵子
    • Journal Title

      日本家政学会第58回大会要旨集

      Pages: 93-93

    • NAID

      130006955195

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Dipicolinic acid in Bacillus subtilis natto and strong Anti-H. pylori Activity2006

    • Author(s)
      Hiroyuki Sumi
    • Journal Title

      Clinical Pharmacology and Therapy 16・3

      Pages: 261-266

    • NAID

      130006955195

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Dipicolinic acid in Bacillus subtilis natto and strong anti-H. pylori activity2006

    • Author(s)
      Hiroyuki Sumi
    • Journal Title

      Abstracts of the 58^<th> congress on Journal of Society Home Economics

      Pages: 93-93

    • NAID

      130006955195

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Activation of fibrinolysis (nattokinase) induced by dipicolinic acid and related compounds2006

    • Author(s)
      S.Ikeda, T.Ohsugi, H.Sumi
    • Journal Title

      Food Science and Technology Research (In press)

    • NAID

      10020354518

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 納豆菌体のジピコリン酸含量,および強力な抗ピロリ菌活性2006

    • Author(s)
      須見洋行, 矢田貝智恵子, 丸山真杉, 池田志織, 大杉忠則
    • Journal Title

      Clinical Pharmacology and Therapy 16巻3号(In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 血詮症予防に働く食品,及び機能成分2005

    • Author(s)
      須見洋行
    • Journal Title

      Priming Bin Medicine 1

      Pages: 16-24

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Natto bacillus contains a large amount of water-soluble vitamin K (menaquinone-7)2005

    • Author(s)
      Yanagisawa Y., Sumi H.
    • Journal Title

      Journal of Food Biochemistry 29・3

      Pages: 267-277

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 無蒸煮麦類の納豆菌処理による栄養価及び生理活性の付加2005

    • Author(s)
      須見洋行, 浅野倫和, 矢田貝智恵子
    • Journal Title

      日本醸造学会誌 100・6

      Pages: 449-453

    • NAID

      80017344526

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Anti-platelet aggregation and anti-blood coagulation activities of Bacillus subtilis natto component, dipicolinic acid2005

    • Author(s)
      Obsugi T., Sumi H.
    • Journal Title

      Food Science and Technology Research 11・3

      Pages: 308-310

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Functional foods and relating components for the prevention of thrombosis2005

    • Author(s)
      Hiroyuki Sumi
    • Journal Title

      Priming Bio Medicine 1

      Pages: 16-24

    • NAID

      40015482260

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Natto bacillus contains a large amount of water-soluble vitamin K (menaquinone-7)2005

    • Author(s)
      Yasuhide Yanagisawa
    • Journal Title

      Journal of Food Biochemistry 29

      Pages: 267-277

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Additional effect of some nutrients and biological-active substances by Bacillus natto reatment of raw wheat2005

    • Author(s)
      Hiroyuki Sumi
    • Journal Title

      Brewing Society and Japan 100・6

      Pages: 449-453

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Anti-platelet aggregation and anti-blood coagulation activities of Bacillus subtilis natto component, dipicolinic acid2005

    • Author(s)
      Tadanori Ohsugi
    • Journal Title

      Food Science and Technology Research 11・3

      Pages: 308-310

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 血栓症予防に働く食品,及び機能成分2005

    • Author(s)
      須見洋行
    • Journal Title

      Priming Bio Medicine 1

      Pages: 16-24

    • NAID

      40015482260

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Natto bacillus contains a large amount of water-soluble vitamin K (menaquinone-7)2005

    • Author(s)
      Y.Yanagisawa, H.Sumi
    • Journal Title

      Journal of Food Biochemistry Vol.29, No.3

      Pages: 267-277

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 無蒸煮麦類の納豆菌処理による栄養価及び生理活性の付加2005

    • Author(s)
      須見洋行, 浅野倫和, 矢田貝智恵子
    • Journal Title

      日本醸造学会誌 100巻6号

      Pages: 449-453

    • NAID

      80017344526

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Anti-platelet aggregation and anti-blood coagulation activities of Bacillus subtilis natto component, dipicolinic acid2005

    • Author(s)
      T.Ohsugi, H.Sumi
    • Journal Title

      Food Science and Technology Research Vol.11, No.3

      Pages: 308-310

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 血栓症予防に働く食品,及び機能成分2005

    • Author(s)
      須見洋行
    • Journal Title

      倉敷芸術科学大学細胞病理学センター (印刷中)

    • NAID

      40015482260

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Natto Bacillus reacts as a vitamin K (menaquinone-7) supplement2004

    • Author(s)
      Sumi H., Yatagai C., Yanagisawa Y.
    • Journal Title

      J.Tempe Soc.Japan 10・1

      Pages: 17-20

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Natto Bacillus as an oral fibrinolytic agent : Natto activity and the ingestion of Bacillus subtilis natty2004

    • Author(s)
      Sumi H., Yanagisawa Y., Yatagai C., Saito J.
    • Journal Title

      Food Science and Technology Research

      Pages: 111-111

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Probiotics using natto bacillus for anti-thrombosis and anti-osteoporosis2004

    • Author(s)
      Sumi H., Yatagai C.
    • Journal Title

      XVIIth International Congress on Fibrinolysis and Proteolysis Melbourne, Australia March 21 99・12

      Pages: 867-872

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 骨粗鬆症予防剤としての黒豆納豆2004

    • Author(s)
      須見洋行, 矢田貝智恵子
    • Journal Title

      NEW FOOD INDUSTRY 46・9

      Pages: 1-5

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The functional food "natto" nattokinase, etc.2004

    • Author(s)
      Sumi U.
    • Journal Title

      International Symposium on Fermented Foods -Fermented Food and Health-

      Pages: 57-63

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Natto Bacillus as an oral fibrinolytic agent : Natto activity and the ingestion of Bacillus subtilis natto2004

    • Author(s)
      Hiroyuki Sumi
    • Journal Title

      Food Science and Technology Research 10・1

      Pages: 17-20

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Probiotics using natto bacillus for anti-thrombosis and anti-osteoporosis2004

    • Author(s)
      Hiroyuki Sumi
    • Journal Title

      XVIIth International Congress on Fibrinolysis and Proteolysis Melbourne, Australia March 21

      Pages: 111-111

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Production of nattokinase and vitamin K_2(menaquinone-7) using sake cake, in particular distilled cake, as the fermented material2004

    • Author(s)
      Hiroyuki Sumi
    • Journal Title

      Brewing Society and Japan 99・12

      Pages: 867-872

    • NAID

      10014244918

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Black soybean natto as osteoporosis prevention medicine2004

    • Author(s)
      Hiroyuki Sumi
    • Journal Title

      New Food Industry 46・9

      Pages: 1-5

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The functional food "natto" nattokinase, etc.2004

    • Author(s)
      Hiroyuki Sumi
    • Journal Title

      International Symposium on Fermented Foods -Fermented Food and Health-

      Pages: 57-63

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 酒粕類,特に焼酎蒸留粕を原料としたナットウキナーゼ及びビタミンK_2(メナキノン-7)の発酵生産2004

    • Author(s)
      須見洋行, 池田志織, 今井雅敏, 矢田貝智恵子
    • Journal Title

      日本醸造協会誌 99巻12号

      Pages: 867-872

    • NAID

      10014244918

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 骨粗鬆症予防剤としての黒豆納豆2004

    • Author(s)
      須見洋行, 矢田貝智恵子
    • Journal Title

      NEW FOOD INDUSTRY 46巻9号

      Pages: 1-5

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The functional food "natto" nattokinase, etc.2004

    • Author(s)
      H.Sumi
    • Journal Title

      International Symposium on Fermented Foods-Fermented Food and Health-

      Pages: 57-63

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 血栓溶解酵素ナットウキナーゼ生産に対するジピコリン酸の添加効果2003

    • Author(s)
      池田志織, 大杉忠則, 須見洋行
    • Journal Title

      日本食品科学工学会第50回大会講演要旨集

      Pages: 16-16

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 食品成分による血流改善と病気予防2003

    • Author(s)
      須見洋行
    • Journal Title

      Food Style 21 7・4

      Pages: 47-53

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] テンペ菌及び納豆菌が生産する血栓溶解酵素の酵素学的及び免疫学的性質2003

    • Author(s)
      須見洋行, 矢田貝智恵子, 岡本猛
    • Journal Title

      日本テンペ研究会誌 6

      Pages: 1-6

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Natto Bacillus as an oral fibrinolytic agent : Natto activity and the ingestion of Bacillus subtilis natty2003

    • Author(s)
      Sumi H., Yanagisawa Y., Yatagai C., Saito J.
    • Journal Title

      Food Science and Technology Research 6

      Pages: 7-19

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Additional effect of dipicolic acid on the productions of nattokinase, fibrinolytic enzyme2003

    • Author(s)
      Shiori Ikeda, Tadanori Ohsugi, Hiroyuki Sumi
    • Journal Title

      Abstracts of the 50^<th> congress on journal Japanese Society for Food Science and Technology

      Pages: 16-16

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Blood stream improvement and disease prevention by food factors2003

    • Author(s)
      Hiroyuki Sumi
    • Journal Title

      Food Style 21

      Pages: 47-53

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Zymology character and immunological character of fibrinolytic enzyme that Tempe bacterium and natto bacillus produce2003

    • Author(s)
      Hiroyuki Sumi
    • Journal Title

      Journal of Tempe Society Japan 6

      Pages: 1-6

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Natto Bacillus reacts as a vitamin K(menaquinone-7) supplement2003

    • Author(s)
      Hiroyuki Sumi
    • Journal Title

      Journal of Tempe Society Japan 6

      Pages: 7-19

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Activation of fibrinolysis(nattokinase) induced by dipicolinic acid and related compounds

    • Author(s)
      Shiori Ikeda
    • Journal Title

      Food Science and Technology Research (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] Soy in Health and Disease Prevention2005

    • Author(s)
      Hiroyuki Sumi, Chieko Yatagai
    • Total Pages
      313
    • Publisher
      Taylor & Francis
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] Soy in Health and Disease Prevention2005

    • Author(s)
      H.Sumi, C.Yatagai
    • Total Pages
      313
    • Publisher
      Taylor & Francis
    • Related Report
      2005 Annual Research Report
  • [Book] Soy in Health and Disease Prevention2005

    • Author(s)
      H.Sumi, C.Yatagai
    • Publisher
      Marcel Dekker, Inc. (USA)(In press)
    • Related Report
      2004 Annual Research Report
  • [Book] The Mediterranean League against Thromboembolic Diseases2004

    • Author(s)
      Yatagai C., Yanagisawa Y., Ohsugi T., Sumi H.
    • Total Pages
      122
    • Publisher
      Medimond (Italy)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] 機能性食品ガイド2004

    • Author(s)
      須見洋行
    • Total Pages
      511
    • Publisher
      講談社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Book] 健康食辞典2004

    • Author(s)
      須見洋行, 玉井嗣彦
    • Total Pages
      459
    • Publisher
      朝日新聞社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Book] Soy in Health and Disease Prevention2004

    • Author(s)
      Sumi H., Yatagai C.
    • Total Pages
      313
    • Publisher
      Taylor & Francis
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] The Mediterranean League against Thromboembolic Diseases2004

    • Author(s)
      Chieko Yatagai, Yasuhide Yanagisawa, Tadanori Ohsugi, Hiroyuki Sumi
    • Total Pages
      122
    • Publisher
      Medimond
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] Guide of functional food2004

    • Author(s)
      Hiroyuki Sumi
    • Total Pages
      511
    • Publisher
      Kodansha
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] Healthy food dictionary2004

    • Author(s)
      Hiroyuki Sumi, Tsuguhiko Tamai
    • Total Pages
      459
    • Publisher
      Asahi Shinbun Company
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] The Mediterranean League against Thromboembolic Diseases2004

    • Author(s)
      C.Yatagai, Y.Yanagisawa, T.Ohsugi, H.Sumi
    • Total Pages
      122
    • Publisher
      Medimond (Italy)
    • Related Report
      2004 Annual Research Report
  • [Publications] 池田志織, 大杉忠則, 須見洋行: "血栓溶解酵素ナットウキナーゼ生産に対するジピコリン酸の添加効果"日本食品科学工学会第50回大会講演要旨集. 16 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 須見洋行: "食品成分による血流改善と病気予防"Food Style 21. 7巻4号. 47-53 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 須見洋行, 矢田貝智恵子, 岡本猛: "テンペ菌及び納豆菌が生産する血栓溶解酵素の酵素学的及び免疫学的性質"日本テンペ研究会誌. 6巻. 1-6 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sumi H., Yatagai C., Yanagisawa Y.: "Natto Bacillus reacts as a vitamin K (menaquinone-7) supplement."J.Tempe Soc.Japan. 6巻. 7-19 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sumi H., Yanagisawa Y., Yatagai C., Saito J.: "Natto Bacillus as an oral fibrinolytic agent : Natto activity and the ingestion of Bacillus subtilis natto."Food Sci.Technol.Res.. 10巻1号(in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sumi H., Yatagai C.: "Probiotics using natto bacillus for anti-thrombosis and anti-osteoporosis"X VIIth International Congress on Fibrinolysis and Proteolysis Melbourne, Australia March 21. (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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