| Project/Area Number |
15500565
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Eating habits, studies on eating habits
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| Research Institution | DOSHISHA UNIVERSITY |
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Principal Investigator |
TSUDA Takanori Doshisha University, Office for Research Initiatives and Development, Associate Professor, 研究開発推進機構, 助教授 (90281568)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2003: ¥2,800,000 (Direct Cost: ¥2,800,000)
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| Keywords | anthocyanin / cyanidin 3-glucoside / cyanidin / adipocyte / obesity / diabetes / DNA microarray / シアニジン 3-グルコシド / 脂防細胞 / アディポネクチン / PPARγ / AMP-activated protein kinase |
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| Research Abstract |
In this study, we demonstrated that anthocyanins (cyanidin ; Cy or cyanidin 3-glucoside ; C3G) have the potency of a unique pharmacological function in isolated rat adipocytes. Treated adipocytes with anthocyanins enhanced adiponectin and leptin secretion and up-regulated the adipocyte specific gene expression without activation of PPARγ in isolated rat adipocytes. The gene expression of adiponectin was also up-regulated in white adipose tissue in mice fed an anthocyanin supplemented diet. As one of the possible mechanisms, AMP-activated protein kinase activation would be associated with these changes, nevertheless, the AMP: ATP ratio was significantly decreased by administration of the anthocyanins. To clarify the mechanism of the function, GeneChip microarray analysis was carried out. The up-regulated genes include lipid metabolism and signal transduction-related genes Based on the gene expression profile, we demonstrated the up-regulation of hormone sensitive lipase and enhancement of the lipolytic activity by the treatment of adipocytes with C3G or Cy. These data suggest that anthocyanins have a potency of unique therapeutic advantage, and also have important implications for preventing obesity and diabetes.
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